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The map label for this gene is proS

Identifier: 134299814

GI number: 134299814

Start: 2161709

End: 2163418

Strand: Reverse

Name: proS

Synonym: Dred_1967

Alternate gene names: 134299814

Gene position: 2163418-2161709 (Counterclockwise)

Preceding gene: 134299815

Following gene: 134299813

Centisome position: 59.96

GC content: 45.38

Gene sequence:

>1710_bases
ATGCGTGTTAGTCAAATGCTCGTTCCAACTTTAAGGGAAGTTCCGGCAGAGGCCGAAGTGGTAAGTCACCAACTATTGCT
GAGAGCTGGTTTTATTCGTAAAACAGCCTCCGGTGTATATACATATCTGCCATTAGCCCAACGTGTTCTCAAAAAGTTAA
GGCAAATTATTCGGGAAGAAATAGACAAGCAGGGTGGGCAGGAATTGTTAATGCCCATCATTCAACCTGCGGAGATGTGG
TTAGAGTCCGGTCGCTGGCATGTGTACGGTCCGGAGTTGTTCCGTCTTAAAGATCGCCACGATCGTAATTTCTGTTTGGG
ACCCACCCATGAAGAAGTTATTACGATTTTAATACGTGGAGAAGTTCGTTCGTATAAACAAATGCCCTTGTTACTATACC
AGATTCAAAATAAATATAGGGATGAACGCAGACCTCGCTTTGGTTTGCTGCGGGGCAGAGAGTTTATTATGAAGGACTTG
TATTCCTTTGATAGAGACGAAGCAGGACTGGATATAAGTTATCAAAAGATGCATGAAGCATATACAAGCATATTTAGCCG
TTGTGGTGTGAAGTTTCGACCGGTTGAGGCTGATTCCGGTGCAATCGGCGGTAGTAGCACCCATGAGTTCATGGTGTTAG
CGGAATCAGGCGAAGCAGCTATTTTGTATTGCTCTGATTGTGATTATGCGGCCAATGTTGAGAAGGCAACAACACTGCCA
GCCGCTGGCCTTGATCCTGCAATTCAACAGCTAGAATTGCAGGAAGTAAGTACACCCGGTAAAAAAACTGCTGAGGAAGT
GGCAAAGTTCCTGGGTGTCGAGCCTTTCCAAGTGATTAAGACTATGTTTTATAAAACTGATAAAGAAGTTGTAGCAGCAC
TGGTTCGTGGTGATCGTGATGTAAACGAAATTAAACTGTTAAATGTCCTTGGGGCGTTGACCCTCGATCTGGCCGATGAA
AGCACGGTTCAGCAAGTGACAGGTGCTCCCACTGGTTATGTGGGTCCTGTGGGACTGGAGAACATTCGTATTGTAGCTGA
CATGGAAGTAATGGCCCTGGTTAACGCCGTTGTAGGGGCCAATAAACAGGATGCTCACCTGATTAATGTTAACCCTAAGC
GGGACTTCCAGCCAGAAATCGTAGAAGATATTCGTATGGTTAAGGCAGGAGAGCCTTGCCCTAAGTGTGGCGCACAGCTA
CTGGAGGCCCGGGGCATTGAAGTTGGTCAAATCTTTAAATTAGGTACTAAATACAGTAAAGCTTTAGGGGCTACTTTTCT
GGATGAAAACGGTAAGGAACAGCCCATTGTTATGGGCTGCTACGGCATTGGGGTAAGTCGTACTATGGCAGCAGCCATTG
AGCAAAACTATGACAAAGATGGCATTATCTGGCCTGCTAGCATTGCACCATACCAGGCCATTGTTATTCCTGTAGCTCCG
AAAGACGATAACCAGATGAAAATTGCTGAGGAACTTTACAAAGCACTTAATCAAGCTGGTGTAGAAACCATTCTGGACGA
CCGTTCCGAACGCCCCGGAGTGAAATTTAAAGATGCTGACCTAATCGGCTACCCATTAAGAATTGTGGTTGGTAACAAAG
CTGTTACCGAAGGCGTAGTGGAAATTCGACAGCGCCGTTCCGGGCAAACAGACCTAGTTTCTGTTAAAGATTCTGTGCCT
AAGATATTAGAAATTTTACCAACACTGTAG

Upstream 100 bases:

>100_bases
TTAGAAGAGTTGCTGAAAGAAATAGAGAATCTTTAACTATTCATGAACATCTATCTAATGTCGCAATATGACTATCTAAA
TATATCAGGAGGATCTTAAT

Downstream 100 bases:

>100_bases
GTGCATATATAAGATGCTAAACAAAATACTGGTACTGTCGGTCTCTGCCGGTGAAGGGCATATGAGGGCAGCAGCGGCTG
TCAAGGAAGAAATTATTCGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MRVSQMLVPTLREVPAEAEVVSHQLLLRAGFIRKTASGVYTYLPLAQRVLKKLRQIIREEIDKQGGQELLMPIIQPAEMW
LESGRWHVYGPELFRLKDRHDRNFCLGPTHEEVITILIRGEVRSYKQMPLLLYQIQNKYRDERRPRFGLLRGREFIMKDL
YSFDRDEAGLDISYQKMHEAYTSIFSRCGVKFRPVEADSGAIGGSSTHEFMVLAESGEAAILYCSDCDYAANVEKATTLP
AAGLDPAIQQLELQEVSTPGKKTAEEVAKFLGVEPFQVIKTMFYKTDKEVVAALVRGDRDVNEIKLLNVLGALTLDLADE
STVQQVTGAPTGYVGPVGLENIRIVADMEVMALVNAVVGANKQDAHLINVNPKRDFQPEIVEDIRMVKAGEPCPKCGAQL
LEARGIEVGQIFKLGTKYSKALGATFLDENGKEQPIVMGCYGIGVSRTMAAAIEQNYDKDGIIWPASIAPYQAIVIPVAP
KDDNQMKIAEELYKALNQAGVETILDDRSERPGVKFKDADLIGYPLRIVVGNKAVTEGVVEIRQRRSGQTDLVSVKDSVP
KILEILPTL

Sequences:

>Translated_569_residues
MRVSQMLVPTLREVPAEAEVVSHQLLLRAGFIRKTASGVYTYLPLAQRVLKKLRQIIREEIDKQGGQELLMPIIQPAEMW
LESGRWHVYGPELFRLKDRHDRNFCLGPTHEEVITILIRGEVRSYKQMPLLLYQIQNKYRDERRPRFGLLRGREFIMKDL
YSFDRDEAGLDISYQKMHEAYTSIFSRCGVKFRPVEADSGAIGGSSTHEFMVLAESGEAAILYCSDCDYAANVEKATTLP
AAGLDPAIQQLELQEVSTPGKKTAEEVAKFLGVEPFQVIKTMFYKTDKEVVAALVRGDRDVNEIKLLNVLGALTLDLADE
STVQQVTGAPTGYVGPVGLENIRIVADMEVMALVNAVVGANKQDAHLINVNPKRDFQPEIVEDIRMVKAGEPCPKCGAQL
LEARGIEVGQIFKLGTKYSKALGATFLDENGKEQPIVMGCYGIGVSRTMAAAIEQNYDKDGIIWPASIAPYQAIVIPVAP
KDDNQMKIAEELYKALNQAGVETILDDRSERPGVKFKDADLIGYPLRIVVGNKAVTEGVVEIRQRRSGQTDLVSVKDSVP
KILEILPTL
>Mature_569_residues
MRVSQMLVPTLREVPAEAEVVSHQLLLRAGFIRKTASGVYTYLPLAQRVLKKLRQIIREEIDKQGGQELLMPIIQPAEMW
LESGRWHVYGPELFRLKDRHDRNFCLGPTHEEVITILIRGEVRSYKQMPLLLYQIQNKYRDERRPRFGLLRGREFIMKDL
YSFDRDEAGLDISYQKMHEAYTSIFSRCGVKFRPVEADSGAIGGSSTHEFMVLAESGEAAILYCSDCDYAANVEKATTLP
AAGLDPAIQQLELQEVSTPGKKTAEEVAKFLGVEPFQVIKTMFYKTDKEVVAALVRGDRDVNEIKLLNVLGALTLDLADE
STVQQVTGAPTGYVGPVGLENIRIVADMEVMALVNAVVGANKQDAHLINVNPKRDFQPEIVEDIRMVKAGEPCPKCGAQL
LEARGIEVGQIFKLGTKYSKALGATFLDENGKEQPIVMGCYGIGVSRTMAAAIEQNYDKDGIIWPASIAPYQAIVIPVAP
KDDNQMKIAEELYKALNQAGVETILDDRSERPGVKFKDADLIGYPLRIVVGNKAVTEGVVEIRQRRSGQTDLVSVKDSVP
KILEILPTL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=228, Percent_Identity=44.7368421052632, Blast_Score=219, Evalue=7e-57,
Organism=Escherichia coli, GI1786392, Length=568, Percent_Identity=49.2957746478873, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI115532348, Length=215, Percent_Identity=40, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=90, Percent_Identity=38.8888888888889, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=557, Percent_Identity=32.3159784560144, Blast_Score=262, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24656200, Length=236, Percent_Identity=36.4406779661017, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_DESRM (A4J5Y2)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113310.1
- ProteinModelPortal:   A4J5Y2
- SMR:   A4J5Y2
- STRING:   A4J5Y2
- GeneID:   4956707
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_1967
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A4J5Y2
- ProtClustDB:   PRK09194
- BioCyc:   DRED349161:DRED_1967-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63261; Mature: 63261

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSQMLVPTLREVPAEAEVVSHQLLLRAGFIRKTASGVYTYLPLAQRVLKKLRQIIREE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IDKQGGQELLMPIIQPAEMWLESGRWHVYGPELFRLKDRHDRNFCLGPTHEEVITILIRG
HHHCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHEECCCCCCCCCCCCCHHHHEEEEEEC
EVRSYKQMPLLLYQIQNKYRDERRPRFGLLRGREFIMKDLYSFDRDEAGLDISYQKMHEA
CHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
YTSIFSRCGVKFRPVEADSGAIGGSSTHEFMVLAESGEAAILYCSDCDYAANVEKATTLP
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCHHCCCC
AAGLDPAIQQLELQEVSTPGKKTAEEVAKFLGVEPFQVIKTMFYKTDKEVVAALVRGDRD
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCC
VNEIKLLNVLGALTLDLADESTVQQVTGAPTGYVGPVGLENIRIVADMEVMALVNAVVGA
HHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHCC
NKQDAHLINVNPKRDFQPEIVEDIRMVKAGEPCPKCGAQLLEARGIEVGQIFKLGTKYSK
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
ALGATFLDENGKEQPIVMGCYGIGVSRTMAAAIEQNYDKDGIIWPASIAPYQAIVIPVAP
HHCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECC
KDDNQMKIAEELYKALNQAGVETILDDRSERPGVKFKDADLIGYPLRIVVGNKAVTEGVV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEECCCEEEEECCHHHHHHHH
EIRQRRSGQTDLVSVKDSVPKILEILPTL
HHHHHCCCCCCEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVSQMLVPTLREVPAEAEVVSHQLLLRAGFIRKTASGVYTYLPLAQRVLKKLRQIIREE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IDKQGGQELLMPIIQPAEMWLESGRWHVYGPELFRLKDRHDRNFCLGPTHEEVITILIRG
HHHCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHEECCCCCCCCCCCCCHHHHEEEEEEC
EVRSYKQMPLLLYQIQNKYRDERRPRFGLLRGREFIMKDLYSFDRDEAGLDISYQKMHEA
CHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
YTSIFSRCGVKFRPVEADSGAIGGSSTHEFMVLAESGEAAILYCSDCDYAANVEKATTLP
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCHHCCCC
AAGLDPAIQQLELQEVSTPGKKTAEEVAKFLGVEPFQVIKTMFYKTDKEVVAALVRGDRD
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCC
VNEIKLLNVLGALTLDLADESTVQQVTGAPTGYVGPVGLENIRIVADMEVMALVNAVVGA
HHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHCC
NKQDAHLINVNPKRDFQPEIVEDIRMVKAGEPCPKCGAQLLEARGIEVGQIFKLGTKYSK
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
ALGATFLDENGKEQPIVMGCYGIGVSRTMAAAIEQNYDKDGIIWPASIAPYQAIVIPVAP
HHCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECC
KDDNQMKIAEELYKALNQAGVETILDDRSERPGVKFKDADLIGYPLRIVVGNKAVTEGVV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEECCCEEEEECCHHHHHHHH
EIRQRRSGQTDLVSVKDSVPKILEILPTL
HHHHHCCCCCCEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA