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The map label for this gene is nusA

Identifier: 13357878

GI number: 13357878

Start: 364755

End: 366131

Strand: Direct

Name: nusA

Synonym: UU318

Alternate gene names: 13357878

Gene position: 364755-366131 (Clockwise)

Preceding gene: 13357877

Following gene: 13357879

Centisome position: 48.52

GC content: 27.02

Gene sequence:

>1377_bases
ATGTCTAACTCATTTAAATCAAAAGAATTTATTGAATACTTTAAAGATACAGCAAAACAAAATGAAATTGAATTTGAAGT
TTTATCTTCAATTATTAAAGAAGCATTTGAAAAAGCTTATTTACGAACACATCCTGGTGAAAATTTTGAAACAAATATTG
ATCTTAAACAAGGAACAATTAATTGTTTTCGAAATTTAGTGGTCGTTGAGAATGAAAAAGTACATAATGAAGATTTAGAA
ACATGTTTGGATGATGCTGTTGAAATTTTATTAGATGATGCTCGTAAAATCAATGCAAATGCTCAAATTGGCGATCTTAT
TAAACAACATATTTCAATTGATAATTTTAAAAGTATTGAAGTAGGTCAAATTGGAAGTTTATTGCGTCAAAAAATTACTG
AAATTCATAATAAAAGAGTAGCTGATTTTTGAAAACCATCTTTAATGAAAATGATTCGTGCAAAAGTTGCAGAAGTTAAT
TATAACAAACAACGTAATGAAATTACTGGAGTAAAAGTTGAACTAGATGACCAATGAAAAACATTAGGTTATTTAAGTCG
TAAAGATCGTATTGGTGATGAAAAGTTTAAGGTTGGTGAAACTTATGATTTTCTTATTAAAGAAGTTAAAGAACAAAGCC
GTTTATGGCCAATTTTACTATCTAGAACAGAGCCAGAATTAGTTGAAGAAATTTTAAAACGCGAAGTTGTTGATATTAAA
AATGGAAATATTGAAATCAAAAAAATTGCTCGAATAGCTGGTTTTAAAACTAAGGTAGCTGTAAGCACTAATTTATTAAA
TATTGAACCAGTTGCTGTTGTTGTCGGAAATAAAGGTTTAACTATAACGTCAATTTCTAAACAATTAAATAATGAACGTA
TTGATATTATTCGTTATGCTGATGACAAACGTATTTTTATTGCTAATGCCATTGGTTTAGATAAATTAAAAGGACTTTTA
GTTCAAGAAAATGAAAGTGGTCAACGTTCTGCAATTGCTATTGTAAGCAAGGATGATTTACCAAGTGTTATCGGTCGTGG
TGGAGCAAATATTCGTTTAATTGCTAAAATTACTGAATGAAATATTGATGTAAAAACAATTGAGCAAGCATTCGAAGAAA
ATATTTTATACGAAAAATTTGATGAAAAAATTTATCGTTCATGAAATATTGAATCAATTAATAAAAAAAATGTCACAAAT
GATGAAATGTTAGCTTTAATTGATAGTATAGAAGATGATAAAATTGAACAAGATGTCCAATTAAATCACCCATTAAAACA
ACAAGAAAAAAAAGTTGTTTCTTCAAATGATGAAGATGGTGAAAATGATGAAACACAATTAGAATATCTTGAAGGATTCG
AAGATTTTAAATTCTAA

Upstream 100 bases:

>100_bases
TATTTAGAATGTTATGATATAATTTAACCAATAAGTAAAATGTGAATGAAATGACGACCTTAGGGTTGTCATTTCTTATT
TATATGAAAGGTTATAAAAA

Downstream 100 bases:

>100_bases
TTTAAATATTATGGCAATGAAAGTTAATGAAAGAACTTGTGTTTTTAGTAAAGAAAAATATCCTAAAGATCATTTAGTTC
GCTTTGTTATTATTAACGGT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSNSFKSKEFIEYFKDTAKQNEIEFEVLSSIIKEAFEKAYLRTHPGENFETNIDLKQGTINCFRNLVVVENEKVHNEDLE
TCLDDAVEILLDDARKINANAQIGDLIKQHISIDNFKSIEVGQIGSLLRQKITEIHNKRVADFWKPSLMKMIRAKVAEVN
YNKQRNEITGVKVELDDQWKTLGYLSRKDRIGDEKFKVGETYDFLIKEVKEQSRLWPILLSRTEPELVEEILKREVVDIK
NGNIEIKKIARIAGFKTKVAVSTNLLNIEPVAVVVGNKGLTITSISKQLNNERIDIIRYADDKRIFIANAIGLDKLKGLL
VQENESGQRSAIAIVSKDDLPSVIGRGGANIRLIAKITEWNIDVKTIEQAFEENILYEKFDEKIYRSWNIESINKKNVTN
DEMLALIDSIEDDKIEQDVQLNHPLKQQEKKVVSSNDEDGENDETQLEYLEGFEDFKF

Sequences:

>Translated_458_residues
MSNSFKSKEFIEYFKDTAKQNEIEFEVLSSIIKEAFEKAYLRTHPGENFETNIDLKQGTINCFRNLVVVENEKVHNEDLE
TCLDDAVEILLDDARKINANAQIGDLIKQHISIDNFKSIEVGQIGSLLRQKITEIHNKRVADF*KPSLMKMIRAKVAEVN
YNKQRNEITGVKVELDDQ*KTLGYLSRKDRIGDEKFKVGETYDFLIKEVKEQSRLWPILLSRTEPELVEEILKREVVDIK
NGNIEIKKIARIAGFKTKVAVSTNLLNIEPVAVVVGNKGLTITSISKQLNNERIDIIRYADDKRIFIANAIGLDKLKGLL
VQENESGQRSAIAIVSKDDLPSVIGRGGANIRLIAKITE*NIDVKTIEQAFEENILYEKFDEKIYRS*NIESINKKNVTN
DEMLALIDSIEDDKIEQDVQLNHPLKQQEKKVVSSNDEDGENDETQLEYLEGFEDFKF
>Mature_457_residues
SNSFKSKEFIEYFKDTAKQNEIEFEVLSSIIKEAFEKAYLRTHPGENFETNIDLKQGTINCFRNLVVVENEKVHNEDLET
CLDDAVEILLDDARKINANAQIGDLIKQHISIDNFKSIEVGQIGSLLRQKITEIHNKRVADF*KPSLMKMIRAKVAEVNY
NKQRNEITGVKVELDDQ*KTLGYLSRKDRIGDEKFKVGETYDFLIKEVKEQSRLWPILLSRTEPELVEEILKREVVDIKN
GNIEIKKIARIAGFKTKVAVSTNLLNIEPVAVVVGNKGLTITSISKQLNNERIDIIRYADDKRIFIANAIGLDKLKGLLV
QENESGQRSAIAIVSKDDLPSVIGRGGANIRLIAKITE*NIDVKTIEQAFEENILYEKFDEKIYRS*NIESINKKNVTND
EMLALIDSIEDDKIEQDVQLNHPLKQQEKKVVSSNDEDGENDETQLEYLEGFEDFKF

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=333, Percent_Identity=27.3273273273273, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 51822; Mature: 51690

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSFKSKEFIEYFKDTAKQNEIEFEVLSSIIKEAFEKAYLRTHPGENFETNIDLKQGTI
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHH
NCFRNLVVVENEKVHNEDLETCLDDAVEILLDDARKINANAQIGDLIKQHISIDNFKSIE
HHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
VGQIGSLLRQKITEIHNKRVADFKPSLMKMIRAKVAEVNYNKQRNEITGVKVELDDQKTL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHH
GYLSRKDRIGDEKFKVGETYDFLIKEVKEQSRLWPILLSRTEPELVEEILKREVVDIKNG
HHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHCCCCC
NIEIKKIARIAGFKTKVAVSTNLLNIEPVAVVVGNKGLTITSISKQLNNERIDIIRYADD
CEEHHHHHHHHCCCEEEEEEECEEEECEEEEEECCCCCEEEHHHHHHCCCCEEEEEECCC
KRIFIANAIGLDKLKGLLVQENESGQRSAIAIVSKDDLPSVIGRGGANIRLIAKITENID
CEEEEEECCCHHHHCCEEEECCCCCCCEEEEEEECCCCHHHHCCCCCCEEEEEEECCCCC
VKTIEQAFEENILYEKFDEKIYRSNIESINKKNVTNDEMLALIDSIEDDKIEQDVQLNHP
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHCCCCCCCCC
LKQQEKKVVSSNDEDGENDETQLEYLEGFEDFKF
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNSFKSKEFIEYFKDTAKQNEIEFEVLSSIIKEAFEKAYLRTHPGENFETNIDLKQGTI
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHH
NCFRNLVVVENEKVHNEDLETCLDDAVEILLDDARKINANAQIGDLIKQHISIDNFKSIE
HHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
VGQIGSLLRQKITEIHNKRVADFKPSLMKMIRAKVAEVNYNKQRNEITGVKVELDDQKTL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHH
GYLSRKDRIGDEKFKVGETYDFLIKEVKEQSRLWPILLSRTEPELVEEILKREVVDIKNG
HHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHCCCCC
NIEIKKIARIAGFKTKVAVSTNLLNIEPVAVVVGNKGLTITSISKQLNNERIDIIRYADD
CEEHHHHHHHHCCCEEEEEEECEEEECEEEEEECCCCCEEEHHHHHHCCCCEEEEEECCC
KRIFIANAIGLDKLKGLLVQENESGQRSAIAIVSKDDLPSVIGRGGANIRLIAKITENID
CEEEEEECCCHHHHCCEEEECCCCCCCEEEEEEECCCCHHHHCCCCCCEEEEEEECCCCC
VKTIEQAFEENILYEKFDEKIYRSNIESINKKNVTNDEMLALIDSIEDDKIEQDVQLNHP
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHCCCCCCCCC
LKQQEKKVVSSNDEDGENDETQLEYLEGFEDFKF
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993; 8253680 [H]