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The map label for this gene is ligA

Identifier: 134300185

GI number: 134300185

Start: 2564983

End: 2566995

Strand: Reverse

Name: ligA

Synonym: Dred_2346

Alternate gene names: 134300185

Gene position: 2566995-2564983 (Counterclockwise)

Preceding gene: 134300186

Following gene: 134300184

Centisome position: 71.15

GC content: 50.12

Gene sequence:

>2013_bases
GTGGCAGATTTAAGCCAACAAAGCAAGGCCCGGGTGGAAGCACTGCGCCGGGAGATAAACCATCATAACTACCAGTACTA
TGTACTGGATCAACCTACTATTACTGACGCCCAGTACGATCAATTAATGCAGGAATTACTACGGCTGGAGGAGCGTCACC
CGGAGTTGGTAACCCCCGATTCCCCAACCCAGCGGGTTGGGGGGCAGGTGCAAAGGGGTTTTTCTTCTGTACCGCATAAA
ATACCGATGCTAAGTTTAGGCAATGCCTTTGGTGAAGGGGATTTGCGGGAATTTGATCGGCGTGTTCGTTCCTATCTGCC
CGGTGAAGAAGTAAAATATGTGGTGGAGCTTAAAATCGACGGCTTGGCCATTTCTCTCTGGTACGAGAAGGGCCTGCTGG
TCAGGGGTGCTACCCGGGGAGATGGCGAACTGGGAGAAGATATTACCATTAATCTAAAGACCATCAGGGCCATTCCTCTG
CGGCTAACGCAGGAAGTTCCCTTTCTAGAGGTCCGGGGCGAAGCCTATATGCCCAAGGATTCCTTTGTACGACTTAATGA
AGCCCGGGAGGAAGCCGGGGAACCGCTCTTTGCCAATCCACGCAATGCGGCGGCAGGTAGTCTACGCCAATTGGACCCTA
AAATTACGGCCGCCCGCAACCTGAGTGTGTTTATGTATGCCATCGGTTACATAGAAGGAGCTAAGCCCCCAAGCCATGCA
GAGAGCTTGGCCTGGTTAAAGGAACTGGGAATTCGCATTAACCCGGATCATCAAGTGTGCGGCAGCATCGACGAGGTTAT
TGACTTTATCAGCCAGTGGCAGGCTAAGCGTTACCAGTTGCCCTATGCCATTGATGGCATGGTAATCAAAGTGAACTCAT
TGGAGCAACAGCAGCGGCTGGGTACAACCATGAAAAGTCCCCGCTGGGCCATCGCCTATAAATTTCCCGCTGAGCAAGCC
GTCAGCACCATTAAGGATATCATCATACGGGTGGGACGTACCGGTGTATTAACCCCCACAGCCATCCTGGAGCCGGTTCA
ACTGGCGGGCACCACCGTTAGTAAAGCCACCTTGCATAATGAAGATATTATTCGCCAGAAGGATATCCGTATTGGAGATA
AAGCCCTGGTGCAGAAAGCAGGGGATATTATCCCGGAAATTGTTCAGGTATACCCTGAAAAACGAACTGGTAATGAAGTT
CCTTTTATCCTACCTGTTACCTGCCCGGAATGTGGTGCAGAGGTAGTTCGTGTGGCAGGAGAAGCGGCTCACCGCTGCAC
CAATGAAAACTGTTCAGCCAAATCCCGGGAGGGCATTATCCACTTTGTTTCCCGCAGTGCTATGGATATTATGGGGCTGG
GGGAAGGCATTGTGAACCAGCTTATTAAAGGCGGACTGGTGAAGGACCCTGCGGATCTCTATGATCTGAAATATGAGGAT
CTAATTCGGCAGGAACGAATGGGAGCCCGGTCTTCTCAAAAACTGCTGGCTGCCATTGAAGCCAGTAAAAATAATTCCCT
GGGTCAGCTTCTGTTTGGTCTGGGCATTCGCCATGTGGGTGAGCGAGCAGCTAAAATTCTGGCCCGCCAATTTGGTTCTA
TGCAAGCTCTGATGCAAGCAACGGCGGAAGATTTAACCGGCATTTCGGAAATTGGTCCTCGTATTGCCGAAAGTATAATG
GAATATTTTTCCAGGCAGGAGAACCAAGGTTTGATAGAGCGGTTGTCCAAGGCCGGTGTGAATATGTTGGAAGAGGTTGA
ACAGACAGAGTCCACTGATCAGACTTTGAGCGGTAAAACCTTTGTGGTAACTGGCACACTGGAAGGTTTTTCCCGGCAGG
AAGCTCAAAGGGCTATTGAGGAACGGGGCGGTAAAGTTTCCGGCAGTGTCAGTAAAAAAACCAACTATGTGGTGGTGGGA
GAAAACCCTGGTTCCAAGCATGATAAAGCACGGCAGTTGGGGATTACTATATTAACTGAGCAAGACTTTGTTAAACTATT
GCAGCAGCAATAA

Upstream 100 bases:

>100_bases
TTGGCACAATTGTTTTTTGCTCCCGGGGCTTACATTATCAAGTATGTAGCGGAAATTATTGCCCATGGAGGTTTTTAATT
GTTTTTCAGGGGGGAAGATT

Downstream 100 bases:

>100_bases
GTTCAAAGATAACTCCGGTCCTAGGTTAGGCCGGGGTTTTTTATTTAAGTACCGACAATTAAGGCCGAAAAGTAAGATAT
AGGAATAAAAAGAATCCTTT

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MADLSQQSKARVEALRREINHHNYQYYVLDQPTITDAQYDQLMQELLRLEERHPELVTPDSPTQRVGGQVQRGFSSVPHK
IPMLSLGNAFGEGDLREFDRRVRSYLPGEEVKYVVELKIDGLAISLWYEKGLLVRGATRGDGELGEDITINLKTIRAIPL
RLTQEVPFLEVRGEAYMPKDSFVRLNEAREEAGEPLFANPRNAAAGSLRQLDPKITAARNLSVFMYAIGYIEGAKPPSHA
ESLAWLKELGIRINPDHQVCGSIDEVIDFISQWQAKRYQLPYAIDGMVIKVNSLEQQQRLGTTMKSPRWAIAYKFPAEQA
VSTIKDIIIRVGRTGVLTPTAILEPVQLAGTTVSKATLHNEDIIRQKDIRIGDKALVQKAGDIIPEIVQVYPEKRTGNEV
PFILPVTCPECGAEVVRVAGEAAHRCTNENCSAKSREGIIHFVSRSAMDIMGLGEGIVNQLIKGGLVKDPADLYDLKYED
LIRQERMGARSSQKLLAAIEASKNNSLGQLLFGLGIRHVGERAAKILARQFGSMQALMQATAEDLTGISEIGPRIAESIM
EYFSRQENQGLIERLSKAGVNMLEEVEQTESTDQTLSGKTFVVTGTLEGFSRQEAQRAIEERGGKVSGSVSKKTNYVVVG
ENPGSKHDKARQLGITILTEQDFVKLLQQQ

Sequences:

>Translated_670_residues
MADLSQQSKARVEALRREINHHNYQYYVLDQPTITDAQYDQLMQELLRLEERHPELVTPDSPTQRVGGQVQRGFSSVPHK
IPMLSLGNAFGEGDLREFDRRVRSYLPGEEVKYVVELKIDGLAISLWYEKGLLVRGATRGDGELGEDITINLKTIRAIPL
RLTQEVPFLEVRGEAYMPKDSFVRLNEAREEAGEPLFANPRNAAAGSLRQLDPKITAARNLSVFMYAIGYIEGAKPPSHA
ESLAWLKELGIRINPDHQVCGSIDEVIDFISQWQAKRYQLPYAIDGMVIKVNSLEQQQRLGTTMKSPRWAIAYKFPAEQA
VSTIKDIIIRVGRTGVLTPTAILEPVQLAGTTVSKATLHNEDIIRQKDIRIGDKALVQKAGDIIPEIVQVYPEKRTGNEV
PFILPVTCPECGAEVVRVAGEAAHRCTNENCSAKSREGIIHFVSRSAMDIMGLGEGIVNQLIKGGLVKDPADLYDLKYED
LIRQERMGARSSQKLLAAIEASKNNSLGQLLFGLGIRHVGERAAKILARQFGSMQALMQATAEDLTGISEIGPRIAESIM
EYFSRQENQGLIERLSKAGVNMLEEVEQTESTDQTLSGKTFVVTGTLEGFSRQEAQRAIEERGGKVSGSVSKKTNYVVVG
ENPGSKHDKARQLGITILTEQDFVKLLQQQ
>Mature_669_residues
ADLSQQSKARVEALRREINHHNYQYYVLDQPTITDAQYDQLMQELLRLEERHPELVTPDSPTQRVGGQVQRGFSSVPHKI
PMLSLGNAFGEGDLREFDRRVRSYLPGEEVKYVVELKIDGLAISLWYEKGLLVRGATRGDGELGEDITINLKTIRAIPLR
LTQEVPFLEVRGEAYMPKDSFVRLNEAREEAGEPLFANPRNAAAGSLRQLDPKITAARNLSVFMYAIGYIEGAKPPSHAE
SLAWLKELGIRINPDHQVCGSIDEVIDFISQWQAKRYQLPYAIDGMVIKVNSLEQQQRLGTTMKSPRWAIAYKFPAEQAV
STIKDIIIRVGRTGVLTPTAILEPVQLAGTTVSKATLHNEDIIRQKDIRIGDKALVQKAGDIIPEIVQVYPEKRTGNEVP
FILPVTCPECGAEVVRVAGEAAHRCTNENCSAKSREGIIHFVSRSAMDIMGLGEGIVNQLIKGGLVKDPADLYDLKYEDL
IRQERMGARSSQKLLAAIEASKNNSLGQLLFGLGIRHVGERAAKILARQFGSMQALMQATAEDLTGISEIGPRIAESIME
YFSRQENQGLIERLSKAGVNMLEEVEQTESTDQTLSGKTFVVTGTLEGFSRQEAQRAIEERGGKVSGSVSKKTNYVVVGE
NPGSKHDKARQLGITILTEQDFVKLLQQQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=105, Percent_Identity=41.9047619047619, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788750, Length=659, Percent_Identity=46.4339908952959, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI87082305, Length=416, Percent_Identity=24.5192307692308, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_DESRM (A4J703)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113681.1
- ProteinModelPortal:   A4J703
- STRING:   A4J703
- GeneID:   4956696
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2346
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   A4J703
- BioCyc:   DRED349161:DRED_2346-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74381; Mature: 74250

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 118-118 BINDING 116-116 BINDING 139-139 BINDING 174-174 BINDING 290-290 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLSQQSKARVEALRREINHHNYQYYVLDQPTITDAQYDQLMQELLRLEERHPELVTPD
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SPTQRVGGQVQRGFSSVPHKIPMLSLGNAFGEGDLREFDRRVRSYLPGEEVKYVVELKID
CCHHHHCHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEC
GLAISLWYEKGLLVRGATRGDGELGEDITINLKTIRAIPLRLTQEVPFLEVRGEAYMPKD
CEEEEEEECCCEEEECCCCCCCCCCCCEEEEEEEEEEECHHHHCCCCEEEECCCEECCHH
SFVRLNEAREEAGEPLFANPRNAAAGSLRQLDPKITAARNLSVFMYAIGYIEGAKPPSHA
HHEEHHHHHHHHCCCCCCCCCCCHHCCHHHCCCCHHHHCCHHHHHHHHHHHCCCCCCHHH
ESLAWLKELGIRINPDHQVCGSIDEVIDFISQWQAKRYQLPYAIDGMVIKVNSLEQQQRL
HHHHHHHHCCCEECCCHHHHCCHHHHHHHHHHHHHHHCCCCEEECCEEEEECCHHHHHHH
GTTMKSPRWAIAYKFPAEQAVSTIKDIIIRVGRTGVLTPTAILEPVQLAGTTVSKATLHN
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHCCCCHHHHHHCC
EDIIRQKDIRIGDKALVQKAGDIIPEIVQVYPEKRTGNEVPFILPVTCPECGAEVVRVAG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
EAAHRCTNENCSAKSREGIIHFVSRSAMDIMGLGEGIVNQLIKGGLVKDPADLYDLKYED
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHCCCHHH
LIRQERMGARSSQKLLAAIEASKNNSLGQLLFGLGIRHVGERAAKILARQFGSMQALMQA
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAEDLTGISEIGPRIAESIMEYFSRQENQGLIERLSKAGVNMLEEVEQTESTDQTLSGKT
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FVVTGTLEGFSRQEAQRAIEERGGKVSGSVSKKTNYVVVGENPGSKHDKARQLGITILTE
EEEEECCCCCHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCCCCHHHHHHCEEEECH
QDFVKLLQQQ
HHHHHHHHCC
>Mature Secondary Structure 
ADLSQQSKARVEALRREINHHNYQYYVLDQPTITDAQYDQLMQELLRLEERHPELVTPD
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SPTQRVGGQVQRGFSSVPHKIPMLSLGNAFGEGDLREFDRRVRSYLPGEEVKYVVELKID
CCHHHHCHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEC
GLAISLWYEKGLLVRGATRGDGELGEDITINLKTIRAIPLRLTQEVPFLEVRGEAYMPKD
CEEEEEEECCCEEEECCCCCCCCCCCCEEEEEEEEEEECHHHHCCCCEEEECCCEECCHH
SFVRLNEAREEAGEPLFANPRNAAAGSLRQLDPKITAARNLSVFMYAIGYIEGAKPPSHA
HHEEHHHHHHHHCCCCCCCCCCCHHCCHHHCCCCHHHHCCHHHHHHHHHHHCCCCCCHHH
ESLAWLKELGIRINPDHQVCGSIDEVIDFISQWQAKRYQLPYAIDGMVIKVNSLEQQQRL
HHHHHHHHCCCEECCCHHHHCCHHHHHHHHHHHHHHHCCCCEEECCEEEEECCHHHHHHH
GTTMKSPRWAIAYKFPAEQAVSTIKDIIIRVGRTGVLTPTAILEPVQLAGTTVSKATLHN
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHCCCCHHHHHHCC
EDIIRQKDIRIGDKALVQKAGDIIPEIVQVYPEKRTGNEVPFILPVTCPECGAEVVRVAG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
EAAHRCTNENCSAKSREGIIHFVSRSAMDIMGLGEGIVNQLIKGGLVKDPADLYDLKYED
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHCCCHHH
LIRQERMGARSSQKLLAAIEASKNNSLGQLLFGLGIRHVGERAAKILARQFGSMQALMQA
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAEDLTGISEIGPRIAESIMEYFSRQENQGLIERLSKAGVNMLEEVEQTESTDQTLSGKT
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FVVTGTLEGFSRQEAQRAIEERGGKVSGSVSKKTNYVVVGENPGSKHDKARQLGITILTE
EEEEECCCCCHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCCCCHHHHHHCEEEECH
QDFVKLLQQQ
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA