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The map label for this gene is ykoU [H]

Identifier: 134299833

GI number: 134299833

Start: 2181598

End: 2182509

Strand: Reverse

Name: ykoU [H]

Synonym: Dred_1986

Alternate gene names: 134299833

Gene position: 2182509-2181598 (Counterclockwise)

Preceding gene: 134299834

Following gene: 134299832

Centisome position: 60.49

GC content: 38.49

Gene sequence:

>912_bases
GTGAGCACTATATCAACAACAGAGACAAGAATAGAGGGGAAAAATATAAAACTTACTAATTTAAATAAGTTAATGTGGCC
CGAGGGCTTGACCAAAGCACACCTAGTAAAGTATTATACGGACGTTGCGCCTTATTTACTTCCTTATATAAAAGAGCGCC
CCTTGGTAATGAAACGTTATCCAGATGGTATTGAAGGGGGATTTTTCTATCAGAAAGAATGTCCCAATTATGCTCCGGAA
TGGATAAAGACAGAGTCCATACGACATTCAGATAAGACAGTGAATTATATAATTTGTGAAGATGCTGCTACCTTGGTGTG
GTTAGCCAATCAGGGATGTATAGAAATACATGCATGGTTATGCAAACAAAGTCATATAGAATATCCAGATATTGCTATTA
TTGATTTAGACCCGGGAGAAGAGGCAAAGTTTCGAGATGTATTAGAAGTGGCATTGGTGATCAAAAATGCGTTAGACCAC
TTAAAGCTGGTTGGTTACCCGAAAACCTCTGGGGCGACTGGTATTCATATTTTTATTCCTCTTAGGCCGGTATATACCTT
TCAACAGGTAACCAAGGCAATGGGTTTAATTGCACAAACAGTAGCAGATGCTTTCTCTGAGAAAGCAACCATTGAGAGGT
CCTTAGCTAAAAGGGATAAAAGAAAAATCTACGTTGATTACCTACAGAATACCAGAGGTAAAAGCATGGCATGGACCTAT
AGTTTGCGACCTATTGCTGGTGCACCCATATCAACACCAGTGATGTGGGAGGAAATTGAAAAACTGCATTTAAAGCCAAG
TGAATTTAATATTTATACAATGGGAAATAGGCTTAAATTTTTTGGGGACTTACACCAAGATCTTTTGCGAAAGCATCAGG
ACATAGGCCATATTTTAAGGTTGATCTCATAA

Upstream 100 bases:

>100_bases
CTACTTAACATAAAATTCTTTTTATAGCCCGGCATCTTTGCCGGGCTATAATTTTTTTAAAGATGGGGACAAAATATAAA
AAGTGAAGGGAGGCGTAAAG

Downstream 100 bases:

>100_bases
ATTTTAGAAAAAAAAGATTGTATGGAAGGAAAATCCAATTACAATCCCGAAAGTGGTAGAAATCCAGTAACCGGGGTGGG
TAATTTGGATAATCGTTTAG

Product: DNA primase, small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSTISTTETRIEGKNIKLTNLNKLMWPEGLTKAHLVKYYTDVAPYLLPYIKERPLVMKRYPDGIEGGFFYQKECPNYAPE
WIKTESIRHSDKTVNYIICEDAATLVWLANQGCIEIHAWLCKQSHIEYPDIAIIDLDPGEEAKFRDVLEVALVIKNALDH
LKLVGYPKTSGATGIHIFIPLRPVYTFQQVTKAMGLIAQTVADAFSEKATIERSLAKRDKRKIYVDYLQNTRGKSMAWTY
SLRPIAGAPISTPVMWEEIEKLHLKPSEFNIYTMGNRLKFFGDLHQDLLRKHQDIGHILRLIS

Sequences:

>Translated_303_residues
MSTISTTETRIEGKNIKLTNLNKLMWPEGLTKAHLVKYYTDVAPYLLPYIKERPLVMKRYPDGIEGGFFYQKECPNYAPE
WIKTESIRHSDKTVNYIICEDAATLVWLANQGCIEIHAWLCKQSHIEYPDIAIIDLDPGEEAKFRDVLEVALVIKNALDH
LKLVGYPKTSGATGIHIFIPLRPVYTFQQVTKAMGLIAQTVADAFSEKATIERSLAKRDKRKIYVDYLQNTRGKSMAWTY
SLRPIAGAPISTPVMWEEIEKLHLKPSEFNIYTMGNRLKFFGDLHQDLLRKHQDIGHILRLIS
>Mature_302_residues
STISTTETRIEGKNIKLTNLNKLMWPEGLTKAHLVKYYTDVAPYLLPYIKERPLVMKRYPDGIEGGFFYQKECPNYAPEW
IKTESIRHSDKTVNYIICEDAATLVWLANQGCIEIHAWLCKQSHIEYPDIAIIDLDPGEEAKFRDVLEVALVIKNALDHL
KLVGYPKTSGATGIHIFIPLRPVYTFQQVTKAMGLIAQTVADAFSEKATIERSLAKRDKRKIYVDYLQNTRGKSMAWTYS
LRPIAGAPISTPVMWEEIEKLHLKPSEFNIYTMGNRLKFFGDLHQDLLRKHQDIGHILRLIS

Specific function: Probably involved in the repair of DNA double-strand breaks by non-homologous-end joining (NHEJ) during spore germination [H]

COG id: COG3285

COG function: function code L; Predicted eukaryotic-type DNA primase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR014146
- InterPro:   IPR014145
- InterPro:   IPR014143 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 34782; Mature: 34651

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTISTTETRIEGKNIKLTNLNKLMWPEGLTKAHLVKYYTDVAPYLLPYIKERPLVMKRY
CCCCCCCHHEECCCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECC
PDGIEGGFFYQKECPNYAPEWIKTESIRHSDKTVNYIICEDAATLVWLANQGCIEIHAWL
CCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCEEHHHHH
CKQSHIEYPDIAIIDLDPGEEAKFRDVLEVALVIKNALDHLKLVGYPKTSGATGIHIFIP
HHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEE
LRPVYTFQQVTKAMGLIAQTVADAFSEKATIERSLAKRDKRKIYVDYLQNTRGKSMAWTY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCEEEEE
SLRPIAGAPISTPVMWEEIEKLHLKPSEFNIYTMGNRLKFFGDLHQDLLRKHQDIGHILR
ECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LIS
HCC
>Mature Secondary Structure 
STISTTETRIEGKNIKLTNLNKLMWPEGLTKAHLVKYYTDVAPYLLPYIKERPLVMKRY
CCCCCCHHEECCCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECC
PDGIEGGFFYQKECPNYAPEWIKTESIRHSDKTVNYIICEDAATLVWLANQGCIEIHAWL
CCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCEEHHHHH
CKQSHIEYPDIAIIDLDPGEEAKFRDVLEVALVIKNALDHLKLVGYPKTSGATGIHIFIP
HHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEE
LRPVYTFQQVTKAMGLIAQTVADAFSEKATIERSLAKRDKRKIYVDYLQNTRGKSMAWTY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCEEEEE
SLRPIAGAPISTPVMWEEIEKLHLKPSEFNIYTMGNRLKFFGDLHQDLLRKHQDIGHILR
ECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LIS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]