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The map label for this gene is hslU

Identifier: 134299827

GI number: 134299827

Start: 2173752

End: 2175152

Strand: Reverse

Name: hslU

Synonym: Dred_1980

Alternate gene names: 134299827

Gene position: 2175152-2173752 (Counterclockwise)

Preceding gene: 134299828

Following gene: 134299826

Centisome position: 60.29

GC content: 41.54

Gene sequence:

>1401_bases
ATGGAAGCCCTGACGCCACGACAAACCGTAAGAGAGCTTGATAAATATATAGTGGGCCAAGAAAAAGCCAAAAAAGCAGT
GGCTGTGGCTTTGCGAAACAGGTACCGTCGCAGTAAGTTGAAAGAAGATTTGATTGATGAGGTTGTACCGAAGAATATTT
TAATGATTGGCCCAACGGGCGTAGGTAAAACCGAAATTGCCCGTCGTTTAGCTAAACTGGTAAAAGCCCCTTTTGTTAAA
ATTGAAGCCACTAAATTTACAGAAGTGGGCTATGTGGGGCGGGATGTAGAATCCATGGTCAGGGATTTGGTCGAAACCTC
CATTCGCATGCTTAAGCAAGAAAAAGTAGAAGAAGTGGAAGAACGGGCCAAGCGACTGGCTGATGAAAGAATTATTGAAC
TTTTAGCACCCTATCCGAACCGGCCTACCCAAACAAAAAATCCCTTTGAAATGCTATTTGGCGGGGTGCAGGATACTCAA
CAGGAAGATAAATCCTGGGAGCAGCAAAATAGTAGGATTAAGTTTGAGAGGGAAACTCTACGGGAAAAAATGTCCCGCTG
TGAGTTAGAAAAAGATTATCTAGAAATTGAAGTGGAAGATCAAAGCACACCTATGCTTGAGGTTTTTAGTAACAGTGGCG
TAGAAGAGATGGGCGTTAACATGCAAGACGTGTTAGGGAGTATTTTCCCAAGGAAAAAACGTAAACGCAAGGTCACTGTA
GAAGAGGCTCGCAAGATATTAACCCAGCAAGAAGCTCAGAAATTGATCGATATGGATGAATTGACTGCACAGGCGGTGAA
GCGTGCAGAAGATCATGGGATTATCTTCCTTGATGAGATTGATAAAATTGCTACAAGGGAAGGGACCACTGGTAGTGCAG
ATGTATCACGTGGAGGCGTACAAAGGGATATCCTTCCTATTGTGGAAGGATCCACAGTAATGACAAAGTATGGCCCTGTA
AAGACAGATCATATCCTTTTCATAGCAGCCGGGGCTTTCCATATAGCAAAACCCTCTGATTTAATTCCAGAGTTACAAGG
ACGTTTTCCCATCAGGGTAGAGTTAGAAAGCCTGAGCAGAAGTGATTTTGAACAAATTTTAACAGAGCCGCAAAACTCCT
TAATCAAACAATACACTGAGTTATTAGGGACTGAAGGACTTAAAATTGAATTTTCTAAAAATTCTCTTGTAGAAATTGCC
GATATTGCTTATACTGTAAATGAACAAAATGAAAACATTGGAGCAAGGCGGTTGCACACCATTCTCGAAAAATTATTGGA
GGATGTTTCCTTCGAGGCTCCAGAACTTAATGGACAGTCACTCACTATTGACAGTGATTATGTTCGTGAGAAGCTAAAGG
ATGTTGTAACAAATCGTGATCTTAGTCGTTATATTCTTTAA

Upstream 100 bases:

>100_bases
ACCTATGAGGCTCTAAATTTGGCCGCAGATATTTGTGTCTACACTAATAAAAATATTGTTGTTGAGGAAATTTAATTTAG
GTATCAAAGGAGTGGAGCCA

Downstream 100 bases:

>100_bases
AAAAACGGGGAGAGTGACAGAATGAAGACCTTATTGGAAAGAACACGTACAATTAATAAACTATTACAGAAATCTGCTGG
TTATCCTGTTGACTTTGGCG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MEALTPRQTVRELDKYIVGQEKAKKAVAVALRNRYRRSKLKEDLIDEVVPKNILMIGPTGVGKTEIARRLAKLVKAPFVK
IEATKFTEVGYVGRDVESMVRDLVETSIRMLKQEKVEEVEERAKRLADERIIELLAPYPNRPTQTKNPFEMLFGGVQDTQ
QEDKSWEQQNSRIKFERETLREKMSRCELEKDYLEIEVEDQSTPMLEVFSNSGVEEMGVNMQDVLGSIFPRKKRKRKVTV
EEARKILTQQEAQKLIDMDELTAQAVKRAEDHGIIFLDEIDKIATREGTTGSADVSRGGVQRDILPIVEGSTVMTKYGPV
KTDHILFIAAGAFHIAKPSDLIPELQGRFPIRVELESLSRSDFEQILTEPQNSLIKQYTELLGTEGLKIEFSKNSLVEIA
DIAYTVNEQNENIGARRLHTILEKLLEDVSFEAPELNGQSLTIDSDYVREKLKDVVTNRDLSRYIL

Sequences:

>Translated_466_residues
MEALTPRQTVRELDKYIVGQEKAKKAVAVALRNRYRRSKLKEDLIDEVVPKNILMIGPTGVGKTEIARRLAKLVKAPFVK
IEATKFTEVGYVGRDVESMVRDLVETSIRMLKQEKVEEVEERAKRLADERIIELLAPYPNRPTQTKNPFEMLFGGVQDTQ
QEDKSWEQQNSRIKFERETLREKMSRCELEKDYLEIEVEDQSTPMLEVFSNSGVEEMGVNMQDVLGSIFPRKKRKRKVTV
EEARKILTQQEAQKLIDMDELTAQAVKRAEDHGIIFLDEIDKIATREGTTGSADVSRGGVQRDILPIVEGSTVMTKYGPV
KTDHILFIAAGAFHIAKPSDLIPELQGRFPIRVELESLSRSDFEQILTEPQNSLIKQYTELLGTEGLKIEFSKNSLVEIA
DIAYTVNEQNENIGARRLHTILEKLLEDVSFEAPELNGQSLTIDSDYVREKLKDVVTNRDLSRYIL
>Mature_466_residues
MEALTPRQTVRELDKYIVGQEKAKKAVAVALRNRYRRSKLKEDLIDEVVPKNILMIGPTGVGKTEIARRLAKLVKAPFVK
IEATKFTEVGYVGRDVESMVRDLVETSIRMLKQEKVEEVEERAKRLADERIIELLAPYPNRPTQTKNPFEMLFGGVQDTQ
QEDKSWEQQNSRIKFERETLREKMSRCELEKDYLEIEVEDQSTPMLEVFSNSGVEEMGVNMQDVLGSIFPRKKRKRKVTV
EEARKILTQQEAQKLIDMDELTAQAVKRAEDHGIIFLDEIDKIATREGTTGSADVSRGGVQRDILPIVEGSTVMTKYGPV
KTDHILFIAAGAFHIAKPSDLIPELQGRFPIRVELESLSRSDFEQILTEPQNSLIKQYTELLGTEGLKIEFSKNSLVEIA
DIAYTVNEQNENIGARRLHTILEKLLEDVSFEAPELNGQSLTIDSDYVREKLKDVVTNRDLSRYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Homo sapiens, GI7242140, Length=111, Percent_Identity=36.036036036036, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790366, Length=467, Percent_Identity=52.03426124197, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1786642, Length=103, Percent_Identity=46.6019417475728, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982908, Length=244, Percent_Identity=31.1475409836066, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982905, Length=244, Percent_Identity=31.1475409836066, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319704, Length=141, Percent_Identity=32.6241134751773, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648291, Length=249, Percent_Identity=30.5220883534137, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648289, Length=249, Percent_Identity=30.5220883534137, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_DESRM (A4J5Z5)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113323.1
- ProteinModelPortal:   A4J5Z5
- SMR:   A4J5Z5
- STRING:   A4J5Z5
- GeneID:   4958498
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_1980
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- PhylomeDB:   A4J5Z5
- ProtClustDB:   PRK05201
- BioCyc:   DRED349161:DRED_1980-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 53181; Mature: 53181

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 278-278 BINDING 344-344 BINDING 416-416

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEALTPRQTVRELDKYIVGQEKAKKAVAVALRNRYRRSKLKEDLIDEVVPKNILMIGPTG
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
VGKTEIARRLAKLVKAPFVKIEATKFTEVGYVGRDVESMVRDLVETSIRMLKQEKVEEVE
CCHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERAKRLADERIIELLAPYPNRPTQTKNPFEMLFGGVQDTQQEDKSWEQQNSRIKFERETL
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCEEHHHHHH
REKMSRCELEKDYLEIEVEDQSTPMLEVFSNSGVEEMGVNMQDVLGSIFPRKKRKRKVTV
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCCHHHHCCCHHHHHHHHCCCHHHHCCCCH
EEARKILTQQEAQKLIDMDELTAQAVKRAEDHGIIFLDEIDKIATREGTTGSADVSRGGV
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCHHCCCC
QRDILPIVEGSTVMTKYGPVKTDHILFIAAGAFHIAKPSDLIPELQGRFPIRVELESLSR
CCCCHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEHHHCCH
SDFEQILTEPQNSLIKQYTELLGTEGLKIEFSKNSLVEIADIAYTVNEQNENIGARRLHT
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHEEECCCCCCCHHHHHHH
ILEKLLEDVSFEAPELNGQSLTIDSDYVREKLKDVVTNRDLSRYIL
HHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MEALTPRQTVRELDKYIVGQEKAKKAVAVALRNRYRRSKLKEDLIDEVVPKNILMIGPTG
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
VGKTEIARRLAKLVKAPFVKIEATKFTEVGYVGRDVESMVRDLVETSIRMLKQEKVEEVE
CCHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERAKRLADERIIELLAPYPNRPTQTKNPFEMLFGGVQDTQQEDKSWEQQNSRIKFERETL
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCEEHHHHHH
REKMSRCELEKDYLEIEVEDQSTPMLEVFSNSGVEEMGVNMQDVLGSIFPRKKRKRKVTV
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCCHHHHCCCHHHHHHHHCCCHHHHCCCCH
EEARKILTQQEAQKLIDMDELTAQAVKRAEDHGIIFLDEIDKIATREGTTGSADVSRGGV
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCHHCCCC
QRDILPIVEGSTVMTKYGPVKTDHILFIAAGAFHIAKPSDLIPELQGRFPIRVELESLSR
CCCCHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEHHHCCH
SDFEQILTEPQNSLIKQYTELLGTEGLKIEFSKNSLVEIADIAYTVNEQNENIGARRLHT
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHEEECCCCCCCHHHHHHH
ILEKLLEDVSFEAPELNGQSLTIDSDYVREKLKDVVTNRDLSRYIL
HHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA