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The map label for this gene is cca [H]

Identifier: 134298036

GI number: 134298036

Start: 166455

End: 167873

Strand: Reverse

Name: cca [H]

Synonym: Dred_0158

Alternate gene names: 134298036

Gene position: 167873-166455 (Counterclockwise)

Preceding gene: 134298048

Following gene: 134298035

Centisome position: 4.65

GC content: 43.13

Gene sequence:

>1419_bases
TTGAGTACCATCAGTATGCTTTCTCAACAGCTAAGCATTCAAACCTATCTGGTGGGTGGTGCTGTGAGAGATTTATTCTT
AAACAAGATACCCGTTGACCTAGATTTTTGTGTGACAGCTAAGGTTTTTGCATTGGCCCAAACTTTGACCCAACAATATC
AGGGAAGCCTGGTTACACTGGATGACCAACGTGAAATGCTACGGGTTGTAACAAATTCGTGGCATCTTGATTTTAGCCCT
CTGCGGGATGAAATCCTGGAAAAGGACCTTTTTGCCAGAGACTTTACACTCAATGCCATGGCTCTACCTGTATCGATGGG
TATTAACAAGTATAATTGGCAAAATCAAATACAAGACCCAACAGGGGGTAAAGGGGATTTAAAAAAAGGTTTACTGCGGG
CTGCATCAAGTTCCTCTATCCAACAGGACCCTCTGCGGTCCCTGCGTGGCATACGCTTTGCAGCCACATACAATCTAGCC
ATTATTCCAGAAACCATGGTATTGCTTAGACAAGGTACCCGCCGTATTGAACAGATCGCCGGCGAACGGCTTTGGCATGA
GCTAGCTATCCTTTTTAAATTACCAATCACATCATTCTGGATTAATTATATGGATCAAGAATTACATTTTTGGCAGTATT
TATTACCTGGCCAATTACGCATGGCTCAAACTAAACAAAATCACTATCATGTTGAAAACGTTTGGCGTCACTGTCTGCGT
ACCTATGAATGTCTTGAGGTGATTTTACAAGAATTATCCACCATACCGTCAGAAGGAGAACCGTTGCTTATTCATTTTAA
TCAACACCTATCCGGTGGCAGAAGTCGTTGCCAAGTACTTAAGCTGGCAGCTCTTATTCATGATGTGGGAAAGCCCGATA
CCGCTGTAATCAGAGAAGATGGCCGCATTTCTTTTCATGGTCATGCCGAAGCAGGCGTTCCCTATGCTGAAGCCCTGGCA
ACACGGCTCAAGCTTTCCCGGCTTGAGAGACATTACCTTGTAAATCTGGTGCTTCTCCATATGCTGCCCCTAAACCTTTA
TAACTCTGGGGACCGTTCTGATCTGTCCGTATACCGATTATTTCGTTCTCTGGAAAATTACATTCTAGATGTTCTTATCT
TATCACTGGCTGACGTAACTGCCACTTTAACCGCTGGAGAAAGAATCAGTGAATTAGAAGCCTATCGTACATTTATTTTG
AATCTCCTCGGTAAATTTATAGTAGAAAAGGACAGGTTCCATCCAGTGCCTTATCTATCAGGTGCGGCTTTGCTTGAACT
GGGTATCCCTGAAGGCCCAGAGGTGGGGCTTATACTAGAAAAGTTATGTGAAGAACAAGTCACCGGTTCTATCACCGATA
CAAAAACTGCACTCCTATGGGTAAAAACAAAGCTAGCCGGAAATAATAAAGATAAATAA

Upstream 100 bases:

>100_bases
GTAGATAAATTACCCATTGTATAGGATTGTTAGGGGTTGCAAATAATTTAATGATTCCCTGCGGTCTTATTCTGGACGGT
GATAATTCGTGTGGAGCTTA

Downstream 100 bases:

>100_bases
GTTTTTCAAGGAGTGTATACCATGCCAACGATCAGAGTTGAGGGCTTTAATGAGGCGCCGGATTATATGGTCGAAAAAGT
GCTGATGGATAATACCCCTA

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSTISMLSQQLSIQTYLVGGAVRDLFLNKIPVDLDFCVTAKVFALAQTLTQQYQGSLVTLDDQREMLRVVTNSWHLDFSP
LRDEILEKDLFARDFTLNAMALPVSMGINKYNWQNQIQDPTGGKGDLKKGLLRAASSSSIQQDPLRSLRGIRFAATYNLA
IIPETMVLLRQGTRRIEQIAGERLWHELAILFKLPITSFWINYMDQELHFWQYLLPGQLRMAQTKQNHYHVENVWRHCLR
TYECLEVILQELSTIPSEGEPLLIHFNQHLSGGRSRCQVLKLAALIHDVGKPDTAVIREDGRISFHGHAEAGVPYAEALA
TRLKLSRLERHYLVNLVLLHMLPLNLYNSGDRSDLSVYRLFRSLENYILDVLILSLADVTATLTAGERISELEAYRTFIL
NLLGKFIVEKDRFHPVPYLSGAALLELGIPEGPEVGLILEKLCEEQVTGSITDTKTALLWVKTKLAGNNKDK

Sequences:

>Translated_472_residues
MSTISMLSQQLSIQTYLVGGAVRDLFLNKIPVDLDFCVTAKVFALAQTLTQQYQGSLVTLDDQREMLRVVTNSWHLDFSP
LRDEILEKDLFARDFTLNAMALPVSMGINKYNWQNQIQDPTGGKGDLKKGLLRAASSSSIQQDPLRSLRGIRFAATYNLA
IIPETMVLLRQGTRRIEQIAGERLWHELAILFKLPITSFWINYMDQELHFWQYLLPGQLRMAQTKQNHYHVENVWRHCLR
TYECLEVILQELSTIPSEGEPLLIHFNQHLSGGRSRCQVLKLAALIHDVGKPDTAVIREDGRISFHGHAEAGVPYAEALA
TRLKLSRLERHYLVNLVLLHMLPLNLYNSGDRSDLSVYRLFRSLENYILDVLILSLADVTATLTAGERISELEAYRTFIL
NLLGKFIVEKDRFHPVPYLSGAALLELGIPEGPEVGLILEKLCEEQVTGSITDTKTALLWVKTKLAGNNKDK
>Mature_471_residues
STISMLSQQLSIQTYLVGGAVRDLFLNKIPVDLDFCVTAKVFALAQTLTQQYQGSLVTLDDQREMLRVVTNSWHLDFSPL
RDEILEKDLFARDFTLNAMALPVSMGINKYNWQNQIQDPTGGKGDLKKGLLRAASSSSIQQDPLRSLRGIRFAATYNLAI
IPETMVLLRQGTRRIEQIAGERLWHELAILFKLPITSFWINYMDQELHFWQYLLPGQLRMAQTKQNHYHVENVWRHCLRT
YECLEVILQELSTIPSEGEPLLIHFNQHLSGGRSRCQVLKLAALIHDVGKPDTAVIREDGRISFHGHAEAGVPYAEALAT
RLKLSRLERHYLVNLVLLHMLPLNLYNSGDRSDLSVYRLFRSLENYILDVLILSLADVTATLTAGERISELEAYRTFILN
LLGKFIVEKDRFHPVPYLSGAALLELGIPEGPEVGLILEKLCEEQVTGSITDTKTALLWVKTKLAGNNKDK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=228, Percent_Identity=28.0701754385965, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 53533; Mature: 53402

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTISMLSQQLSIQTYLVGGAVRDLFLNKIPVDLDFCVTAKVFALAQTLTQQYQGSLVTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
DDQREMLRVVTNSWHLDFSPLRDEILEKDLFARDFTLNAMALPVSMGINKYNWQNQIQDP
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCC
TGGKGDLKKGLLRAASSSSIQQDPLRSLRGIRFAATYNLAIIPETMVLLRQGTRRIEQIA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHH
GERLWHELAILFKLPITSFWINYMDQELHFWQYLLPGQLRMAQTKQNHYHVENVWRHCLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHHHHH
TYECLEVILQELSTIPSEGEPLLIHFNQHLSGGRSRCQVLKLAALIHDVGKPDTAVIRED
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECC
GRISFHGHAEAGVPYAEALATRLKLSRLERHYLVNLVLLHMLPLNLYNSGDRSDLSVYRL
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
FRSLENYILDVLILSLADVTATLTAGERISELEAYRTFILNLLGKFIVEKDRFHPVPYLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GAALLELGIPEGPEVGLILEKLCEEQVTGSITDTKTALLWVKTKLAGNNKDK
CCHHEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
STISMLSQQLSIQTYLVGGAVRDLFLNKIPVDLDFCVTAKVFALAQTLTQQYQGSLVTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
DDQREMLRVVTNSWHLDFSPLRDEILEKDLFARDFTLNAMALPVSMGINKYNWQNQIQDP
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCC
TGGKGDLKKGLLRAASSSSIQQDPLRSLRGIRFAATYNLAIIPETMVLLRQGTRRIEQIA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHH
GERLWHELAILFKLPITSFWINYMDQELHFWQYLLPGQLRMAQTKQNHYHVENVWRHCLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHHHHH
TYECLEVILQELSTIPSEGEPLLIHFNQHLSGGRSRCQVLKLAALIHDVGKPDTAVIRED
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECC
GRISFHGHAEAGVPYAEALATRLKLSRLERHYLVNLVLLHMLPLNLYNSGDRSDLSVYRL
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
FRSLENYILDVLILSLADVTATLTAGERISELEAYRTFILNLLGKFIVEKDRFHPVPYLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GAALLELGIPEGPEVGLILEKLCEEQVTGSITDTKTALLWVKTKLAGNNKDK
CCHHEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA