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The map label for this gene is grdF [H]

Identifier: 126699348

GI number: 126699348

Start: 2017332

End: 2018378

Strand: Direct

Name: grdF [H]

Synonym: CD1741

Alternate gene names: 126699348

Gene position: 2017332-2018378 (Clockwise)

Preceding gene: 126699347

Following gene: 126699349

Centisome position: 47.02

GC content: 34.1

Gene sequence:

>1308_bases
ATGAGTAAAATCAGAGTGGTTCATTATATAAACAATTTCTTTGCTGGTATAGGTGGAGAGGAAAAAGCAGATATTCCGCC
AGAAAAAAGGGCTGGTGTAGTAGGACCTGGAATAGCATTTCAGAGTCAATTTAATGATGATGCAGAGATAATCTCAACTG
TAATTTGCGGAGATACGTATTTTGGTGAAAATATTGAGAGTGCAACTCAAGAACTTTTAAAAATGATAAAAGAAGAAAGC
CCTGATTTATTTATTGCAGGCCCAGCATTTAATGCAGGTAGATATGGAGTAGCTTGTGGGACTATTTGTAAGGCAGTTGA
AGAAGAGTTAAATATTCCTGTAATTACAGGCATGTATAAAGAAAATCCAGGTGTAGATATGTTTAAATTAGATTTGCATA
TAGTGTCTACAGGAAATTCAGCAGCAAGTCTTAGAAAGGTAGTTCCAATAATGACAAAACTAGGTCTTAAATTGGTTAGA
GGTGAGGAAATAGGACCTCCTGAAGAGGAAGGATATATAATGAGAGGAATAAGAAAAAATTTCTTCCATGAACTTAGAGG
GTCTGAAAGAGCAATTGATATGTTGGTTAAAAAGATGAAAGGGGAAAGCTTTGAAACTGAGTATCCAATGCCTGAATTTG
ATAGAGTTACACCTATGAAACCTGTTAAAGATTTATCTAAGATAAAACTTGCCCTAGTAACTTCTGGAGGGATAGTTCCA
ATTGATAATCCAGACAAAATTGAATCTTCAAGTGCTACTAAATATGGAATTTACGATTTAACAGATATGGATTCTATGTC
AAATAAAGAATTTACAACTATACATGGGGGGTATGACAGAGCATATGTCCTTGAAAATCCAAATTTAGTTGTACCACTAG
ATGTAGTTAGAGAGCTTGAAAAAGAAGGTGTAATTGGAGAATTAGTAGATTATTTTATAACTACTACAGGAACAGGAACG
AGTGTTGGCAATTCTAAGAGATTTGGAGAAGAGTTTTCTAAGAAATTGTTAGAAGATGATGTAGATGCTGTAATTTTAAC
TTCTACATGAGGTACTTGTACTCGTTGTGGAGCAACGATGGTAAAAGAGATTGAGAGAGCTGGTATAGCAGTTGTTCATA
TATGTACAGTAGTACCAATATCACTTACAATAGGAGCTAATAGAATTGTTCCAGCAGTAGGGATACCATATCCACTTGGA
AATCCTATGTTAGGAGAAGAGGAAAGTAAAAAAATTAGAAAGAATATAGTTTTAAAAGCTTTAAATGCACTAACAGAAGA
TATAGAAGAACAAACAGTATTTGAATAA

Upstream 100 bases:

>100_bases
GCAAGATATTATTAAATAAGTATTTGAATATAAAGTTCTGTTGATGTAATCATTGGTCTAACTGATGTAATAACTATAAA
GTGATTTGGAGGATTAAGAG

Downstream 100 bases:

>100_bases
TAAAAATACAACACAACACTGAAATAAAAAAAGATATCAAAAAATAATTTTTTAAAATTATTTTACGATATCTTTTTTTT
ATTAGGTAAATTCGAAATTT

Product: sarcosine reductase complex component B subunit alpha

Products: NA

Alternate protein names: Selenoprotein PB beta [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>434_residues
MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQFNDDAEIISTVICGDTYFGENIESATQELLKMIKEES
PDLFIAGPAFNAGRYGVACGTICKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTPMKPVKDLSKIKLALVTSGGIVP
IDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHGGYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGT
SVGNSKRFGEEFSKKLLEDDVDAVILTSTGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPAVGIPYPLGN
PMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE

Sequences:

>Translated_349_residues
MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQFNDDAEIISTVICGDTYFGENIESATQELLKMIKEES
PDLFIAGPAFNAGRYGVACGTICKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTPMKPVKDLSKIKLALVTSGGIVP
IDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHGGYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGT
SVGNSKRFGEEFSKKLLEDDVDAVILTST
>Mature_348_residues
SKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQFNDDAEIISTVICGDTYFGENIESATQELLKMIKEESP
DLFIAGPAFNAGRYGVACGTICKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVRG
EEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTPMKPVKDLSKIKLALVTSGGIVPI
DNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHGGYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTS
VGNSKRFGEEFSKKLLEDDVDAVILTST

Specific function: In the first step of sarcosine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB-activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated selen

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grdB/grdF/grdH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010186
- InterPro:   IPR022787
- InterPro:   IPR010187 [H]

Pfam domain/function: PF07355 GRDB [H]

EC number: =1.21.4.3 [H]

Molecular weight: Translated: 38387; Mature: 38256

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00104 EPSP_SYNTHASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQFNDDAEIISTVICGDTY
CCCEEEEHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCEECCCCCCHHHHHHHHHHCCCC
FGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTICKAVEEELNIPVITGMYK
CCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEC
ENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVRGEEIGPPEEEGYIMRGIRKN
CCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHH
FFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTPMKPVKDLSKIKLALVTSGGIVP
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCEEE
IDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHGGYDRAYVLENPNLVVPLDVVRELE
CCCCCCCCCCCCCEECEEECCCCCCCCCCCEEEEECCCCEEEEECCCCEEEEHHHHHHHH
KEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSKKLLEDDVDAVILTST
HCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
SKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQFNDDAEIISTVICGDTY
CCEEEEHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCEECCCCCCHHHHHHHHHHCCCC
FGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTICKAVEEELNIPVITGMYK
CCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEC
ENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVRGEEIGPPEEEGYIMRGIRKN
CCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHH
FFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTPMKPVKDLSKIKLALVTSGGIVP
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCEEE
IDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHGGYDRAYVLENPNLVVPLDVVRELE
CCCCCCCCCCCCCEECEEECCCCCCCCCCCEEEEECCCCEEEEECCCCEEEEHHHHHHHH
KEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSKKLLEDDVDAVILTST
HCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA