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The map label for this gene is topB [H]

Identifier: 126699469

GI number: 126699469

Start: 2151738

End: 2153444

Strand: Direct

Name: topB [H]

Synonym: CD1859

Alternate gene names: 126699469

Gene position: 2151738-2153444 (Clockwise)

Preceding gene: 126699468

Following gene: 126699470

Centisome position: 50.15

GC content: 34.92

Gene sequence:

>1707_bases
ATGAAACTTGTAATAGCAGAAAAGCCAAGTGTTGCAATTTCTATTGCTAAAGTAATTGGAGCAACAAAAAAGAAAGACGG
ATATTATGAGGGAAATGGATACAAGGTAAGTTGGTGTGTTGGACACTTAATTCAGATGGCAAATCCGGAAAGTTACGATG
AAAAGTACGCTAAATGGAATATGGCGGATTTACCGATTATTCCAAGAGAATATAAGTATGAGATTGCAAAGTCCACAAAG
AAACAATTTACGATTCTTAAAAAGTTGATGAATGACAAGGAAATAGACATTGTGATAAATGCTTGCGATGCAGGACGTGA
AGGAGAAGCAATCTTCAGGCTTGTCTATAATCAGGCAAATTGCAAAAAGAAGATGAAACGCCTTTGGATTTCATCAATGG
AAGATAGTGCAATTAAAGAGGGCTTTGATAACCTAAAAGACGGAAGTTTTTACGATAATCTCTTTGAATCTGCACAAGCA
AGAGCTATTGCGGATTGGCTTGTGGGAATGAATTTAAGTAGGCTATACTCGTGCCTATATAAGCAGAATTATTCTGTTGG
CAGAGTACAAACACCAACTCTTGCCATGATTGTAAAAAGAGATGATGAAATAGCAAATTTCAAGAAAGAAAAATATTTTA
CAGTAGAGCTTGATTTAGACGGCTTTTCTCTTTCTACCGATAGAATTGATGATAAGACAGTTACAGAGCAGCTTATCAAT
CTATTAGGTAATTCCATAGAAATTACCGATGTTATTCAAAAGGAAAAGATAACAAAACCTGAGCTGCCATTTGACCTTAC
AACGCTTCAAAGAGAGTGTAATAAGTATTTCAATTATAGTGCAAAGCAGACCTTAGATTATACACAAAGTTTATACGAGA
AAAAACTGATTACCTATCCAAGAACGGACAGTCGTTATCTTACAGAAGATATGATTACAAGTACAATCAACAATATTGTT
GGAAAGCATGATTTTGATACAGGTCGCATTAAGATGGTATTTAATTCTAAAAGGGTTACAGACCATCATGCGATTATACC
GACAGCAAGCAGTATGAATGAGGACTTAGCTTCACTTCCGGAAAGTGAATTGAAGGTATATGAACTTGTTTTAAATAAGC
TACATGCAAGTGTAGGCTATCCTTTAATTGAGAATACTACAAAGATTGTAGCTGTATTTGACGGATTTGAATTTACAAGC
AGTGGAAAGGTGATTGTAGATGAGGGCTTTACCAAATACCTAAAAAAATATATAAAAAAGAAAAATGAAGATACCGTGTT
TCCGGATTTAAAGATTGGTGATAGTCTTGATATTAAGGAGAAAGAGATAAAAGAAAACTACACATCACCGGCTAAACATT
TTACGGAAGATACACTTTTAAAGGATATGGAGCTTGCAGGAAATGACGCACTGGAAAAAGGTGTAGAAGTTGAGCGAAAA
GGACTTGGAACACCTGCAACAAGAGCAGGCATTATTGAAAATTTAATCTTTAAGGGTTTTGTAGAAAGAGATAAGAAAAA
CCTTATTGCTACACACAAAGGAATAAGCCTTGTAACGATTGTCTCAGATACTTTTAAATCAGCAGGTACAACCGCTAAGT
GGGAAATGGAGTTATCGGATATTGCACAAGGCAAAGCTTCAAAGGAAAATTTTTTAAAAGCGATTGAAAACGAGATAAAA
GAATTGGTTTCAACTTATAGCAAGTAA

Upstream 100 bases:

>100_bases
AGTAAACTTGTACCCAAAATTATATAATATTTGGGGCAAAACACAGGGCGAGAGAGTAAAATCTTTCGCCCTCTTATATT
GAAAACAGGAGGAAAACTAT

Downstream 100 bases:

>100_bases
GATAGCACTAATTTTAACATCGAAAAAATGTCCTAAAAATGCTATAATTTTTGTAGGGAAAGTAGGAAATATGGAGGTGT
TTTATGTCGGAAGTATTTAT

Product: conjugative transposon DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKLVIAEKPSVAISIAKVIGATKKKDGYYEGNGYKVSWCVGHLIQMANPESYDEKYAKWNMADLPIIPREYKYEIAKSTK
KQFTILKKLMNDKEIDIVINACDAGREGEAIFRLVYNQANCKKKMKRLWISSMEDSAIKEGFDNLKDGSFYDNLFESAQA
RAIADWLVGMNLSRLYSCLYKQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYFTVELDLDGFSLSTDRIDDKTVTEQLIN
LLGNSIEITDVIQKEKITKPELPFDLTTLQRECNKYFNYSAKQTLDYTQSLYEKKLITYPRTDSRYLTEDMITSTINNIV
GKHDFDTGRIKMVFNSKRVTDHHAIIPTASSMNEDLASLPESELKVYELVLNKLHASVGYPLIENTTKIVAVFDGFEFTS
SGKVIVDEGFTKYLKKYIKKKNEDTVFPDLKIGDSLDIKEKEIKENYTSPAKHFTEDTLLKDMELAGNDALEKGVEVERK
GLGTPATRAGIIENLIFKGFVERDKKNLIATHKGISLVTIVSDTFKSAGTTAKWEMELSDIAQGKASKENFLKAIENEIK
ELVSTYSK

Sequences:

>Translated_568_residues
MKLVIAEKPSVAISIAKVIGATKKKDGYYEGNGYKVSWCVGHLIQMANPESYDEKYAKWNMADLPIIPREYKYEIAKSTK
KQFTILKKLMNDKEIDIVINACDAGREGEAIFRLVYNQANCKKKMKRLWISSMEDSAIKEGFDNLKDGSFYDNLFESAQA
RAIADWLVGMNLSRLYSCLYKQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYFTVELDLDGFSLSTDRIDDKTVTEQLIN
LLGNSIEITDVIQKEKITKPELPFDLTTLQRECNKYFNYSAKQTLDYTQSLYEKKLITYPRTDSRYLTEDMITSTINNIV
GKHDFDTGRIKMVFNSKRVTDHHAIIPTASSMNEDLASLPESELKVYELVLNKLHASVGYPLIENTTKIVAVFDGFEFTS
SGKVIVDEGFTKYLKKYIKKKNEDTVFPDLKIGDSLDIKEKEIKENYTSPAKHFTEDTLLKDMELAGNDALEKGVEVERK
GLGTPATRAGIIENLIFKGFVERDKKNLIATHKGISLVTIVSDTFKSAGTTAKWEMELSDIAQGKASKENFLKAIENEIK
ELVSTYSK
>Mature_568_residues
MKLVIAEKPSVAISIAKVIGATKKKDGYYEGNGYKVSWCVGHLIQMANPESYDEKYAKWNMADLPIIPREYKYEIAKSTK
KQFTILKKLMNDKEIDIVINACDAGREGEAIFRLVYNQANCKKKMKRLWISSMEDSAIKEGFDNLKDGSFYDNLFESAQA
RAIADWLVGMNLSRLYSCLYKQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYFTVELDLDGFSLSTDRIDDKTVTEQLIN
LLGNSIEITDVIQKEKITKPELPFDLTTLQRECNKYFNYSAKQTLDYTQSLYEKKLITYPRTDSRYLTEDMITSTINNIV
GKHDFDTGRIKMVFNSKRVTDHHAIIPTASSMNEDLASLPESELKVYELVLNKLHASVGYPLIENTTKIVAVFDGFEFTS
SGKVIVDEGFTKYLKKYIKKKNEDTVFPDLKIGDSLDIKEKEIKENYTSPAKHFTEDTLLKDMELAGNDALEKGVEVERK
GLGTPATRAGIIENLIFKGFVERDKKNLIATHKGISLVTIVSDTFKSAGTTAKWEMELSDIAQGKASKENFLKAIENEIK
ELVSTYSK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=613, Percent_Identity=24.6329526916803, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI10835218, Length=601, Percent_Identity=21.9633943427621, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1788061, Length=608, Percent_Identity=32.4013157894737, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1787529, Length=584, Percent_Identity=25.6849315068493, Blast_Score=151, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI32563869, Length=629, Percent_Identity=25.9141494435612, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=610, Percent_Identity=23.6065573770492, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323263, Length=419, Percent_Identity=26.2529832935561, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24640096, Length=618, Percent_Identity=25.0809061488673, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585251, Length=599, Percent_Identity=22.5375626043406, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 64527; Mature: 64527

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVIAEKPSVAISIAKVIGATKKKDGYYEGNGYKVSWCVGHLIQMANPESYDEKYAKWN
CEEEEECCCCHHHHHHHHHCCCCCCCCEECCCCEEEHHHHHHHHHHCCCCHHHHHHHCCC
MADLPIIPREYKYEIAKSTKKQFTILKKLMNDKEIDIVINACDAGREGEAIFRLVYNQAN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
CKKKMKRLWISSMEDSAIKEGFDNLKDGSFYDNLFESAQARAIADWLVGMNLSRLYSCLY
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYFTVELDLDGFSLSTDRIDDKTVTEQLIN
HCCCCCCCCCCCEEEEEEECCCHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHH
LLGNSIEITDVIQKEKITKPELPFDLTTLQRECNKYFNYSAKQTLDYTQSLYEKKLITYP
HHCCCEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
RTDSRYLTEDMITSTINNIVGKHDFDTGRIKMVFNSKRVTDHHAIIPTASSMNEDLASLP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCHHHCCHHHHHCC
ESELKVYELVLNKLHASVGYPLIENTTKIVAVFDGFEFTSSGKVIVDEGFTKYLKKYIKK
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEECCCCCEEECCCHHHHHHHHHHC
KNEDTVFPDLKIGDSLDIKEKEIKENYTSPAKHFTEDTLLKDMELAGNDALEKGVEVERK
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHH
GLGTPATRAGIIENLIFKGFVERDKKNLIATHKGISLVTIVSDTFKSAGTTAKWEMELSD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEHHHHHCCCCCEEEEEEHHH
IAQGKASKENFLKAIENEIKELVSTYSK
HHCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLVIAEKPSVAISIAKVIGATKKKDGYYEGNGYKVSWCVGHLIQMANPESYDEKYAKWN
CEEEEECCCCHHHHHHHHHCCCCCCCCEECCCCEEEHHHHHHHHHHCCCCHHHHHHHCCC
MADLPIIPREYKYEIAKSTKKQFTILKKLMNDKEIDIVINACDAGREGEAIFRLVYNQAN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
CKKKMKRLWISSMEDSAIKEGFDNLKDGSFYDNLFESAQARAIADWLVGMNLSRLYSCLY
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYFTVELDLDGFSLSTDRIDDKTVTEQLIN
HCCCCCCCCCCCEEEEEEECCCHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHH
LLGNSIEITDVIQKEKITKPELPFDLTTLQRECNKYFNYSAKQTLDYTQSLYEKKLITYP
HHCCCEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
RTDSRYLTEDMITSTINNIVGKHDFDTGRIKMVFNSKRVTDHHAIIPTASSMNEDLASLP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCHHHCCHHHHHCC
ESELKVYELVLNKLHASVGYPLIENTTKIVAVFDGFEFTSSGKVIVDEGFTKYLKKYIKK
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEECCCCCEEECCCHHHHHHHHHHC
KNEDTVFPDLKIGDSLDIKEKEIKENYTSPAKHFTEDTLLKDMELAGNDALEKGVEVERK
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHH
GLGTPATRAGIIENLIFKGFVERDKKNLIATHKGISLVTIVSDTFKSAGTTAKWEMELSD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEHHHHHCCCCCEEEEEEHHH
IAQGKASKENFLKAIENEIKELVSTYSK
HHCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA