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The map label for this gene is ogt2 [H]

Identifier: 126699379

GI number: 126699379

Start: 2051781

End: 2052275

Strand: Direct

Name: ogt2 [H]

Synonym: CD1770

Alternate gene names: 126699379

Gene position: 2051781-2052275 (Clockwise)

Preceding gene: 126699378

Following gene: 126699380

Centisome position: 47.82

GC content: 25.05

Gene sequence:

>495_bases
ATGAGTTTAGTATATGATGTTTATAAATCAAAATTAGGAAATTTGTACATATTAAGTGAAGATGGTTTTATAGTTAGTAT
ATACATAGGAGATGAAAAATTTAATAATTTAAAAAATGAATGTATTAAAAAAGGAATTGATATAAAGAGAAGTAAGGAAG
ATTTAAAGGAAGCAATAAGTCAGATAGATGATTACTTTAATGGAAAAAGAAAAGTCTTTAATTTAAATATTCGTATTGAA
GGGACTGAATTTCAAAAATCAGTTTATAGGGAAATGTTAAAAATACCTTATGGAGAAACATTGTGTTATTCAGATATAGC
AAGAAATATAAACAATCCAAAATCAGTAAGAGCAATAGGACAAGCAAGTAAAAGTAATAAAATTCCAATAATAATACCTT
GTCATAGAGTAGTTGGCAAAAAAGATATTGGTGGATATATGGGGAATCATTCTGATTTGAAAGAGATACTATTAAATTTA
GAAAGACAGAGTTAA

Upstream 100 bases:

>100_bases
TATATTAAGTATCTATAAAAAAGAGTTATCCTATTTTTTAGGGTAACTCTTTATTAGTTAAAAAATTATATGTATACTGA
TATTAAAGGGGGGAAGTAAT

Downstream 100 bases:

>100_bases
CTTAGAAAGAAATGTTTTTATTGTTAATATTTGATATAATAATAGTAAAAAGAAGTATTTTTTACAAAAACAATGAAGAA
TTTATTTTAATTTACAAAAA

Product: methylated-DNA--protein-cysteine methyltransferase 2

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKKGIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIE
GTEFQKSVYREMLKIPYGETLCYSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
ERQS

Sequences:

>Translated_164_residues
MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKKGIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIE
GTEFQKSVYREMLKIPYGETLCYSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
ERQS
>Mature_163_residues
SLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKKGIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEG
TEFQKSVYREMLKIPYGETLCYSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNLE
RQS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=160, Percent_Identity=30.625, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1787596, Length=172, Percent_Identity=29.6511627906977, Blast_Score=76, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533068, Length=66, Percent_Identity=50, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115533070, Length=66, Percent_Identity=50, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=40, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18784; Mature: 18653

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKKGIDIKRSKEDLKEAIS
CCHHHHHHHHHCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLCYSDIARNINNPKSVRAIG
HHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHH
QASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNLERQS
HCCCCCCCCEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKKGIDIKRSKEDLKEAIS
CHHHHHHHHHCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLCYSDIARNINNPKSVRAIG
HHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHH
QASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNLERQS
HCCCCCCCCEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]