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The map label for this gene is purB [H]

Identifier: 134300209

GI number: 134300209

Start: 2594530

End: 2595822

Strand: Reverse

Name: purB [H]

Synonym: Dred_2370

Alternate gene names: 134300209

Gene position: 2595822-2594530 (Counterclockwise)

Preceding gene: 134300210

Following gene: 134300208

Centisome position: 71.94

GC content: 46.64

Gene sequence:

>1293_bases
ATGATTGAACGTTATACCTTGCCCGAAATGAAGCACATCTGGTCTGACACAAACAAATTCCAAAAATGGCTGGACGTAGA
AATAGCTGCCTGCGAAGCTATGGTTGAATTGGGACAAATTCCTGCGGAGGCTCTGGAGAACATTAAGGCACGGGCTAGCT
TTTCTGTGGAACGTATTCTGGAAATTGAAGAAGTTACCAGACACGATGTGATTGCCTTCTTAACCTGCGTGGCTGAGAAC
GTAGGGGAAGATTCTAAATATATACACATGGGTCTTACATCCTCTGATGTAGTGGATACGGCCCAGTGTGTGCGCATGAA
AGAAGCCGGGGAACTAATCCTTAAAAAACTAGAACGCCTGCGCCAAGTACTGCTGGAAAAGGCCAAGGAACACAGACATA
CCATCATGATTGGCAGAACTCACGGTATTCATGCGGAGCCCATGACCTTTGGTCTGAAGATGCTACTTTGGGTAGCTGAA
ACCGAACGTAACATGGAGCGTCTACAGGACGCCATTAAGACAGTAAGTGTAGGAGCAATTTCCGGTGCAGTGGGAACCTA
TGCCAATATTGATCCCAAGATTGAGGCCCATGTTTGTAATAGGCTGGGGCTGAAGCCAGCTCGGGTTTCCACCCAAATTC
TACAACGGGACCGCCATGCAGATTATCTCTGCACAATGGCAGTCATCGCCGCCTCCCTAGACAAGTTTGCCACAGAGATT
CGCAATTTGCAGCGTACAGATATTTTAGAAGCGGAGGAGTACTTCAATAAAGGCCAAAAGGGTTCCTCAGCCATGCCCCA
CAAGCGGAATCCCATCACAGCAGAAAATGTATCTGGCCTGTCCCGGGTGGTGAAAGCCAATGCCATGGCAGCCTTGGATA
ATGTAACACTCTGGCACGAGAGAGACATTTCCCATTCTTCCGCTGAACGGGTCATTATCCCTGACAGTACCACTGCTTTA
GATTTTATGCTTTATCGTTTTACCGGTATTATGGAAAAACTGTTGGTGTACCCAGAAAATATGAAACGAAACCTGGAAAA
AACCCTGGGTTTGGTATTCTCCCAACGGGTGCTGTTGGCCTTGGTGGATAAAGGCTTAACCAGGGAACAGGCCTACGAAA
TGGTACAACGCAATGCTATGGAAGCCTGGCGTACTGGTACCAAGTTTATTGATTTATTGAAAGCGGACGCCGACATTATG
TCTCATATCACTTCAGAAGAGTTAACCGGACTCTTTGATTATAGCTATCACCTGTCTCATATTGACGATATTTATGCCCG
GTTTGGACTTTAA

Upstream 100 bases:

>100_bases
CAGGGAAGTGGAAGCCAAGGACAGCAAGCTAAAAGAGCTGGGTGTAGAGGGTTACCTGAATAACAAATAGGTTTATAGAC
GAAACGGAGGAACCTTATCG

Downstream 100 bases:

>100_bases
AAATTAATAACGTATTATCCCCGGCAACGGGGGTAATACGCTACCTTTTTAAAAAATAGATAAAGGGTTTGTTGAAATTA
CTGCAAGGAGCTGTCACAAG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MIERYTLPEMKHIWSDTNKFQKWLDVEIAACEAMVELGQIPAEALENIKARASFSVERILEIEEVTRHDVIAFLTCVAEN
VGEDSKYIHMGLTSSDVVDTAQCVRMKEAGELILKKLERLRQVLLEKAKEHRHTIMIGRTHGIHAEPMTFGLKMLLWVAE
TERNMERLQDAIKTVSVGAISGAVGTYANIDPKIEAHVCNRLGLKPARVSTQILQRDRHADYLCTMAVIAASLDKFATEI
RNLQRTDILEAEEYFNKGQKGSSAMPHKRNPITAENVSGLSRVVKANAMAALDNVTLWHERDISHSSAERVIIPDSTTAL
DFMLYRFTGIMEKLLVYPENMKRNLEKTLGLVFSQRVLLALVDKGLTREQAYEMVQRNAMEAWRTGTKFIDLLKADADIM
SHITSEELTGLFDYSYHLSHIDDIYARFGL

Sequences:

>Translated_430_residues
MIERYTLPEMKHIWSDTNKFQKWLDVEIAACEAMVELGQIPAEALENIKARASFSVERILEIEEVTRHDVIAFLTCVAEN
VGEDSKYIHMGLTSSDVVDTAQCVRMKEAGELILKKLERLRQVLLEKAKEHRHTIMIGRTHGIHAEPMTFGLKMLLWVAE
TERNMERLQDAIKTVSVGAISGAVGTYANIDPKIEAHVCNRLGLKPARVSTQILQRDRHADYLCTMAVIAASLDKFATEI
RNLQRTDILEAEEYFNKGQKGSSAMPHKRNPITAENVSGLSRVVKANAMAALDNVTLWHERDISHSSAERVIIPDSTTAL
DFMLYRFTGIMEKLLVYPENMKRNLEKTLGLVFSQRVLLALVDKGLTREQAYEMVQRNAMEAWRTGTKFIDLLKADADIM
SHITSEELTGLFDYSYHLSHIDDIYARFGL
>Mature_430_residues
MIERYTLPEMKHIWSDTNKFQKWLDVEIAACEAMVELGQIPAEALENIKARASFSVERILEIEEVTRHDVIAFLTCVAEN
VGEDSKYIHMGLTSSDVVDTAQCVRMKEAGELILKKLERLRQVLLEKAKEHRHTIMIGRTHGIHAEPMTFGLKMLLWVAE
TERNMERLQDAIKTVSVGAISGAVGTYANIDPKIEAHVCNRLGLKPARVSTQILQRDRHADYLCTMAVIAASLDKFATEI
RNLQRTDILEAEEYFNKGQKGSSAMPHKRNPITAENVSGLSRVVKANAMAALDNVTLWHERDISHSSAERVIIPDSTTAL
DFMLYRFTGIMEKLLVYPENMKRNLEKTLGLVFSQRVLLALVDKGLTREQAYEMVQRNAMEAWRTGTKFIDLLKADADIM
SHITSEELTGLFDYSYHLSHIDDIYARFGL

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=390, Percent_Identity=29.2307692307692, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=29.5039164490862, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1787376, Length=312, Percent_Identity=31.7307692307692, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790398, Length=410, Percent_Identity=24.8780487804878, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787896, Length=214, Percent_Identity=31.7757009345794, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=427, Percent_Identity=27.6346604215457, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564234, Length=355, Percent_Identity=29.2957746478873, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323391, Length=390, Percent_Identity=28.7179487179487, Blast_Score=155, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6324993, Length=187, Percent_Identity=32.0855614973262, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24647570, Length=399, Percent_Identity=27.3182957393484, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI221473854, Length=359, Percent_Identity=23.1197771587744, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI78706858, Length=359, Percent_Identity=23.1197771587744, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48750; Mature: 48750

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMKHIWSDTNKFQKWLDVEIAACEAMVELGQIPAEALENIKARASFSVERIL
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EIEEVTRHDVIAFLTCVAENVGEDSKYIHMGLTSSDVVDTAQCVRMKEAGELILKKLERL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RQVLLEKAKEHRHTIMIGRTHGIHAEPMTFGLKMLLWVAETERNMERLQDAIKTVSVGAI
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIDPKIEAHVCNRLGLKPARVSTQILQRDRHADYLCTMAVIAASLDKFATEI
HCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RNLQRTDILEAEEYFNKGQKGSSAMPHKRNPITAENVSGLSRVVKANAMAALDNVTLWHE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEC
RDISHSSAERVIIPDSTTALDFMLYRFTGIMEKLLVYPENMKRNLEKTLGLVFSQRVLLA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LVDKGLTREQAYEMVQRNAMEAWRTGTKFIDLLKADADIMSHITSEELTGLFDYSYHLSH
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDDIYARFGL
HHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMKHIWSDTNKFQKWLDVEIAACEAMVELGQIPAEALENIKARASFSVERIL
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EIEEVTRHDVIAFLTCVAENVGEDSKYIHMGLTSSDVVDTAQCVRMKEAGELILKKLERL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RQVLLEKAKEHRHTIMIGRTHGIHAEPMTFGLKMLLWVAETERNMERLQDAIKTVSVGAI
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIDPKIEAHVCNRLGLKPARVSTQILQRDRHADYLCTMAVIAASLDKFATEI
HCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RNLQRTDILEAEEYFNKGQKGSSAMPHKRNPITAENVSGLSRVVKANAMAALDNVTLWHE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEC
RDISHSSAERVIIPDSTTALDFMLYRFTGIMEKLLVYPENMKRNLEKTLGLVFSQRVLLA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LVDKGLTREQAYEMVQRNAMEAWRTGTKFIDLLKADADIMSHITSEELTGLFDYSYHLSH
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDDIYARFGL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]