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The map label for this gene is nirB [C]

Identifier: 134298048

GI number: 134298048

Start: 178120

End: 178776

Strand: Reverse

Name: nirB [C]

Synonym: Dred_0170

Alternate gene names: 134298048

Gene position: 178776-178120 (Counterclockwise)

Preceding gene: 134298062

Following gene: 134298036

Centisome position: 4.95

GC content: 44.29

Gene sequence:

>657_bases
ATGTCAGAATCCCCCAAAAATGCTATCTTACAACGTGATAATACCTATGCAATTATCCCTAAAACTGGTTTCGCCGGGTT
ATTAAACGGTGACTCCTTACGTAAGTTGGCCGATGTTGTGGACAAGTATCAAATTCCGGTGATAAAGATGACTGCTTCCC
AACGAATCGCATTGGTGGGCATTAAAGAAGAAGATGTAAACAAAGTATGGGCAGACCTCGGAATGGAAGCTGCACCTGCC
ACAGGTCTCTGTGTAAGAAGTGTGCAATCCTGTCCAGGAAATTCCCTGTGCAAATATGGTAAACAGGATTCCTTAGGACT
GGCCCAAAGGCTAGATGAACTTTTCTCTGGCATAGCATTGCCTAATAAATTTAAAATGGGTATTTCTGGATGCCCATTAA
ATTGTTGCGAAGCTTGGACGAGGGATTTTGGGGCCTTTGGAAAACCAAATGGCTTCACCGTTGTGATTGGCGGCAACGCC
GGTGCCGCTCCCCACATTGGTAAAGTGCTGGCAGAGGGCCTGAGTATAGAAGAGGTTGTATCCCTGGCTGGTCGGTTAAT
TAGTTATTATAAAGAGAATGCTAAGAACGGCGAGAGAATGTTTAAATTTGTAGAGCGACTGGGCATTGAAGAAATACAAA
AGGTCACCTTAGCTTAA

Upstream 100 bases:

>100_bases
TAATTTTTATTTTTCTGTATTATTTTGTTTTCCCTTGACATTAAAGTTATCTTTTGTATACTATTTTAGTATACTTTTTG
TTGTGAAAGGAGCTATGCTC

Downstream 100 bases:

>100_bases
TGCAAAAGCTGCCGAGGTTAACTCGGCAGCTTTTGGTTTGGCTTGGTTGAAGAATTAGGCAGTCGTTGATGAATGGCTCT
TGCCAAAACAGCCACAATTA

Product: nitrite and sulfite reductase 4Fe-4S subunit

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSESPKNAILQRDNTYAIIPKTGFAGLLNGDSLRKLADVVDKYQIPVIKMTASQRIALVGIKEEDVNKVWADLGMEAAPA
TGLCVRSVQSCPGNSLCKYGKQDSLGLAQRLDELFSGIALPNKFKMGISGCPLNCCEAWTRDFGAFGKPNGFTVVIGGNA
GAAPHIGKVLAEGLSIEEVVSLAGRLISYYKENAKNGERMFKFVERLGIEEIQKVTLA

Sequences:

>Translated_218_residues
MSESPKNAILQRDNTYAIIPKTGFAGLLNGDSLRKLADVVDKYQIPVIKMTASQRIALVGIKEEDVNKVWADLGMEAAPA
TGLCVRSVQSCPGNSLCKYGKQDSLGLAQRLDELFSGIALPNKFKMGISGCPLNCCEAWTRDFGAFGKPNGFTVVIGGNA
GAAPHIGKVLAEGLSIEEVVSLAGRLISYYKENAKNGERMFKFVERLGIEEIQKVTLA
>Mature_217_residues
SESPKNAILQRDNTYAIIPKTGFAGLLNGDSLRKLADVVDKYQIPVIKMTASQRIALVGIKEEDVNKVWADLGMEAAPAT
GLCVRSVQSCPGNSLCKYGKQDSLGLAQRLDELFSGIALPNKFKMGISGCPLNCCEAWTRDFGAFGKPNGFTVVIGGNAG
AAPHIGKVLAEGLSIEEVVSLAGRLISYYKENAKNGERMFKFVERLGIEEIQKVTLA

Specific function: This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789765, Length=215, Percent_Identity=29.7674418604651, Blast_Score=117, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR017220 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 23455; Mature: 23324

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESPKNAILQRDNTYAIIPKTGFAGLLNGDSLRKLADVVDKYQIPVIKMTASQRIALVG
CCCCCCCHHEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEE
IKEEDVNKVWADLGMEAAPATGLCVRSVQSCPGNSLCKYGKQDSLGLAQRLDELFSGIAL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCC
PNKFKMGISGCPLNCCEAWTRDFGAFGKPNGFTVVIGGNAGAAPHIGKVLAEGLSIEEVV
CCHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHH
SLAGRLISYYKENAKNGERMFKFVERLGIEEIQKVTLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
SESPKNAILQRDNTYAIIPKTGFAGLLNGDSLRKLADVVDKYQIPVIKMTASQRIALVG
CCCCCCHHEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEE
IKEEDVNKVWADLGMEAAPATGLCVRSVQSCPGNSLCKYGKQDSLGLAQRLDELFSGIAL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCC
PNKFKMGISGCPLNCCEAWTRDFGAFGKPNGFTVVIGGNAGAAPHIGKVLAEGLSIEEVV
CCHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHH
SLAGRLISYYKENAKNGERMFKFVERLGIEEIQKVTLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1911781 [H]