Click here to switch to the map view.

The map label for this gene is ksgA

Identifier: 13358166

GI number: 13358166

Start: 749031

End: 749804

Strand: Reverse

Name: ksgA

Synonym: UU601

Alternate gene names: 13358166

Gene position: 749804-749031 (Counterclockwise)

Preceding gene: 13358167

Following gene: 13358165

Centisome position: 99.75

GC content: 22.74

Gene sequence:

>774_bases
ATGGGGCAAAACTTCTTATTGTCTAATAACATAAAAAATAAAATTGTAGATGTGGCGAATATTAATAAAGATGATCTTAT
ATTAGAAATTGGTCCAGGGTGAGGAGCAATTACTGAAATTCTTGTACAAAAAACAAATATTTTAATTGCTATTGAATTAG
ATAAAAGACTATATGCACACTTAAAAACATACATAAAAACAAGTAATTTTCATATAATAAATAATGATGTATTATGTGTA
GATTTAGATAATTTGATTTTAGATTACAATAATACTCAAAAAATTCAAAAAATTAAAGTTGTTGCAAATCTTCCATATGC
AATATCTTCAAAGATAGTTTTAAAAATTATTCAATCAAAATTAATTAATGATGCTTATATTATGGTTCAAAAAGAAATGG
CTGAACGAATTGGTGCTAAAGTAAATACACGTGGTTATAATGCTTTTACTGTTTTAGTACAATTATTCTGTAAAACAAAA
ATTCTATTCGAAGTTAATGCTAAAGAATTTCATCCCCAACCTAAAGTTCAAAGTGCTGTCATTCATCTTGAAAATCTTCA
TAATAGTGTGAATTTTAATATTGAAGAATTAGGTAAATTTTTACGGATTTGTTTTTTAAATAAACGAAAAAAGTTAAAAA
ATAACTTATCTAATATTTATGATATTAAAATAATTAATCAAATGTTTATTGATTATAATTTAGATATGAATTTACGAGCT
GAAAACATTGAACCAAAAATGTTTCTAAAACTCTTTAATTATTTGAATAGATAA

Upstream 100 bases:

>100_bases
AGAATTGAATCAAAACTTGCTAAATTAGGAATTAGCTAACTTGAATAAATCATTCATTAAAAATAAACTCAAGCAAGAAA
GTTTTGTTCCATCAAAAAAA

Downstream 100 bases:

>100_bases
GATAATTAATAAAATTAAGTTGTAGGTCAAATTATGAAAGTAAAGTCATTCGCAAAAATTGATTTAGGATATAGTGTTTA
TAAAAAACAAAAGAATTTTA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MGQNFLLSNNIKNKIVDVANINKDDLILEIGPGWGAITEILVQKTNILIAIELDKRLYAHLKTYIKTSNFHIINNDVLCV
DLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTK
ILFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMNLRA
ENIEPKMFLKLFNYLNR

Sequences:

>Translated_257_residues
MGQNFLLSNNIKNKIVDVANINKDDLILEIGPG*GAITEILVQKTNILIAIELDKRLYAHLKTYIKTSNFHIINNDVLCV
DLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTK
ILFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMNLRA
ENIEPKMFLKLFNYLNR
>Mature_256_residues
GQNFLLSNNIKNKIVDVANINKDDLILEIGPG*GAITEILVQKTNILIAIELDKRLYAHLKTYIKTSNFHIINNDVLCVD
LDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKI
LFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMNLRAE
NIEPKMFLKLFNYLNR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=217, Percent_Identity=33.6405529953917, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI156415992, Length=201, Percent_Identity=25.8706467661692, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1786236, Length=260, Percent_Identity=31.5384615384615, Blast_Score=128, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25146882, Length=228, Percent_Identity=32.0175438596491, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25141369, Length=253, Percent_Identity=23.3201581027668, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324989, Length=240, Percent_Identity=27.5, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21358017, Length=248, Percent_Identity=29.4354838709677, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21357273, Length=199, Percent_Identity=29.6482412060301, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 29577; Mature: 29446

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQNFLLSNNIKNKIVDVANINKDDLILEIGPGGAITEILVQKTNILIAIELDKRLYAHL
CCCCEEEECCCHHHEEEEECCCCCCEEEEECCCCHHHHHHHHHCCEEEEEEECHHHHHHH
KTYIKTSNFHIINNDVLCVDLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKL
HHHHCCCCEEEECCCEEEEEHHHEEEECCCCHHHHEEEEEEECCHHHHHHHHHHHHHHHH
INDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAVI
CCHHHEEEEHHHHHHHCCEECCCCCHHHHHHHHHHHCCEEEEEECHHHCCCCCCHHHHHH
HLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMNLRAE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHEEEEEEHEEECCCCEEEEC
NIEPKMFLKLFNYLNR
CCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GQNFLLSNNIKNKIVDVANINKDDLILEIGPGGAITEILVQKTNILIAIELDKRLYAHL
CCCEEEECCCHHHEEEEECCCCCCEEEEECCCCHHHHHHHHHCCEEEEEEECHHHHHHH
KTYIKTSNFHIINNDVLCVDLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKL
HHHHCCCCEEEECCCEEEEEHHHEEEECCCCHHHHEEEEEEECCHHHHHHHHHHHHHHHH
INDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAVI
CCHHHEEEEHHHHHHHCCEECCCCCHHHHHHHHHHHCCEEEEEECHHHCCCCCCHHHHHH
HLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMNLRAE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHEEEEEEHEEECCCCEEEEC
NIEPKMFLKLFNYLNR
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA