Definition Clostridium difficile 630 chromosome, complete genome.
Accession NC_009089
Length 4,290,252

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The map label for this gene is murC

Identifier: 126701142

GI number: 126701142

Start: 4110891

End: 4112243

Strand: Direct

Name: murC

Synonym: CD3518

Alternate gene names: 126701142

Gene position: 4110891-4112243 (Clockwise)

Preceding gene: 126701079

Following gene: 126701167

Centisome position: 95.82

GC content: 31.56

Gene sequence:

>1353_bases
ATGAATATACACTTTATAGGAATTGGCGGTATCAGCATGAGTGCATTAGCTGAAATATGTATCAATAAAGGTTATCAAGT
TTCAGGTTCGGATTCAAACGAATCATACCTATTAGACAAACTTAGGGACCAAGGGGCAACTATTTATATTGGTCAGAAAA
AAGATAATATATCAGATGATGTAAATATGGTTGTTTATACAGCAGCAGTTCATCCAGACAATGAAGAACTTGTGGCTGCT
AAAGAAAAAAATAAATTGGTAATGAACAGAGCGACTTTTTTAGGCCAAATCATGAGGGAATATAAAAATTCTATAGCAGT
TTCTGGAACACATGGAAAAACTTCTACAACATCTATGTTATCTACAATCTTTGAATATGCAGACTTAGACCCAACTATAC
TAGTTGGTGGTAACTTAAGTATGATAGGTGGTAATGTTAAGATAGGTAATTCCAACCACTTTATAACTGAAGCATGTGAA
TATGTAGATAGTTTCCTTAATTTTAACCCAAAGATATCTATAGTTTTAAATGTTGAAGAAGACCATCTTGATTATTTCTC
TGGAATAGATGAAATCAAGGCTTCTTTTAACAAATTTGGAAAATTATTACCCCCAGAAGGCTATTTCATAATAAATGGCG
ATGATGAAAATATTGATGACATATTGTATGATGTTAAAGCTACTATTATAAAATACGGCAGAGATTCTGACAATGATGCT
GTAATAAAAGATATTCACTTTGACAATAGTGGTCATGGAATATTTAGAATAGAATATGAGGGAAGAGATTTAGGAGAATT
TGAACTTTCTGTATATGGTCTTCATAACATATACAATGCTTCTTCTGCCATAATGGCAGCTCTTGTTTCTGGTATTGACT
TAGAGACTATAAGAAAAAATATAAAAATTTATAAAGGTGTCGGAAGAAGATTTGAAACTAAAGGGTATTATAAGAATGCT
TTAGTAGTAGATGATTATGCACATCATCCAACTGAGTTAAAAGCAACTTTAGCTGCTGCCAAAAAATTAAAAAAATCAAC
TTTATGGTGTATCTTCCAGCCACATACTTATACTAGAACAAAATCTCTTTTAGGTGAATTTTCAGAGGCATTCTATGCTG
CTGATAAAGTTATCATAACAGATATATATGCTGCTAGGGAAAAAGACCCAGGAGATATACATTCTAAGGACTTGGTTGAG
AAATTATATCAAAACAATGTAGATGCCATCTATATAAAAGAATTTGAAGATATAGTAAAATACTTACGTGAAAATGTTAA
GGATAATGATTTAGTAATAACTGCTGGTGCTGGTCCAATTTATAAGGTTGCAGACTTGTTGGTTGAAAAATAA

Upstream 100 bases:

>100_bases
ATTATATGCATATTAATTATAAAATATTGAACATTATATTATTATACCACAGAAATAGTTCATATTGTAGGACTTAATTA
AAAAGAAAGGTGGTTTTATT

Downstream 100 bases:

>100_bases
TATATAAAATAATTTAGGCTATCAAATATCTAATTTGATAGCCTTCTTAAAGTCTTATTTCTTTCCAATTTCAAATATCT
CATTTTTAAGGTAATCAAAT

Product: UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: UDP-N-acetylmuramoyl-L-alanine synthetase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQGATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAA
KEKNKLVMNRATFLGQIMREYKNSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENIDDILYDVKATIIKYGRDSDNDA
VIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHNIYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNA
LVVDDYAHHPTELKATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGDIHSKDLVE
KLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK

Sequences:

>Translated_450_residues
MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQGATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAA
KEKNKLVMNRATFLGQIMREYKNSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENIDDILYDVKATIIKYGRDSDNDA
VIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHNIYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNA
LVVDDYAHHPTELKATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGDIHSKDLVE
KLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
>Mature_450_residues
MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQGATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAA
KEKNKLVMNRATFLGQIMREYKNSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENIDDILYDVKATIIKYGRDSDNDA
VIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHNIYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNA
LVVDDYAHHPTELKATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGDIHSKDLVE
KLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK

Specific function: Cell wall formation

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786279, Length=461, Percent_Identity=35.1409978308026, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1790680, Length=356, Percent_Identity=29.4943820224719, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786273, Length=280, Percent_Identity=27.5, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURC_CLOD6 (Q181B7)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001090039.1
- ProteinModelPortal:   Q181B7
- SMR:   Q181B7
- STRING:   Q181B7
- GeneID:   4912965
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD3518
- NMPDR:   fig|1496.1.peg.3092
- eggNOG:   COG0773
- HOGENOM:   HBG476594
- OMA:   YQPHRYS
- ProtClustDB:   PRK00421
- GO:   GO:0005737
- HAMAP:   MF_00046
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR016040
- InterPro:   IPR005758
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01082

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.8

Molecular weight: Translated: 50240; Mature: 50240

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQGATIYIGQKKDNISDD
CEEEEEEECCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCC
VNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYKNSIAVSGTHGKTSTTSML
CEEEEEEEEECCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
STIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACEYVDSFLNFNPKISIVLNVEE
HHHHHHHCCCCEEEECCCEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECH
DHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENIDDILYDVKATIIKYGRDSDNDA
HHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCC
VIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHNIYNASSAIMAALVSGIDLETIRKN
EEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEHHHHHCHHHHHHHHHHHCCCHHHHHHH
IKIYKGVGRRFETKGYYKNALVVDDYAHHPTELKATLAAAKKLKKSTLWCIFQPHTYTRT
HHHEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHH
KSLLGEFSEAFYAADKVIITDIYAAREKDPGDIHSKDLVEKLYQNNVDAIYIKEFEDIVK
HHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHH
YLRENVKDNDLVITAGAGPIYKVADLLVEK
HHHHCCCCCCEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQGATIYIGQKKDNISDD
CEEEEEEECCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCC
VNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYKNSIAVSGTHGKTSTTSML
CEEEEEEEEECCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
STIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACEYVDSFLNFNPKISIVLNVEE
HHHHHHHCCCCEEEECCCEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECH
DHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENIDDILYDVKATIIKYGRDSDNDA
HHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCC
VIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHNIYNASSAIMAALVSGIDLETIRKN
EEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEHHHHHCHHHHHHHHHHHCCCHHHHHHH
IKIYKGVGRRFETKGYYKNALVVDDYAHHPTELKATLAAAKKLKKSTLWCIFQPHTYTRT
HHHEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHH
KSLLGEFSEAFYAADKVIITDIYAAREKDPGDIHSKDLVEKLYQNNVDAIYIKEFEDIVK
HHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHH
YLRENVKDNDLVITAGAGPIYKVADLLVEK
HHHHCCCCCCEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA