Click here to switch to the map view.

The map label for this gene is mdh

Identifier: 134299720

GI number: 134299720

Start: 2052909

End: 2053835

Strand: Reverse

Name: mdh

Synonym: Dred_1867

Alternate gene names: 134299720

Gene position: 2053835-2052909 (Counterclockwise)

Preceding gene: 134299721

Following gene: 134299719

Centisome position: 56.92

GC content: 44.23

Gene sequence:

>927_bases
ATGAAAAGGAAGAAAATTACTATTGTGGGTGCAGGGAATGTAGGGGCAACCTGTGCTCATTGGGCTGCGGCAAAGGAATT
AGGAGATATCGTACTTATCGATGTGGCTGAAGGGATACCCCAGGGAAAAGCGCTGGACTTAATGGAAGCAGCTCCAGTGG
AAGGGTATGACAGCGTGATAATAGGTACCAATGATTATGCAGATACCGCTGATTCCGATGTTGTTGTGATTACCGCCGGA
GTAGCTCGAAAACCGGGCATGAGTCGGGACGATTTATTGAATATCAATGCCGGAATTGTAAGAAAGGTCACTGAAGAAAT
TGTTAAGTATTCTCCCAATGCCTATCTACTGATAGTGACAAACCCTGTGGATGTAAGCGCATATATCGCTTATAAAGCCA
GTGGCTTTTCAGCAAACCGCGTTTTTGGTTTATCCGGGGTTCTTGATTCAGCTCGTTTTCGCACTTTCATAGCCAGAGAA
TTAAATGTTTCCTTTGAAGATGTAATATCCTTTGTTTTAGGTGGTCATGGTGATGATATGGTTCCCATGGTTCGCTACAC
CTATGTGGGCGGTATCCCCGTTGAAAAACTGATTCCCACTGATCGTTTAGAAGCAATGGTAGAACGGGCCAGGAAGGGCG
GGGCGGAAATCGTTAACTATCTAAAGACTGGAAGTGCCTATTATGCTCCCAGTGCATCGGTTATGCAAATGGCAGAGTGT
ATTCTAAAAGATAAGAAGCGCATTCTTCCTGTTTCTGCTTTCTTACAAGGAGAGTACGGCGAATCAGATGTTTTCTCTGG
TGTGCCGGCTATCATAGGCGGCGATGGTGTGGAGAAGATTATTGAGCTTGATCTGGACGATGAGGAAATGGCTGCCCTTA
GAAAATCCATTAATTCTGTAAGAAACAATATTTCTAAATTAGGATGA

Upstream 100 bases:

>100_bases
ACATGGTATAAAAAGATGGTTAGGCAGGTAAATAATAGAATAAAAGGATTTTAAGTTCACTATCCTATGTGAAATTATTC
AATACAGGAGGAGTAGCAAG

Downstream 100 bases:

>100_bases
TAAAAAACGTTAGGTTTCTAAACGCTTGAGTCTAACTCAACCTATATAAAGACAGTTCGAGGAATTACTATTAATTTAAC
CGATTTAAGAAATAACGAAT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKRKKITIVGAGNVGATCAHWAAAKELGDIVLIDVAEGIPQGKALDLMEAAPVEGYDSVIIGTNDYADTADSDVVVITAG
VARKPGMSRDDLLNINAGIVRKVTEEIVKYSPNAYLLIVTNPVDVSAYIAYKASGFSANRVFGLSGVLDSARFRTFIARE
LNVSFEDVISFVLGGHGDDMVPMVRYTYVGGIPVEKLIPTDRLEAMVERARKGGAEIVNYLKTGSAYYAPSASVMQMAEC
ILKDKKRILPVSAFLQGEYGESDVFSGVPAIIGGDGVEKIIELDLDDEEMAALRKSINSVRNNISKLG

Sequences:

>Translated_308_residues
MKRKKITIVGAGNVGATCAHWAAAKELGDIVLIDVAEGIPQGKALDLMEAAPVEGYDSVIIGTNDYADTADSDVVVITAG
VARKPGMSRDDLLNINAGIVRKVTEEIVKYSPNAYLLIVTNPVDVSAYIAYKASGFSANRVFGLSGVLDSARFRTFIARE
LNVSFEDVISFVLGGHGDDMVPMVRYTYVGGIPVEKLIPTDRLEAMVERARKGGAEIVNYLKTGSAYYAPSASVMQMAEC
ILKDKKRILPVSAFLQGEYGESDVFSGVPAIIGGDGVEKIIELDLDDEEMAALRKSINSVRNNISKLG
>Mature_308_residues
MKRKKITIVGAGNVGATCAHWAAAKELGDIVLIDVAEGIPQGKALDLMEAAPVEGYDSVIIGTNDYADTADSDVVVITAG
VARKPGMSRDDLLNINAGIVRKVTEEIVKYSPNAYLLIVTNPVDVSAYIAYKASGFSANRVFGLSGVLDSARFRTFIARE
LNVSFEDVISFVLGGHGDDMVPMVRYTYVGGIPVEKLIPTDRLEAMVERARKGGAEIVNYLKTGSAYYAPSASVMQMAEC
ILKDKKRILPVSAFLQGEYGESDVFSGVPAIIGGDGVEKIIELDLDDEEMAALRKSINSVRNNISKLG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=40.327868852459, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=40.327868852459, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI5031857, Length=307, Percent_Identity=37.785016286645, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI260099723, Length=307, Percent_Identity=37.785016286645, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI15082234, Length=316, Percent_Identity=35.126582278481, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=34.0983606557377, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=34.0983606557377, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI9257228, Length=307, Percent_Identity=33.5504885993485, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI4504973, Length=307, Percent_Identity=33.5504885993485, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI260099725, Length=213, Percent_Identity=39.906103286385, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI260099727, Length=198, Percent_Identity=41.4141414141414, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI207028494, Length=191, Percent_Identity=36.1256544502618, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI103472011, Length=306, Percent_Identity=29.0849673202614, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI21735621, Length=265, Percent_Identity=31.6981132075472, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI103472015, Length=178, Percent_Identity=32.0224719101124, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789632, Length=316, Percent_Identity=28.7974683544304, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=299, Percent_Identity=29.4314381270903, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17554310, Length=253, Percent_Identity=32.8063241106719, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322765, Length=220, Percent_Identity=33.1818181818182, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320125, Length=277, Percent_Identity=31.7689530685921, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324446, Length=170, Percent_Identity=31.7647058823529, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136226, Length=304, Percent_Identity=31.25, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24647881, Length=315, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI45550422, Length=305, Percent_Identity=27.2131147540984, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24663599, Length=257, Percent_Identity=33.4630350194552, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24663595, Length=244, Percent_Identity=30.7377049180328, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_DESRM (A4J5N8)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113216.1
- ProteinModelPortal:   A4J5N8
- SMR:   A4J5N8
- STRING:   A4J5N8
- GeneID:   4955588
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_1867
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- PhylomeDB:   A4J5N8
- ProtClustDB:   PRK06223
- BioCyc:   DRED349161:DRED_1867-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33015; Mature: 33015

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKKITIVGAGNVGATCAHWAAAKELGDIVLIDVAEGIPQGKALDLMEAAPVEGYDSVI
CCCEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHCCCCCCCCEEE
IGTNDYADTADSDVVVITAGVARKPGMSRDDLLNINAGIVRKVTEEIVKYSPNAYLLIVT
ECCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEE
NPVDVSAYIAYKASGFSANRVFGLSGVLDSARFRTFIARELNVSFEDVISFVLGGHGDDM
CCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
VPMVRYTYVGGIPVEKLIPTDRLEAMVERARKGGAEIVNYLKTGSAYYAPSASVMQMAEC
CHHHHEEEECCCCHHHHCCHHHHHHHHHHHHHCHHHHHHHHHCCCEEECCCHHHHHHHHH
ILKDKKRILPVSAFLQGEYGESDVFSGVPAIIGGDGVEKIIELDLDDEEMAALRKSINSV
HHHHHHHEECHHHHHCCCCCCCHHHCCCCCEECCCCHHHHEECCCCHHHHHHHHHHHHHH
RNNISKLG
HHHHHHCC
>Mature Secondary Structure
MKRKKITIVGAGNVGATCAHWAAAKELGDIVLIDVAEGIPQGKALDLMEAAPVEGYDSVI
CCCEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHCCCCCCCCEEE
IGTNDYADTADSDVVVITAGVARKPGMSRDDLLNINAGIVRKVTEEIVKYSPNAYLLIVT
ECCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEE
NPVDVSAYIAYKASGFSANRVFGLSGVLDSARFRTFIARELNVSFEDVISFVLGGHGDDM
CCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
VPMVRYTYVGGIPVEKLIPTDRLEAMVERARKGGAEIVNYLKTGSAYYAPSASVMQMAEC
CHHHHEEEECCCCHHHHCCHHHHHHHHHHHHHCHHHHHHHHHCCCEEECCCHHHHHHHHH
ILKDKKRILPVSAFLQGEYGESDVFSGVPAIIGGDGVEKIIELDLDDEEMAALRKSINSV
HHHHHHHEECHHHHHCCCCCCCHHHCCCCCEECCCCHHHHEECCCCHHHHHHHHHHHHHH
RNNISKLG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA