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The map label for this gene is ispG [H]

Identifier: 134299815

GI number: 134299815

Start: 2163483

End: 2164532

Strand: Reverse

Name: ispG [H]

Synonym: Dred_1968

Alternate gene names: 134299815

Gene position: 2164532-2163483 (Counterclockwise)

Preceding gene: 134299816

Following gene: 134299814

Centisome position: 59.99

GC content: 44.67

Gene sequence:

>1050_bases
ATGAATTCTAAAATAACACGACCAATTATGGTGGGTAAAGTACAGATTGGCGGGGGTGGACCTGTTGTTGTACAATCCAT
GACAAATACGGATACCAGGAATGCCCTTGCTACAATTGATCAAATTAACGAGTTATCTGCGGTGGGCTGTGAAGTAATCA
GAGTAGCTGTGCCGGACAAAACAGCTGCCGAAGCTTTACCAACTATTAAAGCAGGTATTAGTATACCCCTAATTGCTGAT
ATTCACTTTGATTATCGACTGGCCCTATTGGCTATAGAGGCTGGTGTTGATGGTTTAAGAATCAACCCTGGCAATATAGG
TGGAAAGGAAAAAGTACAAGAAGTTGTAGCAGCGGCCCGGGAACGAAGAGTTCCAATACGCATTGGTGTAAATGCTGGTT
CCCTGGAAAGGGAATTACTAACTAAATATGGCGGAGTGACGCCGGATGCGATGGTGGAAAGTGCCTTAAACCATATTAGG
CTATTAGAGGAATTAAACTACAACGAAATAAAGGTGTCACTAAAAGCTTCCAATATTCCTCTTATGCTGGAAGCTTACCG
TAAACTATCTGCCAGGGTAGAATACCCAATGCATGTGGGTGTAACCGAAGCAGGTACGGTAAAAAGTGGTACCATAAAAT
CTGCTGTGGGAATCGGAGCCCTGCTTGCTGAAGGGATAGGTGATACCATTCGAGTATCTCTCACCGGTGATCCTAAATAC
GAAGTGAATGTAGCATGGGAAATATTAAAGGCCTTGGGACTACGCAGGCGGGGAGCTGAGCTGATTTCCTGCCCTACATG
TGGGAGAACGCAAATTAATCTGATAAAATTGGCTGAAGAGGTTGAGCAGAGAATAGCTACAGTGGACAAACCCATTAAAA
TTGCTGTGATGGGTTGTGCTGTCAATGGGCCCGGCGAAGCAAGGGAAGCAGATATAGGGATAGCAGGTGGCAAAGGGGTG
GGGCTAATCTTTCGCAAGGGAGAAATAATCCGACAGGTTCCCGAAGACCAACTGTTAGAAGAGTTGCTGAAAGAAATAGA
GAATCTTTAA

Upstream 100 bases:

>100_bases
TTCATCTTATCGGTTTTGGACTATTATTACTATTGATGGTGGTAATCACATATAACGATATTGTTAATTTAATGTTTGGT
AATCGTTAGCGGAGGATAAC

Downstream 100 bases:

>100_bases
CTATTCATGAACATCTATCTAATGTCGCAATATGACTATCTAAATATATCAGGAGGATCTTAATATGCGTGTTAGTCAAA
TGCTCGTTCCAACTTTAAGG

Product: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MNSKITRPIMVGKVQIGGGGPVVVQSMTNTDTRNALATIDQINELSAVGCEVIRVAVPDKTAAEALPTIKAGISIPLIAD
IHFDYRLALLAIEAGVDGLRINPGNIGGKEKVQEVVAAARERRVPIRIGVNAGSLERELLTKYGGVTPDAMVESALNHIR
LLEELNYNEIKVSLKASNIPLMLEAYRKLSARVEYPMHVGVTEAGTVKSGTIKSAVGIGALLAEGIGDTIRVSLTGDPKY
EVNVAWEILKALGLRRRGAELISCPTCGRTQINLIKLAEEVEQRIATVDKPIKIAVMGCAVNGPGEAREADIGIAGGKGV
GLIFRKGEIIRQVPEDQLLEELLKEIENL

Sequences:

>Translated_349_residues
MNSKITRPIMVGKVQIGGGGPVVVQSMTNTDTRNALATIDQINELSAVGCEVIRVAVPDKTAAEALPTIKAGISIPLIAD
IHFDYRLALLAIEAGVDGLRINPGNIGGKEKVQEVVAAARERRVPIRIGVNAGSLERELLTKYGGVTPDAMVESALNHIR
LLEELNYNEIKVSLKASNIPLMLEAYRKLSARVEYPMHVGVTEAGTVKSGTIKSAVGIGALLAEGIGDTIRVSLTGDPKY
EVNVAWEILKALGLRRRGAELISCPTCGRTQINLIKLAEEVEQRIATVDKPIKIAVMGCAVNGPGEAREADIGIAGGKGV
GLIFRKGEIIRQVPEDQLLEELLKEIENL
>Mature_349_residues
MNSKITRPIMVGKVQIGGGGPVVVQSMTNTDTRNALATIDQINELSAVGCEVIRVAVPDKTAAEALPTIKAGISIPLIAD
IHFDYRLALLAIEAGVDGLRINPGNIGGKEKVQEVVAAARERRVPIRIGVNAGSLERELLTKYGGVTPDAMVESALNHIR
LLEELNYNEIKVSLKASNIPLMLEAYRKLSARVEYPMHVGVTEAGTVKSGTIKSAVGIGALLAEGIGDTIRVSLTGDPKY
EVNVAWEILKALGLRRRGAELISCPTCGRTQINLIKLAEEVEQRIATVDKPIKIAVMGCAVNGPGEAREADIGIAGGKGV
GLIFRKGEIIRQVPEDQLLEELLKEIENL

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=339, Percent_Identity=48.6725663716814, Blast_Score=347, Evalue=7e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37283; Mature: 37283

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKITRPIMVGKVQIGGGGPVVVQSMTNTDTRNALATIDQINELSAVGCEVIRVAVPDK
CCCCCCCCEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCC
TAAEALPTIKAGISIPLIADIHFDYRLALLAIEAGVDGLRINPGNIGGKEKVQEVVAAAR
HHHHHCCHHHCCCCCEEEEEECCCEEEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHH
ERRVPIRIGVNAGSLERELLTKYGGVTPDAMVESALNHIRLLEELNYNEIKVSLKASNIP
HCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
LMLEAYRKLSARVEYPMHVGVTEAGTVKSGTIKSAVGIGALLAEGIGDTIRVSLTGDPKY
HHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCE
EVNVAWEILKALGLRRRGAELISCPTCGRTQINLIKLAEEVEQRIATVDKPIKIAVMGCA
EEHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
VNGPGEAREADIGIAGGKGVGLIFRKGEIIRQVPEDQLLEELLKEIENL
ECCCCCCCCCCEEEECCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSKITRPIMVGKVQIGGGGPVVVQSMTNTDTRNALATIDQINELSAVGCEVIRVAVPDK
CCCCCCCCEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCC
TAAEALPTIKAGISIPLIADIHFDYRLALLAIEAGVDGLRINPGNIGGKEKVQEVVAAAR
HHHHHCCHHHCCCCCEEEEEECCCEEEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHH
ERRVPIRIGVNAGSLERELLTKYGGVTPDAMVESALNHIRLLEELNYNEIKVSLKASNIP
HCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
LMLEAYRKLSARVEYPMHVGVTEAGTVKSGTIKSAVGIGALLAEGIGDTIRVSLTGDPKY
HHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCE
EVNVAWEILKALGLRRRGAELISCPTCGRTQINLIKLAEEVEQRIATVDKPIKIAVMGCA
EEHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
VNGPGEAREADIGIAGGKGVGLIFRKGEIIRQVPEDQLLEELLKEIENL
ECCCCCCCCCCEEEECCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA