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The map label for this gene is braC [H]

Identifier: 134300082

GI number: 134300082

Start: 2448829

End: 2450013

Strand: Reverse

Name: braC [H]

Synonym: Dred_2240

Alternate gene names: 134300082

Gene position: 2450013-2448829 (Counterclockwise)

Preceding gene: 134300083

Following gene: 134300081

Centisome position: 67.9

GC content: 41.27

Gene sequence:

>1185_bases
ATGAAAAAACATTTTAGATTAGCCGCCTTGCTAGTAGCCCTAGTCATGCTAGCCGGATTGGTTAGTGGATGTGGCGGCGG
CGGAGAAGAAAAGAAAGCAGCCGATGCCAATGAAATCGTCATTGGTGGTAACCTTGAATTGAGCGGTCCTGTTGCTACCT
TCGGTACAGCCATGAAAAATGGCGCCGAGATGTATTTTGAAGAAGTAAATGCTGCTGGTGGAGTTCTTGGAAAGAAAATT
AAGTTTAATGTTATGGATAATAAATCAGATGCAACAGAAGCTGCGAACGTAGCAACTCGCTTAATTACCCAAGACAAAGT
AGTAGCAGTAATGGGTGCTGCAACTTCCGGCAATACCATGGGTTTTATGCAAGTGGCTACAGATAACAAAGTTCCCATTA
TCACTCCCTCTGGTACTGCCTTGGAAGTAACCGTTGATCCTAACACTAAAAAGGTTCGCGACTATGTTTTTCGTACCTGC
TTTATCGATCCTTTCCAAGGCATTGTAATGGCTAACTTTGCCACTAACGATTTGAAAGCTAAGACAGCTGTTCTTTATGT
TGATAACCAGTCTCCCTATGCCAAAGGTCTGGCCCAGTTCTTTAAAGATTCCTTCATTAAACAAGGTGGCAAAATTGTTG
GTGAGGAAGCCTTTATGCCAGAAGACCAAGATTTTAAAGCAACTTTAACAAAAATAAAAGGTTTAAATCCAGATGTAATC
TATGTTCCTGCTTATTATGAGCCAGTAGGTAAGATCGTTAAGCAAGGTAGAGAAATTGGTATTACAGTTCCCTTCCTGGG
TGGCGACGGTTGGGATTCTCCTAAATTACCGGAAATTGCTGGTGGTGCAGCCCTAAACAATACCTATTTTAGCAATCACT
ATTCATCTCAGAAAGATGACCCTGAAATTAAAGGTTTTGTCGAAAAATATCAAGCAAAATTTGGCCAAGTTCCCGATACT
TTTGCAGCCCTTGGTTATGATACTGCCGTATTATTGGTAGATGCCATCAAGCGTTCCGAAGACGCATCTCCTGAGAAAAT
AAAAGAAGCACTGGCCAATGCCAAGGATGTGCAAGCTATAACTGGAAAACTGTCATTTGATGAGAACCATAACCCCATAA
AAGGTGCTGTTATCCTAGAAATGAAAGATGGCAAACAAGTCTATAAAACCTACGTACAACCTTAA

Upstream 100 bases:

>100_bases
TTTTATCATTTTTATTTATAAATTATGGCGGGAGGTGTTTAAAAGACTGACAGGGGATTGGTTTTGTTTAATGTTTAGTT
AACGAATGAGGAGGGGTAAA

Downstream 100 bases:

>100_bases
GGAAACCAAGCCTAAATAAAAGATAGTGAGGGAGTCTCACTCCCTCACTATCTTTTTAAGCAACTCTAAAACAACTGATA
GAGGTGGCCGCTTTGGAAGT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKKHFRLAALLVALVMLAGLVSGCGGGGEEKKAADANEIVIGGNLELSGPVATFGTAMKNGAEMYFEEVNAAGGVLGKKI
KFNVMDNKSDATEAANVATRLITQDKVVAVMGAATSGNTMGFMQVATDNKVPIITPSGTALEVTVDPNTKKVRDYVFRTC
FIDPFQGIVMANFATNDLKAKTAVLYVDNQSPYAKGLAQFFKDSFIKQGGKIVGEEAFMPEDQDFKATLTKIKGLNPDVI
YVPAYYEPVGKIVKQGREIGITVPFLGGDGWDSPKLPEIAGGAALNNTYFSNHYSSQKDDPEIKGFVEKYQAKFGQVPDT
FAALGYDTAVLLVDAIKRSEDASPEKIKEALANAKDVQAITGKLSFDENHNPIKGAVILEMKDGKQVYKTYVQP

Sequences:

>Translated_394_residues
MKKHFRLAALLVALVMLAGLVSGCGGGGEEKKAADANEIVIGGNLELSGPVATFGTAMKNGAEMYFEEVNAAGGVLGKKI
KFNVMDNKSDATEAANVATRLITQDKVVAVMGAATSGNTMGFMQVATDNKVPIITPSGTALEVTVDPNTKKVRDYVFRTC
FIDPFQGIVMANFATNDLKAKTAVLYVDNQSPYAKGLAQFFKDSFIKQGGKIVGEEAFMPEDQDFKATLTKIKGLNPDVI
YVPAYYEPVGKIVKQGREIGITVPFLGGDGWDSPKLPEIAGGAALNNTYFSNHYSSQKDDPEIKGFVEKYQAKFGQVPDT
FAALGYDTAVLLVDAIKRSEDASPEKIKEALANAKDVQAITGKLSFDENHNPIKGAVILEMKDGKQVYKTYVQP
>Mature_394_residues
MKKHFRLAALLVALVMLAGLVSGCGGGGEEKKAADANEIVIGGNLELSGPVATFGTAMKNGAEMYFEEVNAAGGVLGKKI
KFNVMDNKSDATEAANVATRLITQDKVVAVMGAATSGNTMGFMQVATDNKVPIITPSGTALEVTVDPNTKKVRDYVFRTC
FIDPFQGIVMANFATNDLKAKTAVLYVDNQSPYAKGLAQFFKDSFIKQGGKIVGEEAFMPEDQDFKATLTKIKGLNPDVI
YVPAYYEPVGKIVKQGREIGITVPFLGGDGWDSPKLPEIAGGAALNNTYFSNHYSSQKDDPEIKGFVEKYQAKFGQVPDT
FAALGYDTAVLLVDAIKRSEDASPEKIKEALANAKDVQAITGKLSFDENHNPIKGAVILEMKDGKQVYKTYVQP

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=327, Percent_Identity=26.605504587156, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI48994941, Length=327, Percent_Identity=29.0519877675841, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42308; Mature: 42308

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHFRLAALLVALVMLAGLVSGCGGGGEEKKAADANEIVIGGNLELSGPVATFGTAMKN
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEECCCHHHHHHHHHC
GAEMYFEEVNAAGGVLGKKIKFNVMDNKSDATEAANVATRLITQDKVVAVMGAATSGNTM
CHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCE
GFMQVATDNKVPIITPSGTALEVTVDPNTKKVRDYVFRTCFIDPFQGIVMANFATNDLKA
EEEEEECCCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KTAVLYVDNQSPYAKGLAQFFKDSFIKQGGKIVGEEAFMPEDQDFKATLTKIKGLNPDVI
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCEE
YVPAYYEPVGKIVKQGREIGITVPFLGGDGWDSPKLPEIAGGAALNNTYFSNHYSSQKDD
EECCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCEECCCEECCCCCCCCCC
PEIKGFVEKYQAKFGQVPDTFAALGYDTAVLLVDAIKRSEDASPEKIKEALANAKDVQAI
HHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHH
TGKLSFDENHNPIKGAVILEMKDGKQVYKTYVQP
HEEEECCCCCCCCCEEEEEEECCCHHHHHHHCCC
>Mature Secondary Structure
MKKHFRLAALLVALVMLAGLVSGCGGGGEEKKAADANEIVIGGNLELSGPVATFGTAMKN
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEECCCHHHHHHHHHC
GAEMYFEEVNAAGGVLGKKIKFNVMDNKSDATEAANVATRLITQDKVVAVMGAATSGNTM
CHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCE
GFMQVATDNKVPIITPSGTALEVTVDPNTKKVRDYVFRTCFIDPFQGIVMANFATNDLKA
EEEEEECCCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KTAVLYVDNQSPYAKGLAQFFKDSFIKQGGKIVGEEAFMPEDQDFKATLTKIKGLNPDVI
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCEE
YVPAYYEPVGKIVKQGREIGITVPFLGGDGWDSPKLPEIAGGAALNNTYFSNHYSSQKDD
EECCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCEECCCEECCCCCCCCCC
PEIKGFVEKYQAKFGQVPDTFAALGYDTAVLLVDAIKRSEDASPEKIKEALANAKDVQAI
HHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHH
TGKLSFDENHNPIKGAVILEMKDGKQVYKTYVQP
HEEEECCCCCCCCCEEEEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]