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The map label for this gene is fliM [H]

Identifier: 134300220

GI number: 134300220

Start: 2605175

End: 2606167

Strand: Reverse

Name: fliM [H]

Synonym: Dred_2381

Alternate gene names: 134300220

Gene position: 2606167-2605175 (Counterclockwise)

Preceding gene: 134300221

Following gene: 134300219

Centisome position: 72.23

GC content: 44.11

Gene sequence:

>993_bases
TTGTCCAAGGAAGTTCTGAGCCAAGGGGAAATAGACTCACTTTTGGCAGCATTGATGTCCGGGGATGTATCTCAGGAGAC
TGTTGAAACCAAGGAACAAAAGAAATACAAATCTTATGATTTCCGTAGGCCCAATAAATTCACCAAGGAACAGCTTCGCA
CCCTGCACGTATTACATGAGGGATATGCACGGCTACTTTCTAACTTTTTATCAGGCTATTTAAGGACACCCATCACCATT
GAAATTGCATCCATAGGTCAATTTACCTATGAAGAGTTTATCAATTCGGTTCCTAGCCCCACGGTTATGACGATTTTTAG
TTTGGCCCCACTGAAAGGGTCTGCACTAATGGAGACAAATTTGCAATTTTTGTTCCCCATCATTGATCTTCAATTTGGTG
GCCTAGGAGAAATGCCTCCTAAAACGAGGGAACTTACGGATATCGAATTATCGGTATCCAACCGGATTATCAAAAGACTG
TTAGATCACCTAACCATTAGTTGGAAAGATATTATGTCGGTATCTCCAAAAGTAGAATCCATTGATGCTAACCCCCATCT
TCACCAATTAATGTCCCCCAATGACATCGTTGTTGTGATCACTTTTTCCACCGGGGTGGCAGAGGAAGTCAGGGGTCTAA
TCAATCTTTGCTTGCCCTATAGTTTATTGGATCCGATCTTGTCGAAGTTCTCTATGGCTAACCAGTTTACCAAGACAACA
GAGCAGGATCAGAAGGAACTTATGGCGCTGGAGTACTGGCTGGGAGAGTCGGATGTGGCCATCAGTGTAGTCATGGGTGA
GGCTCAAATAACCGTTAAGGATTTCTTGCAGCTTCAAGTAGGAGATGTATTGCCTTTAGGCAGAGGTTTAACTCAGGATC
TTGATATGTATGTGGATGAAGAACTTAAGTACAAAGTGCAAGTGGGAACGGTCGGCCAGAGCTTGGCGGTTCAGGTGACC
TCACTGGCCGAGGAGGCGTGGGAAAATGTCTAA

Upstream 100 bases:

>100_bases
GCCCTTCCAGCAGGATCGTATTCTGCAAGCCATTGAACGTGTTCTTTCCCGTGCCTAGTCAAATATTTTTTTAGGATGGT
ATCGTAAAGGGGTGTAAAGG

Downstream 100 bases:

>100_bases
ACTATTAAGCCAGGAAGAAATTGACGCTTTAATGAAAGGGCAAGACCTGGGAAATGAACCGGATCCTCTGGGGAATCAGT
CAGAAATGTCTGATACAGTA

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSKEVLSQGEIDSLLAALMSGDVSQETVETKEQKKYKSYDFRRPNKFTKEQLRTLHVLHEGYARLLSNFLSGYLRTPITI
EIASIGQFTYEEFINSVPSPTVMTIFSLAPLKGSALMETNLQFLFPIIDLQFGGLGEMPPKTRELTDIELSVSNRIIKRL
LDHLTISWKDIMSVSPKVESIDANPHLHQLMSPNDIVVVITFSTGVAEEVRGLINLCLPYSLLDPILSKFSMANQFTKTT
EQDQKELMALEYWLGESDVAISVVMGEAQITVKDFLQLQVGDVLPLGRGLTQDLDMYVDEELKYKVQVGTVGQSLAVQVT
SLAEEAWENV

Sequences:

>Translated_330_residues
MSKEVLSQGEIDSLLAALMSGDVSQETVETKEQKKYKSYDFRRPNKFTKEQLRTLHVLHEGYARLLSNFLSGYLRTPITI
EIASIGQFTYEEFINSVPSPTVMTIFSLAPLKGSALMETNLQFLFPIIDLQFGGLGEMPPKTRELTDIELSVSNRIIKRL
LDHLTISWKDIMSVSPKVESIDANPHLHQLMSPNDIVVVITFSTGVAEEVRGLINLCLPYSLLDPILSKFSMANQFTKTT
EQDQKELMALEYWLGESDVAISVVMGEAQITVKDFLQLQVGDVLPLGRGLTQDLDMYVDEELKYKVQVGTVGQSLAVQVT
SLAEEAWENV
>Mature_329_residues
SKEVLSQGEIDSLLAALMSGDVSQETVETKEQKKYKSYDFRRPNKFTKEQLRTLHVLHEGYARLLSNFLSGYLRTPITIE
IASIGQFTYEEFINSVPSPTVMTIFSLAPLKGSALMETNLQFLFPIIDLQFGGLGEMPPKTRELTDIELSVSNRIIKRLL
DHLTISWKDIMSVSPKVESIDANPHLHQLMSPNDIVVVITFSTGVAEEVRGLINLCLPYSLLDPILSKFSMANQFTKTTE
QDQKELMALEYWLGESDVAISVVMGEAQITVKDFLQLQVGDVLPLGRGLTQDLDMYVDEELKYKVQVGTVGQSLAVQVTS
LAEEAWENV

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=330, Percent_Identity=27.8787878787879, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36984; Mature: 36853

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEVLSQGEIDSLLAALMSGDVSQETVETKEQKKYKSYDFRRPNKFTKEQLRTLHVLHE
CCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GYARLLSNFLSGYLRTPITIEIASIGQFTYEEFINSVPSPTVMTIFSLAPLKGSALMETN
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHCC
LQFLFPIIDLQFGGLGEMPPKTRELTDIELSVSNRIIKRLLDHLTISWKDIMSVSPKVES
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
IDANPHLHQLMSPNDIVVVITFSTGVAEEVRGLINLCLPYSLLDPILSKFSMANQFTKTT
CCCCCHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EQDQKELMALEYWLGESDVAISVVMGEAQITVKDFLQLQVGDVLPLGRGLTQDLDMYVDE
HHHHHHHHHHHHHCCCCCEEEEEEECCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
ELKYKVQVGTVGQSLAVQVTSLAEEAWENV
CCEEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKEVLSQGEIDSLLAALMSGDVSQETVETKEQKKYKSYDFRRPNKFTKEQLRTLHVLHE
CCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GYARLLSNFLSGYLRTPITIEIASIGQFTYEEFINSVPSPTVMTIFSLAPLKGSALMETN
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHCC
LQFLFPIIDLQFGGLGEMPPKTRELTDIELSVSNRIIKRLLDHLTISWKDIMSVSPKVES
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
IDANPHLHQLMSPNDIVVVITFSTGVAEEVRGLINLCLPYSLLDPILSKFSMANQFTKTT
CCCCCHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EQDQKELMALEYWLGESDVAISVVMGEAQITVKDFLQLQVGDVLPLGRGLTQDLDMYVDE
HHHHHHHHHHHHHCCCCCEEEEEEECCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
ELKYKVQVGTVGQSLAVQVTSLAEEAWENV
CCEEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]