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The map label for this gene is hybA [C]

Identifier: 134298337

GI number: 134298337

Start: 504447

End: 505232

Strand: Reverse

Name: hybA [C]

Synonym: Dred_0462

Alternate gene names: 134298337

Gene position: 505232-504447 (Counterclockwise)

Preceding gene: 134298338

Following gene: 134298336

Centisome position: 14.0

GC content: 43.51

Gene sequence:

>786_bases
ATGTCAAAAGGCGTACTGGTTGACCTGACTAAATGTGTAGGCTGCGGTAGTTGTACCGTTGCCTGTAAGTTATGGAACGA
CAACAAATGGGATGATAAAAACCCCACTATGGGACCAAGTGCTAAACTAACTGATAAAAACTGGACCGTGGTTCAATTTA
AAGAAGTCAAGGACAAAAAGGGTGAACCTGTCTGGCGTTTTGTTAAACAACAGTGTTTCCACTGTAAAGAACCTGCCTGT
GCATCAGCTTGCTTTGCCAAGGCATTTCAGAAGACCAAAGAAGGGCCGGTAATCTATTACCCCCACCTTTGTGTTGGTTG
CCGTTACTGCATGGTGGCATGTCCTTTCAATGTTCCTAAATATCAATGGGAAAAAGCCTTCCCGCTGGTTACCAAATGCA
TGATGTGCAATACCAAGGTTGAAAAGGGCGAAGCCCCTGCCTGTGTATCGGTTTGTCCTGCCGGTGTTTTCAAGTTTGGG
GATCGGGAAGAACTTTTAAAAGAAGCAAAATCCATCATTGCCAAGGATTCAAAGTATGTTAAGCATATCTTCGGTGAAAA
TGAAGTGGGTGGTACCGAGTGGCTTTACATTTCCGATGTACCCTTTGATCAGCTAGGGTTTAAAACCAACGTTACCAAAC
GTTCCCTACCCAGCTATACCGAAGCTTACATGCATGCAACTCCCTTTGTTGGTATTACCTGGGGCGTCATTCTAACCGGT
TTATACCATTACACAAAGCGTCGCAACCAAATTTCAAAAGACAATAACAAAAATATTAAGGCCTAA

Upstream 100 bases:

>100_bases
GCTCACGAACTGCTGCACACCCACTATACCGACCGCAGCAAGCAACTGACTGTGAAAAAGAAAAATGCGTAATTCTGCAT
ATTCCGGGAGGTGAAGAACC

Downstream 100 bases:

>100_bases
TACAATTCAACTATTTCTGAGAACTGGGGGGATATACATGGCGTCTCACGCCCAAGCCCAAAAGTGGAGTTTTAAAATGA
CTCCCACACGCGTATTATTA

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; NADH

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSKGVLVDLTKCVGCGSCTVACKLWNDNKWDDKNPTMGPSAKLTDKNWTVVQFKEVKDKKGEPVWRFVKQQCFHCKEPAC
ASACFAKAFQKTKEGPVIYYPHLCVGCRYCMVACPFNVPKYQWEKAFPLVTKCMMCNTKVEKGEAPACVSVCPAGVFKFG
DREELLKEAKSIIAKDSKYVKHIFGENEVGGTEWLYISDVPFDQLGFKTNVTKRSLPSYTEAYMHATPFVGITWGVILTG
LYHYTKRRNQISKDNNKNIKA

Sequences:

>Translated_261_residues
MSKGVLVDLTKCVGCGSCTVACKLWNDNKWDDKNPTMGPSAKLTDKNWTVVQFKEVKDKKGEPVWRFVKQQCFHCKEPAC
ASACFAKAFQKTKEGPVIYYPHLCVGCRYCMVACPFNVPKYQWEKAFPLVTKCMMCNTKVEKGEAPACVSVCPAGVFKFG
DREELLKEAKSIIAKDSKYVKHIFGENEVGGTEWLYISDVPFDQLGFKTNVTKRSLPSYTEAYMHATPFVGITWGVILTG
LYHYTKRRNQISKDNNKNIKA
>Mature_260_residues
SKGVLVDLTKCVGCGSCTVACKLWNDNKWDDKNPTMGPSAKLTDKNWTVVQFKEVKDKKGEPVWRFVKQQCFHCKEPACA
SACFAKAFQKTKEGPVIYYPHLCVGCRYCMVACPFNVPKYQWEKAFPLVTKCMMCNTKVEKGEAPACVSVCPAGVFKFGD
REELLKEAKSIIAKDSKYVKHIFGENEVGGTEWLYISDVPFDQLGFKTNVTKRSLPSYTEAYMHATPFVGITWGVILTGL
YHYTKRRNQISKDNNKNIKA

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=285, Percent_Identity=34.7368421052632, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787749, Length=199, Percent_Identity=36.6834170854271, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI1790326, Length=199, Percent_Identity=35.678391959799, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787872, Length=177, Percent_Identity=31.0734463276836, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787122, Length=177, Percent_Identity=29.9435028248588, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367345, Length=178, Percent_Identity=30.3370786516854, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI226510944, Length=162, Percent_Identity=30.2469135802469, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367245, Length=168, Percent_Identity=27.3809523809524, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082180, Length=166, Percent_Identity=25.9036144578313, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 29478; Mature: 29347

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGVLVDLTKCVGCGSCTVACKLWNDNKWDDKNPTMGPSAKLTDKNWTVVQFKEVKDKK
CCCCEEEEHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCC
GEPVWRFVKQQCFHCKEPACASACFAKAFQKTKEGPVIYYPHLCVGCRYCMVACPFNVPK
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCC
YQWEKAFPLVTKCMMCNTKVEKGEAPACVSVCPAGVFKFGDREELLKEAKSIIAKDSKYV
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCHHHH
KHIFGENEVGGTEWLYISDVPFDQLGFKTNVTKRSLPSYTEAYMHATPFVGITWGVILTG
HHHCCCCCCCCCEEEEEECCCHHHHCCCCCCCHHCCCHHHHHHHHCCCCHHHHHHHHHHH
LYHYTKRRNQISKDNNKNIKA
HHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKGVLVDLTKCVGCGSCTVACKLWNDNKWDDKNPTMGPSAKLTDKNWTVVQFKEVKDKK
CCCEEEEHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCC
GEPVWRFVKQQCFHCKEPACASACFAKAFQKTKEGPVIYYPHLCVGCRYCMVACPFNVPK
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCC
YQWEKAFPLVTKCMMCNTKVEKGEAPACVSVCPAGVFKFGDREELLKEAKSIIAKDSKYV
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCHHHH
KHIFGENEVGGTEWLYISDVPFDQLGFKTNVTKRSLPSYTEAYMHATPFVGITWGVILTG
HHHCCCCCCCCCEEEEEECCCHHHHCCCCCCCHHCCCHHHHHHHHCCCCHHHHHHHHHHH
LYHYTKRRNQISKDNNKNIKA
HHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]