Click here to switch to the map view.

The map label for this gene is polA [H]

Identifier: 134299456

GI number: 134299456

Start: 1737914

End: 1740565

Strand: Reverse

Name: polA [H]

Synonym: Dred_1599

Alternate gene names: 134299456

Gene position: 1740565-1737914 (Counterclockwise)

Preceding gene: 134299459

Following gene: 134299455

Centisome position: 48.24

GC content: 46.27

Gene sequence:

>2652_bases
TTGTCCCTGGATACCATCATTATTATCGACGGCAACAGCCTAATTCATAGAGCTTTTCATGCCATCCCCCTGCTTTCCAA
CAGTAAAGGGGTTATTACCAACGCTGTATATGGTTTCACTAATATGGTTTTAAAGGTTATCAAAGAGCAGGAGCCGGGTC
TTGTTGCTGTTGCCTTTGATAAAGGCAAAACCACGTTCCGAAACCAGGAATATGCCGCATATAAAGGTACCCGTAAAGCT
ACCCCCGATGAACTGCGCCCTCAATTTATCCTGGCCAGGGATATCTTGGGAGCGATGCGAATACCCTTCTTTGAATTGGA
TGGCTTTGAGGCCGATGATTTAATCGGTACCATTGCTTGCCAGGCCGAGGCCACTGGACTCAACGTGTTAATTCTTACCG
GTGATAGGGACGCTTTGCAACTGGTGTCTCCTAAAACCAAGGTTATGCTGACCCGTAAAGGGATCACAGAAATTGACTTA
TTTGATGAAGGTAAGGTCTGGGACAAGTATGGTGTTACTCCAAGTCAGGTTGTGGATTTAAAAGGTCTGCAAGGGGACAC
CTCGGATAATATCCCCGGCGTTGCCGGTATCGGAGAAAAAACCGCCAGTGTATTAATTAAAGAATACGGATCTGTGGAAG
AAATCTTAAAAAACATTGATAAGCTCTCCCCCAGGCACCAGAAACTCTTACGAGGAAATGAAGAGATTGCACTTCTTTCA
AAGAAACTGGCCACTATTATCCGGGACGTCCCCATGGAACTGGATTTATCTGAATGTTCCTGGCACGGACCCGATAACCA
AAAGCTTCTGGAAATAGCCAAGGAGCTTGAATTTAAAACCCTGATTAAGCAACTTACCTCCGGTCAAAAATTTATAAAAC
AAAGCCAAGACAGTGGAACATCTGCTGATTTGCCACCTTTAGAAACCTATCAAGTGGGTTATCTAAATGTAACGGATCAA
GATGCCCTTGATGAACTTCTCATTGAGGTTAGTCGCCAGGGAAAAGTTGCCATTTATCTGGAAGGTTTGAGAAATCAAGG
GATTGACACTGCCTATTTGGCAATGGAGGATCACGATATATACAGATTGGCTATCCAAAACAAAGACAGTGACTCGGACC
CCTGCCTGACTGTGCTGAAGGCCATTTGTGAATGTGAGAGTGTCAAAAAATATTTTCACGATGCCAAGCAGGCCCTGTGG
ATGTTGCACCACCATAGCATTGAACTGAAGGCATTGGCCGCCGACACCATGGTATCGGCCTACCTGCTAAATCCTACGGC
CTCCAACTACGATATTAATGATGTGGCCTTAGAGCATCTGGGCATGGTATTGCCTGCGGAGGGCGAGACGGCAATGGCCA
GCAGAGCTGAGGCTATCCGCCGGTTGGCCCAGAAACTATTTGAAAAGTTGGCTGAGCGTCAGGAAGACAGATTGCTAACA
CAGGTGGAGTTGCCTCTGGTACGCATTCTGTCTGAGATGGAAATTGCCGGCGTAGCCGTTGATAAGCAGGGTTTACAGGA
CATGTCTGTGGAACTTCAAACTGTAACAGAAACCCTGACGACCAAAATCCACGAACTGGCCGGTGAGAACTTTAACATTA
ATTCCCCCAAACAATTGGGGCCCATTCTCTTTGAGAAGCTTAAGCTACCTGTACTTAAAAAAACCAAAACGGGGTATTCC
ACCGACGCAGAGGTTCTGGAGAAACTGGCCGAGGAGCACGAGATAGTCGCCTTAATTCTGGAATATCGGCAAATGGCCAA
ATTAAAATCCACCTATACCGATGGTCTTGCGGCCCTGGTGGACCCTCAAACAGGGCGGCTGCATTCAACCTTTCACCAAA
CGGTTACCGCCACTGGACGGTTATCAAGTGCAGAACCAAATTTACAAAACATTCCCATCCGGTTGGAAACCGGCAGGAAA
ATTCGCAAGGTGTTTATTCCCCGTCAAAGGGGTAATCTAATCCTGACAGCGGATTATTCTCAAATCGAATTGAGAGTGCT
TGCCCATATGTCCCAAGACGAAAGCTTCTTGGATGCATTTCGGCAGGGTCAAGATATCCATACCCGAACTGCTTCGGAGG
TTTTTGGCGTACCCATGGATCAGGTGACCTCGGAAATGCGAAGTCGGGCCAAGGCTGTCAACTTCGGTATCGTTTATGGC
ATCAGTGACTTTGGACTAGCTCGGGATTTAAAGGTGTCACGCAAAGAGGCCCAGGGTTATATCGATCATTACTTTGCACG
CTGCCCGGGGGTTCGGCAATATATTGACAGGGTTATCAAAGAGGCAAAAGAAACCGGCTATGTTAACACATTGTTAAACC
GCCGCCGTTACCTGCCGGAATTATTCAGCAAAAACTTCAATATTCGTAACTTCGGGGAACGAGCTGCCATGAATACTCCT
ATACAGGGCACTGCTGCGGATATCATAAAACTGGCCATGGTTAAAATCAGCCAGCAGCTCAAAGAGCAAAATCTTTCTTC
TCAAATGGTCCTACAAGTTCATGACGAATTAATTTTTGATGCACCGGAAAACGAAATCGAGTCATTGATAATGCTGGTTC
GCCAAAGTATGGAAAACGCATTACAATTAGACGTACCACTAGTGGTGGACATTAAGCTTGGTCCCAATTGGTACGAAGCT
AAGCCCATTTAA

Upstream 100 bases:

>100_bases
CTTCCCCAATTGATTATGAAGACAAAATTAACTCCCATGAAGGTATTTTGAGCCTTGCCGAGGAAAATACTAAAGATGCT
AAATAAGGAAGGTGCTTACT

Downstream 100 bases:

>100_bases
TCCGTCTTCTGAGAGGTGAAATCATGCCTGAACTACCGGAAGTAGAAACCATCGTTCGCTCCCTGGAAAAACATTTATCC
GGCCTTGTCATAACCTCCGT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 883; Mature: 882

Protein sequence:

>883_residues
MSLDTIIIIDGNSLIHRAFHAIPLLSNSKGVITNAVYGFTNMVLKVIKEQEPGLVAVAFDKGKTTFRNQEYAAYKGTRKA
TPDELRPQFILARDILGAMRIPFFELDGFEADDLIGTIACQAEATGLNVLILTGDRDALQLVSPKTKVMLTRKGITEIDL
FDEGKVWDKYGVTPSQVVDLKGLQGDTSDNIPGVAGIGEKTASVLIKEYGSVEEILKNIDKLSPRHQKLLRGNEEIALLS
KKLATIIRDVPMELDLSECSWHGPDNQKLLEIAKELEFKTLIKQLTSGQKFIKQSQDSGTSADLPPLETYQVGYLNVTDQ
DALDELLIEVSRQGKVAIYLEGLRNQGIDTAYLAMEDHDIYRLAIQNKDSDSDPCLTVLKAICECESVKKYFHDAKQALW
MLHHHSIELKALAADTMVSAYLLNPTASNYDINDVALEHLGMVLPAEGETAMASRAEAIRRLAQKLFEKLAERQEDRLLT
QVELPLVRILSEMEIAGVAVDKQGLQDMSVELQTVTETLTTKIHELAGENFNINSPKQLGPILFEKLKLPVLKKTKTGYS
TDAEVLEKLAEEHEIVALILEYRQMAKLKSTYTDGLAALVDPQTGRLHSTFHQTVTATGRLSSAEPNLQNIPIRLETGRK
IRKVFIPRQRGNLILTADYSQIELRVLAHMSQDESFLDAFRQGQDIHTRTASEVFGVPMDQVTSEMRSRAKAVNFGIVYG
ISDFGLARDLKVSRKEAQGYIDHYFARCPGVRQYIDRVIKEAKETGYVNTLLNRRRYLPELFSKNFNIRNFGERAAMNTP
IQGTAADIIKLAMVKISQQLKEQNLSSQMVLQVHDELIFDAPENEIESLIMLVRQSMENALQLDVPLVVDIKLGPNWYEA
KPI

Sequences:

>Translated_883_residues
MSLDTIIIIDGNSLIHRAFHAIPLLSNSKGVITNAVYGFTNMVLKVIKEQEPGLVAVAFDKGKTTFRNQEYAAYKGTRKA
TPDELRPQFILARDILGAMRIPFFELDGFEADDLIGTIACQAEATGLNVLILTGDRDALQLVSPKTKVMLTRKGITEIDL
FDEGKVWDKYGVTPSQVVDLKGLQGDTSDNIPGVAGIGEKTASVLIKEYGSVEEILKNIDKLSPRHQKLLRGNEEIALLS
KKLATIIRDVPMELDLSECSWHGPDNQKLLEIAKELEFKTLIKQLTSGQKFIKQSQDSGTSADLPPLETYQVGYLNVTDQ
DALDELLIEVSRQGKVAIYLEGLRNQGIDTAYLAMEDHDIYRLAIQNKDSDSDPCLTVLKAICECESVKKYFHDAKQALW
MLHHHSIELKALAADTMVSAYLLNPTASNYDINDVALEHLGMVLPAEGETAMASRAEAIRRLAQKLFEKLAERQEDRLLT
QVELPLVRILSEMEIAGVAVDKQGLQDMSVELQTVTETLTTKIHELAGENFNINSPKQLGPILFEKLKLPVLKKTKTGYS
TDAEVLEKLAEEHEIVALILEYRQMAKLKSTYTDGLAALVDPQTGRLHSTFHQTVTATGRLSSAEPNLQNIPIRLETGRK
IRKVFIPRQRGNLILTADYSQIELRVLAHMSQDESFLDAFRQGQDIHTRTASEVFGVPMDQVTSEMRSRAKAVNFGIVYG
ISDFGLARDLKVSRKEAQGYIDHYFARCPGVRQYIDRVIKEAKETGYVNTLLNRRRYLPELFSKNFNIRNFGERAAMNTP
IQGTAADIIKLAMVKISQQLKEQNLSSQMVLQVHDELIFDAPENEIESLIMLVRQSMENALQLDVPLVVDIKLGPNWYEA
KPI
>Mature_882_residues
SLDTIIIIDGNSLIHRAFHAIPLLSNSKGVITNAVYGFTNMVLKVIKEQEPGLVAVAFDKGKTTFRNQEYAAYKGTRKAT
PDELRPQFILARDILGAMRIPFFELDGFEADDLIGTIACQAEATGLNVLILTGDRDALQLVSPKTKVMLTRKGITEIDLF
DEGKVWDKYGVTPSQVVDLKGLQGDTSDNIPGVAGIGEKTASVLIKEYGSVEEILKNIDKLSPRHQKLLRGNEEIALLSK
KLATIIRDVPMELDLSECSWHGPDNQKLLEIAKELEFKTLIKQLTSGQKFIKQSQDSGTSADLPPLETYQVGYLNVTDQD
ALDELLIEVSRQGKVAIYLEGLRNQGIDTAYLAMEDHDIYRLAIQNKDSDSDPCLTVLKAICECESVKKYFHDAKQALWM
LHHHSIELKALAADTMVSAYLLNPTASNYDINDVALEHLGMVLPAEGETAMASRAEAIRRLAQKLFEKLAERQEDRLLTQ
VELPLVRILSEMEIAGVAVDKQGLQDMSVELQTVTETLTTKIHELAGENFNINSPKQLGPILFEKLKLPVLKKTKTGYST
DAEVLEKLAEEHEIVALILEYRQMAKLKSTYTDGLAALVDPQTGRLHSTFHQTVTATGRLSSAEPNLQNIPIRLETGRKI
RKVFIPRQRGNLILTADYSQIELRVLAHMSQDESFLDAFRQGQDIHTRTASEVFGVPMDQVTSEMRSRAKAVNFGIVYGI
SDFGLARDLKVSRKEAQGYIDHYFARCPGVRQYIDRVIKEAKETGYVNTLLNRRRYLPELFSKNFNIRNFGERAAMNTPI
QGTAADIIKLAMVKISQQLKEQNLSSQMVLQVHDELIFDAPENEIESLIMLVRQSMENALQLDVPLVVDIKLGPNWYEAK
PI

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=514, Percent_Identity=32.4902723735409, Blast_Score=249, Evalue=1e-65,
Organism=Homo sapiens, GI139394648, Length=654, Percent_Identity=26.4525993883792, Blast_Score=211, Evalue=4e-54,
Organism=Escherichia coli, GI1790294, Length=926, Percent_Identity=40.1727861771058, Blast_Score=616, Evalue=1e-177,
Organism=Escherichia coli, GI226510970, Length=254, Percent_Identity=27.9527559055118, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI86563272, Length=409, Percent_Identity=31.2958435207824, Blast_Score=184, Evalue=3e-46,
Organism=Drosophila melanogaster, GI17933644, Length=426, Percent_Identity=33.5680751173709, Blast_Score=236, Evalue=7e-62,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 98788; Mature: 98657

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDTIIIIDGNSLIHRAFHAIPLLSNSKGVITNAVYGFTNMVLKVIKEQEPGLVAVAFD
CCCCEEEEECCCHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
KGKTTFRNQEYAAYKGTRKATPDELRPQFILARDILGAMRIPFFELDGFEADDLIGTIAC
CCCCCCCCCCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHCCCEEECCCCCHHHHHEEEEE
QAEATGLNVLILTGDRDALQLVSPKTKVMLTRKGITEIDLFDEGKVWDKYGVTPSQVVDL
EEECCCCEEEEEECCCCHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHEEEC
KGLQGDTSDNIPGVAGIGEKTASVLIKEYGSVEEILKNIDKLSPRHQKLLRGNEEIALLS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHH
KKLATIIRDVPMELDLSECSWHGPDNQKLLEIAKELEFKTLIKQLTSGQKFIKQSQDSGT
HHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
SADLPPLETYQVGYLNVTDQDALDELLIEVSRQGKVAIYLEGLRNQGIDTAYLAMEDHDI
CCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCE
YRLAIQNKDSDSDPCLTVLKAICECESVKKYFHDAKQALWMLHHHSIELKALAADTMVSA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
YLLNPTASNYDINDVALEHLGMVLPAEGETAMASRAEAIRRLAQKLFEKLAERQEDRLLT
HEECCCCCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVELPLVRILSEMEIAGVAVDKQGLQDMSVELQTVTETLTTKIHELAGENFNINSPKQLG
HHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
PILFEKLKLPVLKKTKTGYSTDAEVLEKLAEEHEIVALILEYRQMAKLKSTYTDGLAALV
HHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
DPQTGRLHSTFHQTVTATGRLSSAEPNLQNIPIRLETGRKIRKVFIPRQRGNLILTADYS
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCCCEEEEECCC
QIELRVLAHMSQDESFLDAFRQGQDIHTRTASEVFGVPMDQVTSEMRSRAKAVNFGIVYG
HHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEE
ISDFGLARDLKVSRKEAQGYIDHYFARCPGVRQYIDRVIKEAKETGYVNTLLNRRRYLPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LFSKNFNIRNFGERAAMNTPIQGTAADIIKLAMVKISQQLKEQNLSSQMVLQVHDELIFD
HHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
APENEIESLIMLVRQSMENALQLDVPLVVDIKLGPNWYEAKPI
CCHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SLDTIIIIDGNSLIHRAFHAIPLLSNSKGVITNAVYGFTNMVLKVIKEQEPGLVAVAFD
CCCEEEEECCCHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
KGKTTFRNQEYAAYKGTRKATPDELRPQFILARDILGAMRIPFFELDGFEADDLIGTIAC
CCCCCCCCCCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHCCCEEECCCCCHHHHHEEEEE
QAEATGLNVLILTGDRDALQLVSPKTKVMLTRKGITEIDLFDEGKVWDKYGVTPSQVVDL
EEECCCCEEEEEECCCCHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHEEEC
KGLQGDTSDNIPGVAGIGEKTASVLIKEYGSVEEILKNIDKLSPRHQKLLRGNEEIALLS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHH
KKLATIIRDVPMELDLSECSWHGPDNQKLLEIAKELEFKTLIKQLTSGQKFIKQSQDSGT
HHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
SADLPPLETYQVGYLNVTDQDALDELLIEVSRQGKVAIYLEGLRNQGIDTAYLAMEDHDI
CCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCE
YRLAIQNKDSDSDPCLTVLKAICECESVKKYFHDAKQALWMLHHHSIELKALAADTMVSA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
YLLNPTASNYDINDVALEHLGMVLPAEGETAMASRAEAIRRLAQKLFEKLAERQEDRLLT
HEECCCCCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVELPLVRILSEMEIAGVAVDKQGLQDMSVELQTVTETLTTKIHELAGENFNINSPKQLG
HHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
PILFEKLKLPVLKKTKTGYSTDAEVLEKLAEEHEIVALILEYRQMAKLKSTYTDGLAALV
HHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
DPQTGRLHSTFHQTVTATGRLSSAEPNLQNIPIRLETGRKIRKVFIPRQRGNLILTADYS
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCCCEEEEECCC
QIELRVLAHMSQDESFLDAFRQGQDIHTRTASEVFGVPMDQVTSEMRSRAKAVNFGIVYG
HHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEE
ISDFGLARDLKVSRKEAQGYIDHYFARCPGVRQYIDRVIKEAKETGYVNTLLNRRRYLPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LFSKNFNIRNFGERAAMNTPIQGTAADIIKLAMVKISQQLKEQNLSSQMVLQVHDELIFD
HHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
APENEIESLIMLVRQSMENALQLDVPLVVDIKLGPNWYEAKPI
CCHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7557480; 9016716; 9440698 [H]