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The map label for this gene is cofH [H]

Identifier: 134300039

GI number: 134300039

Start: 2400213

End: 2401316

Strand: Reverse

Name: cofH [H]

Synonym: Dred_2195

Alternate gene names: 134300039

Gene position: 2401316-2400213 (Counterclockwise)

Preceding gene: 134300040

Following gene: 134300038

Centisome position: 66.55

GC content: 41.58

Gene sequence:

>1104_bases
ATGGACTATCTTTTTGCTAACAGTGAGCTTATTGATATTAAGGAAAAGGTAGAAAAAGGGGAGAGGCTTAGTAAGGAAGA
TGGCCTTCGATTATATCAGTCCAACGAATTGCTGGCCATTGGTTATCTGGCTGACATGGTTAATAAACGGAAAAATGGAG
ATAGGATATACTTTATTGTAAATCGTCATATTAATCACACCAATATCTGTGAAAACTTGTGTAAGTTCTGTGCCTTTGGC
CGAAAAAAAGATGCTCCTGGGGCCTATGCATTATCCCTAGACGAAATAGAGACCAAGGCAAAAGAGTGTAATGGGCTGAA
AGTTTCCGAAATACACATAGTAGGAGGCTTAAATCCTGATTTAAAACTAGATTATTACGTGAAAATGTTACAGCGTGTTC
AAGCTGCTGCACCAGGGACCATTATCCAATCCTTAACCGCTGTAGAAGTGGATTATTTAGCGAAAATCCATAACATGTCC
CTAGGAGAAGTATTGCAAACATTAAAGGGCGCAGGCCTTGCATCTTTACCCGGCGGTGGAGCAGAGGTCTTTTCTCAGCG
GGTGAGGGACTTGGTTTGTGAGAAAAAAATCAGTGGCGACCGATGGTTAGAGGTTCATGAAGCAGCCCATCGAATTGGTA
TGAAAACCAATGCCACCATGCTATATGGACATGTTGAGACACTGGAAGAAAGAATCGATCATCTGATTCGTCTAAGGCAG
TTGCAGGATAAGACCGGAGGTTTCTTAGCCTTTATTCCGCTGGCCTTCCATCCGAAGAATACAGCTTTAGATTGCCAAAA
TATATCAGGAACTACGGGCTATGATGACCTTAAAACCTTGGCCCTGGCAAGGCTAATGTTAGATAACTTTGATCATATAA
AAGCCTACTGGATAATGATCGGCCTTAAAATTGCCCAGGTGTCTTTATCCTTTGGTGCTAACGACCTAGATGGAACGGTA
ATAGAAGAGCAAATTGTTCATGATGCCGGAGCAGAAACTGCACAGTTTATGGCAAAGGCAAAATTAATAAATATAATAAA
GGCTGCAGGTAAGCAACCAGTTGAACGAGATACAATGTACAATGTTCTTGGGGAGGGTTTTTAG

Upstream 100 bases:

>100_bases
TTTGCCTGTACCTTTGTTGATATTCTGTTGCATTAAAGATAACACCCACGGCTTAAAGGAGCCGGGGATATTTTTTAATG
ACTGTAGAAAGGAGAACAAC

Downstream 100 bases:

>100_bases
TGGGCAAAATACGGTTTGGGCAGGTAGATTACCTAAATACGTTGCCAGTCTTTCATGCTTTAGAAGATGGCCTCCTGCCC
TTTGATGGAGAATTAATCAA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MDYLFANSELIDIKEKVEKGERLSKEDGLRLYQSNELLAIGYLADMVNKRKNGDRIYFIVNRHINHTNICENLCKFCAFG
RKKDAPGAYALSLDEIETKAKECNGLKVSEIHIVGGLNPDLKLDYYVKMLQRVQAAAPGTIIQSLTAVEVDYLAKIHNMS
LGEVLQTLKGAGLASLPGGGAEVFSQRVRDLVCEKKISGDRWLEVHEAAHRIGMKTNATMLYGHVETLEERIDHLIRLRQ
LQDKTGGFLAFIPLAFHPKNTALDCQNISGTTGYDDLKTLALARLMLDNFDHIKAYWIMIGLKIAQVSLSFGANDLDGTV
IEEQIVHDAGAETAQFMAKAKLINIIKAAGKQPVERDTMYNVLGEGF

Sequences:

>Translated_367_residues
MDYLFANSELIDIKEKVEKGERLSKEDGLRLYQSNELLAIGYLADMVNKRKNGDRIYFIVNRHINHTNICENLCKFCAFG
RKKDAPGAYALSLDEIETKAKECNGLKVSEIHIVGGLNPDLKLDYYVKMLQRVQAAAPGTIIQSLTAVEVDYLAKIHNMS
LGEVLQTLKGAGLASLPGGGAEVFSQRVRDLVCEKKISGDRWLEVHEAAHRIGMKTNATMLYGHVETLEERIDHLIRLRQ
LQDKTGGFLAFIPLAFHPKNTALDCQNISGTTGYDDLKTLALARLMLDNFDHIKAYWIMIGLKIAQVSLSFGANDLDGTV
IEEQIVHDAGAETAQFMAKAKLINIIKAAGKQPVERDTMYNVLGEGF
>Mature_367_residues
MDYLFANSELIDIKEKVEKGERLSKEDGLRLYQSNELLAIGYLADMVNKRKNGDRIYFIVNRHINHTNICENLCKFCAFG
RKKDAPGAYALSLDEIETKAKECNGLKVSEIHIVGGLNPDLKLDYYVKMLQRVQAAAPGTIIQSLTAVEVDYLAKIHNMS
LGEVLQTLKGAGLASLPGGGAEVFSQRVRDLVCEKKISGDRWLEVHEAAHRIGMKTNATMLYGHVETLEERIDHLIRLRQ
LQDKTGGFLAFIPLAFHPKNTALDCQNISGTTGYDDLKTLALARLMLDNFDHIKAYWIMIGLKIAQVSLSFGANDLDGTV
IEEQIVHDAGAETAQFMAKAKLINIIKAAGKQPVERDTMYNVLGEGF

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 40812; Mature: 40812

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLFANSELIDIKEKVEKGERLSKEDGLRLYQSNELLAIGYLADMVNKRKNGDRIYFIV
CCCEECCCCEEHHHHHHHHHHCCCHHCCCEEEECCCEEEHHHHHHHHHCCCCCCEEEEEE
NRHINHTNICENLCKFCAFGRKKDAPGAYALSLDEIETKAKECNGLKVSEIHIVGGLNPD
ECCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEEEEECCCCC
LKLDYYVKMLQRVQAAAPGTIIQSLTAVEVDYLAKIHNMSLGEVLQTLKGAGLASLPGGG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCH
AEVFSQRVRDLVCEKKISGDRWLEVHEAAHRIGMKTNATMLYGHVETLEERIDHLIRLRQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHH
LQDKTGGFLAFIPLAFHPKNTALDCQNISGTTGYDDLKTLALARLMLDNFDHIKAYWIMI
HHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLKIAQVSLSFGANDLDGTVIEEQIVHDAGAETAQFMAKAKLINIIKAAGKQPVERDTMY
HHHHEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
NVLGEGF
HHHCCCC
>Mature Secondary Structure
MDYLFANSELIDIKEKVEKGERLSKEDGLRLYQSNELLAIGYLADMVNKRKNGDRIYFIV
CCCEECCCCEEHHHHHHHHHHCCCHHCCCEEEECCCEEEHHHHHHHHHCCCCCCEEEEEE
NRHINHTNICENLCKFCAFGRKKDAPGAYALSLDEIETKAKECNGLKVSEIHIVGGLNPD
ECCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEEEEECCCCC
LKLDYYVKMLQRVQAAAPGTIIQSLTAVEVDYLAKIHNMSLGEVLQTLKGAGLASLPGGG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCH
AEVFSQRVRDLVCEKKISGDRWLEVHEAAHRIGMKTNATMLYGHVETLEERIDHLIRLRQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHH
LQDKTGGFLAFIPLAFHPKNTALDCQNISGTTGYDDLKTLALARLMLDNFDHIKAYWIMI
HHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLKIAQVSLSFGANDLDGTVIEEQIVHDAGAETAQFMAKAKLINIIKAAGKQPVERDTMY
HHHHEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
NVLGEGF
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]