Click here to switch to the map view.

The map label for this gene is infB

Identifier: 13357880

GI number: 13357880

Start: 366428

End: 368272

Strand: Direct

Name: infB

Synonym: UU320

Alternate gene names: 13357880

Gene position: 366428-368272 (Clockwise)

Preceding gene: 13357879

Following gene: 13357881

Centisome position: 48.75

GC content: 29.65

Gene sequence:

>1845_bases
ATGGCGAAAAAAAATATCAAACAAAAGAAAGATAATCGGATAGCAATCGATGTAAAAAAACACATTAAAAAAGTTGATGT
TGGGGTTTTTGATGGTACATTTGTTTTCACATCACCTCTTAGTATTTCAGAATTAGCACCAAAACTAAATAAAAGTCCAA
ACGAAATTATTATGCGTTATTTCAAAAAAGGTGTTGTTTATAACTTAAACACTATTTTAGATGAAGAACAAATTGGTGAG
TTATGTTTAGAATATGATTTAGATTTTAAAATTGAAAAGAATGTTAACACTGAAAATCTTTTAGAAAATATTTATTTTGA
TGATCTAGAAATTGATTTGGTTGCTCGTGCTCCAATTGTAACAATTATGGGTCATGTTGATCATGGTAAAACAACACTTT
TAGATACTATTCGTAAATCATCAATAACAGCATCAGAAGCTGGAGGAATTACTCAACATATTGGAGCTTATCAAATTATA
AAAGACAATAGAGCAATTACTTTTATTGATACACCCGGACATGAAGCCTTTACTGAAATGCGTGCTCGCGGAGCAAATTT
AACAGATATTGTGATTTTAGTTGTTGCTGCTGATGATGGTATTAAAATGCAAACTGAAGAAGCAATTGATCATGCTAAAG
CTGCTAATGTTCCTATTATTGTTTTTGTTAATAAAATGGATAAATACGAAGCAAATCCTGAAAAAGTTTTAAATCAATTA
AGTGCTAAAGAAATTGTTGCTGAAGAGCTTGGTGGTGATGTTGTTTTTGTTAAAGGCTCAGCATTGAAAAATGAAGGAAT
TTCTGAATTATTAGATTCAATTTTATTGATTGCTGAATTAAATAATTACAAAGCTAATCCTAATCGTTTAGCTTATGGAA
CTACAATTGAAGCAAATTTGGATAAAGGTCATGGTCCTCTTGCTACATTGTTAGTTCAAAATGGCACATTACGAAAAGGT
GATTATTTAGTTGTTGGTTCAACATATGGAAAAATTCGTAATATGTTTGATGAATATGACAACGAAATTGAAATAGCATT
ACCTTCAAAACCTGTTAAAGTTTCTGGATTTGAAGAAGTACCAACAGCCGGTGATAAATTTTTAGCTTTAGCTGATGAAA
AACAAGCACGTGCAATAGCAAATGATGTTAAACAAAAGAAAATGCGTTTAGAACGTGCAATGTTACAAAGTTCGGATATT
CGAACAAAAATTGCTAATGGTGAATTAAAAAATATTAATTTAATTATTAAAGCTGATGTTCAAGGTTCACTTGAAGCTTT
AAAAGGAATTTTTAGTAGCATTAACATTGAAGGTGTGACTACGACACTAATACGGAGTGCAATTGGAACAATTAGTGAAA
GTGATGTGCGTTTAGCACAGACTTCTGATGCAATAATTATTGGTTTTAATGTCCGTGCTAGTCGAATTATTAAAGATTTA
GCTGATTCAGTCGGTGTACAAATTATGAATTATGACATTATTTATAAATTTAAAGAAGATTTAGAATTATGAATGAAAGG
TACATTAGACCCAATTATTATTGAAGAAGTGATTGGCGAAGCCAAAGTTCTTAAATTATTTAAACACAGTCAAGTTGGAA
CAATTTGTGGTTGTCGAGTAATTAATGGTAAAATTAAAAGAAATGCTTTGGTTCGTGTTTTACGTGATGGTATTGTTATA
TATAATTCAAAAATTGCGACATTACAACACAATAAAGATAGTGTTAATGAAGTTATTGCTGATAAAGAATGCGGATTAAC
AATTGCAAACTTTAATGATATTAAAGAGAACGATATTATTGAAGTTTATATTAAAGTTGAAAAGAAACATGATGAGGTTA
AATAA

Upstream 100 bases:

>100_bases
TTTATGATTAAAAACGAAGAACAAGTTTATCGGATTTTAGAGCAATTAATTCAATCATTAATTTAAAATATATAGAGTAA
TATAAGTAAGAGGTAAAAAT

Downstream 100 bases:

>100_bases
ATAAAATATGGCAAACGAAGTTCGAGTTGCAAGGTTAGAATCTTTAATTAAAGATGTTATTAATAATGCGTTAGCGAATG
AAATTAATGATAAAATTGCA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MAKKNIKQKKDNRIAIDVKKHIKKVDVGVFDGTFVFTSPLSISELAPKLNKSPNEIIMRYFKKGVVYNLNTILDEEQIGE
LCLEYDLDFKIEKNVNTENLLENIYFDDLEIDLVARAPIVTIMGHVDHGKTTLLDTIRKSSITASEAGGITQHIGAYQII
KDNRAITFIDTPGHEAFTEMRARGANLTDIVILVVAADDGIKMQTEEAIDHAKAANVPIIVFVNKMDKYEANPEKVLNQL
SAKEIVAEELGGDVVFVKGSALKNEGISELLDSILLIAELNNYKANPNRLAYGTTIEANLDKGHGPLATLLVQNGTLRKG
DYLVVGSTYGKIRNMFDEYDNEIEIALPSKPVKVSGFEEVPTAGDKFLALADEKQARAIANDVKQKKMRLERAMLQSSDI
RTKIANGELKNINLIIKADVQGSLEALKGIFSSINIEGVTTTLIRSAIGTISESDVRLAQTSDAIIIGFNVRASRIIKDL
ADSVGVQIMNYDIIYKFKEDLELWMKGTLDPIIIEEVIGEAKVLKLFKHSQVGTICGCRVINGKIKRNALVRVLRDGIVI
YNSKIATLQHNKDSVNEVIADKECGLTIANFNDIKENDIIEVYIKVEKKHDEVK

Sequences:

>Translated_614_residues
MAKKNIKQKKDNRIAIDVKKHIKKVDVGVFDGTFVFTSPLSISELAPKLNKSPNEIIMRYFKKGVVYNLNTILDEEQIGE
LCLEYDLDFKIEKNVNTENLLENIYFDDLEIDLVARAPIVTIMGHVDHGKTTLLDTIRKSSITASEAGGITQHIGAYQII
KDNRAITFIDTPGHEAFTEMRARGANLTDIVILVVAADDGIKMQTEEAIDHAKAANVPIIVFVNKMDKYEANPEKVLNQL
SAKEIVAEELGGDVVFVKGSALKNEGISELLDSILLIAELNNYKANPNRLAYGTTIEANLDKGHGPLATLLVQNGTLRKG
DYLVVGSTYGKIRNMFDEYDNEIEIALPSKPVKVSGFEEVPTAGDKFLALADEKQARAIANDVKQKKMRLERAMLQSSDI
RTKIANGELKNINLIIKADVQGSLEALKGIFSSINIEGVTTTLIRSAIGTISESDVRLAQTSDAIIIGFNVRASRIIKDL
ADSVGVQIMNYDIIYKFKEDLEL*MKGTLDPIIIEEVIGEAKVLKLFKHSQVGTICGCRVINGKIKRNALVRVLRDGIVI
YNSKIATLQHNKDSVNEVIADKECGLTIANFNDIKENDIIEVYIKVEKKHDEVK
>Mature_613_residues
AKKNIKQKKDNRIAIDVKKHIKKVDVGVFDGTFVFTSPLSISELAPKLNKSPNEIIMRYFKKGVVYNLNTILDEEQIGEL
CLEYDLDFKIEKNVNTENLLENIYFDDLEIDLVARAPIVTIMGHVDHGKTTLLDTIRKSSITASEAGGITQHIGAYQIIK
DNRAITFIDTPGHEAFTEMRARGANLTDIVILVVAADDGIKMQTEEAIDHAKAANVPIIVFVNKMDKYEANPEKVLNQLS
AKEIVAEELGGDVVFVKGSALKNEGISELLDSILLIAELNNYKANPNRLAYGTTIEANLDKGHGPLATLLVQNGTLRKGD
YLVVGSTYGKIRNMFDEYDNEIEIALPSKPVKVSGFEEVPTAGDKFLALADEKQARAIANDVKQKKMRLERAMLQSSDIR
TKIANGELKNINLIIKADVQGSLEALKGIFSSINIEGVTTTLIRSAIGTISESDVRLAQTSDAIIIGFNVRASRIIKDLA
DSVGVQIMNYDIIYKFKEDLEL*MKGTLDPIIIEEVIGEAKVLKLFKHSQVGTICGCRVINGKIKRNALVRVLRDGIVIY
NSKIATLQHNKDSVNEVIADKECGLTIANFNDIKENDIIEVYIKVEKKHDEVK

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=521, Percent_Identity=40.3071017274472, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI53729337, Length=521, Percent_Identity=40.3071017274472, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI84043963, Length=503, Percent_Identity=27.6341948310139, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI157426893, Length=242, Percent_Identity=27.6859504132231, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI18390331, Length=146, Percent_Identity=31.5068493150685, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1789559, Length=574, Percent_Identity=44.4250871080139, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI2367247, Length=213, Percent_Identity=31.4553990610329, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790412, Length=229, Percent_Identity=32.3144104803493, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1789737, Length=229, Percent_Identity=32.3144104803493, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788922, Length=231, Percent_Identity=29.004329004329, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI48994988, Length=137, Percent_Identity=32.1167883211679, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=544, Percent_Identity=38.9705882352941, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI212656558, Length=559, Percent_Identity=27.5491949910555, Blast_Score=139, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324550, Length=643, Percent_Identity=37.3250388802488, Blast_Score=344, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6319282, Length=459, Percent_Identity=27.6688453159041, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324761, Length=242, Percent_Identity=29.7520661157025, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28572034, Length=532, Percent_Identity=38.5338345864662, Blast_Score=342, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24656849, Length=458, Percent_Identity=29.4759825327511, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI160714833, Length=73, Percent_Identity=63.013698630137, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI19922690, Length=172, Percent_Identity=33.7209302325581, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24582462, Length=146, Percent_Identity=32.8767123287671, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 67845; Mature: 67714

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNIKQKKDNRIAIDVKKHIKKVDVGVFDGTFVFTSPLSISELAPKLNKSPNEIIMRY
CCCCCCCCCCCCEEEEEHHHHHHHHCCEEECCEEEEECCCCHHHHHHHHCCCHHHHHHHH
FKKGVVYNLNTILDEEQIGELCLEYDLDFKIEKNVNTENLLENIYFDDLEIDLVARAPIV
HHCCCEEEHHHHCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCEEEEEECCCEE
TIMGHVDHGKTTLLDTIRKSSITASEAGGITQHIGAYQIIKDNRAITFIDTPGHEAFTEM
EEEECCCCCHHHHHHHHHHHCCCCHHCCCCHHHCCCEEEEECCCEEEEEECCCHHHHHHH
RARGANLTDIVILVVAADDGIKMQTEEAIDHAKAANVPIIVFVNKMDKYEANPEKVLNQL
HHCCCCCEEEEEEEEECCCCCEECHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHH
SAKEIVAEELGGDVVFVKGSALKNEGISELLDSILLIAELNNYKANPNRLAYGTTIEANL
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECC
DKGHGPLATLLVQNGTLRKGDYLVVGSTYGKIRNMFDEYDNEIEIALPSKPVKVSGFEEV
CCCCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCCEEECCCCCC
PTAGDKFLALADEKQARAIANDVKQKKMRLERAMLQSSDIRTKIANGELKNINLIIKADV
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEECC
QGSLEALKGIFSSINIEGVTTTLIRSAIGTISESDVRLAQTSDAIIIGFNVRASRIIKDL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCHHHHHHHHH
ADSVGVQIMNYDIIYKFKEDLELMKGTLDPIIIEEVIGEAKVLKLFKHSQVGTICGCRVI
HHHHCEEEEEEEEEEEEHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEE
NGKIKRNALVRVLRDGIVIYNSKIATLQHNKDSVNEVIADKECGLTIANFNDIKENDIIE
CCHHHHHHHHHHHHCCEEEEECEEEEEECCHHHHHHHHCCCCCCEEEECCCCCCCCCEEE
VYIKVEKKHDEVK
EEEEEECCCCCCC
>Mature Secondary Structure 
AKKNIKQKKDNRIAIDVKKHIKKVDVGVFDGTFVFTSPLSISELAPKLNKSPNEIIMRY
CCCCCCCCCCCEEEEEHHHHHHHHCCEEECCEEEEECCCCHHHHHHHHCCCHHHHHHHH
FKKGVVYNLNTILDEEQIGELCLEYDLDFKIEKNVNTENLLENIYFDDLEIDLVARAPIV
HHCCCEEEHHHHCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCEEEEEECCCEE
TIMGHVDHGKTTLLDTIRKSSITASEAGGITQHIGAYQIIKDNRAITFIDTPGHEAFTEM
EEEECCCCCHHHHHHHHHHHCCCCHHCCCCHHHCCCEEEEECCCEEEEEECCCHHHHHHH
RARGANLTDIVILVVAADDGIKMQTEEAIDHAKAANVPIIVFVNKMDKYEANPEKVLNQL
HHCCCCCEEEEEEEEECCCCCEECHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHH
SAKEIVAEELGGDVVFVKGSALKNEGISELLDSILLIAELNNYKANPNRLAYGTTIEANL
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECC
DKGHGPLATLLVQNGTLRKGDYLVVGSTYGKIRNMFDEYDNEIEIALPSKPVKVSGFEEV
CCCCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCCEEECCCCCC
PTAGDKFLALADEKQARAIANDVKQKKMRLERAMLQSSDIRTKIANGELKNINLIIKADV
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEECC
QGSLEALKGIFSSINIEGVTTTLIRSAIGTISESDVRLAQTSDAIIIGFNVRASRIIKDL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCHHHHHHHHH
ADSVGVQIMNYDIIYKFKEDLELMKGTLDPIIIEEVIGEAKVLKLFKHSQVGTICGCRVI
HHHHCEEEEEEEEEEEEHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEE
NGKIKRNALVRVLRDGIVIYNSKIATLQHNKDSVNEVIADKECGLTIANFNDIKENDIIE
CCHHHHHHHHHHHHCCEEEEECEEEEEECCHHHHHHHHCCCCCCEEEECCCCCCCCCEEE
VYIKVEKKHDEVK
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA