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The map label for this gene is aspB [H]

Identifier: 126699141

GI number: 126699141

Start: 1782581

End: 1783975

Strand: Direct

Name: aspB [H]

Synonym: CD1537

Alternate gene names: 126699141

Gene position: 1782581-1783975 (Clockwise)

Preceding gene: 126699140

Following gene: 126699142

Centisome position: 41.55

GC content: 34.55

Gene sequence:

>1395_bases
ATGGATGCTAAAAAAGTAAAAGTACCAGTTAGAGAACAAGAACCTGCTGTGAGAGCAACAAATTTTGATGAGGTATGCTT
AGGGTATAATAAAGAAGAAGCTATGGCAGAAGCTAATAGATGTTTAGCTTGTAAAAAACCAAAATGTGTGGGTGGATGTC
CAGTAGGAATCGACATACCAGGGTTTATAACAAAAATTAAAGAAGATGATATAGAAGGTGCTGCTAAAGTAATAGCTAAG
AGCAGTTCATTACCAGCTGTTTGTGGTAGAGTATGCCCACAAGAAAGTCAATGTGAAGGTGTATGTATACTTGGAATAAA
ATCAGATGCTGTATCAATAGGTAAATTAGAAAGATTTGTAGCAGATTGGTCAAAAGAAAATGACATAAATTTATCTGATA
CAGAGCCTAAGAAAAATCAAAAAGTAGCAGTAATAGGAAGTGGTCCAGCAGGTCTAGCTTGTGCTGGTGATTTAGCTAAA
AAAGGCTATGATGTAACAATCTTTGAAGCAATGCATGAGCCAGGTGGAGTTTTAACTTACGGAATACCTGAGTTTAGACT
TCCAAAACAAGCTGTTGTTCAACCTGAAATAGACAATATAAGAAAATTAGGTGTAAAAATAGAAACTAATGTAATTGTTG
GTAAAACAATTACAGTAGATGAACTTATAGAAGATGAAGGATTTGAAGCTATATTCATAGGTTCAGGAGCTGGTCTTCCA
ATGTTTATGAATATACCAGGGGAAAATGCAAATGGAGTATTTTCTGCAAATGAGTTTTTAACTAGAGTAAACTTAATGAA
GGCTTATAGAGATGATTATGATACACCTATTAGTTCTGGTAAAAAAGTTGCTGTAGTAGGTGGTGGAAATGTTGCTATGG
ACGCTGCTAGAACTGCATTAAGGTTAGGTTCAGAATCATATATAGTTTATAGAAGAAGTGAAAAAGAGCTTCCAGCAAGA
GCTGAAGAAGTTCATCATGCAAAAGAAGAAGGCATTATATTTAATACATTAACAAATCCTAAGGAAATTTTAGTAGATGA
AAATGGATATGTTAAAGGTATGGTTTGTATAAGAATGGAATTAGGAGAGCCAGATGATTCTGGAAGAAGAAGACCTATAG
AAATAGAAGGTTCTGAATTTGTACTTGATGTAGACACAGTTATAATGTCACTTGGAACAAGCCCAAATCCACTAATATCT
TCTACAACAAAGAGTCTTGATATAAATAAGAAGAGATGCTTAATAACAGATGAAAATGGTCAGACTTCTAAAGAGGGTGT
ATTTGCAGGTGGAGATGCTGTTACTGGAGCAGCAACAGTAATATCTGCAATGGGTGCTGGTAAAACAGCAGCAGCATCAA
TAGATGAATATTTAAAAGCAAAAGTTAATGCTTAA

Upstream 100 bases:

>100_bases
GACAGTCTATGTACAAGACTCAAGAGGGAAGAGCTATGCTAAAACTTGAAGAAGGCGATAGTCATCATCATAGTAATTGT
GGATGTGGAGGTAACAAATA

Downstream 100 bases:

>100_bases
TTTGTAAATTAAAGAGAGAGTGTCTCAAAATGAACTTTTTAGTTCATGAGGCACTCTTTTTGTACAAAATCAAATATGTT
TTAATCATTTCAACAATGAA

Product: oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLACKKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAK
SSSLPAVCGRVCPQESQCEGVCILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTITVDELIEDEGFEAIFIGSGAGLP
MFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTPISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPAR
AEEVHHAKEEGIIFNTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLGTSPNPLIS
STTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTAAASIDEYLKAKVNA

Sequences:

>Translated_464_residues
MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLACKKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAK
SSSLPAVCGRVCPQESQCEGVCILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTITVDELIEDEGFEAIFIGSGAGLP
MFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTPISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPAR
AEEVHHAKEEGIIFNTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLGTSPNPLIS
STTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTAAASIDEYLKAKVNA
>Mature_464_residues
MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLACKKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAK
SSSLPAVCGRVCPQESQCEGVCILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTITVDELIEDEGFEAIFIGSGAGLP
MFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTPISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPAR
AEEVHHAKEEGIIFNTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLGTSPNPLIS
STTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTAAASIDEYLKAKVNA

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=471, Percent_Identity=33.9702760084926, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI237757300, Length=106, Percent_Identity=36.7924528301887, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788811, Length=455, Percent_Identity=38.2417582417582, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI87082180, Length=461, Percent_Identity=37.9609544468547, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=36.1111111111111, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1788468, Length=441, Percent_Identity=33.3333333333333, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1789243, Length=421, Percent_Identity=33.2541567695962, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI71984108, Length=450, Percent_Identity=33.1111111111111, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17570289, Length=490, Percent_Identity=29.1836734693878, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17543860, Length=178, Percent_Identity=32.0224719101124, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=473, Percent_Identity=30.2325581395349, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=29.8353909465021, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=29.8353909465021, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI28574881, Length=483, Percent_Identity=29.6066252587992, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24665539, Length=483, Percent_Identity=29.6066252587992, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24640763, Length=442, Percent_Identity=32.579185520362, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI18858217, Length=442, Percent_Identity=32.579185520362, Blast_Score=182, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 49596; Mature: 49596

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLACKKPKCVGGCPVGIDIP
CCCCEEECCCCCCCCCEECCCCCHHHCCCCHHHHHHHHHHCEECCCCCCCCCCCCCCCCC
GFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVCILGIKSDAVSIGKLERFV
HHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
ADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAKKGYDVTIFEAMHEPGGVLTY
HHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCHHHHCCCCEEEEEECCCCCCEEEE
GIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTITVDELIEDEGFEAIFIGSGAGLP
CCCCCCCCHHHCCCCCHHHHHHCCEEEECCEEEECEEEHHHHHHCCCCCEEEEECCCCCC
MFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTPISSGKKVAVVGGGNVAMDAARTAL
EEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEHHHHHHHH
RLGSESYIVYRRSEKELPARAEEVHHAKEEGIIFNTLTNPKEILVDENGYVKGMVCIRME
HCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCEEEECCCCCEEEEEEEEEE
LGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLGTSPNPLISSTTKSLDINKKRCLITDENG
CCCCCCCCCCCCEEECCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
QTSKEGVFAGGDAVTGAATVISAMGAGKTAAASIDEYLKAKVNA
CCCCCCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLACKKPKCVGGCPVGIDIP
CCCCEEECCCCCCCCCEECCCCCHHHCCCCHHHHHHHHHHCEECCCCCCCCCCCCCCCCC
GFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVCILGIKSDAVSIGKLERFV
HHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
ADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAKKGYDVTIFEAMHEPGGVLTY
HHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCHHHHCCCCEEEEEECCCCCCEEEE
GIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTITVDELIEDEGFEAIFIGSGAGLP
CCCCCCCCHHHCCCCCHHHHHHCCEEEECCEEEECEEEHHHHHHCCCCCEEEEECCCCCC
MFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTPISSGKKVAVVGGGNVAMDAARTAL
EEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEHHHHHHHH
RLGSESYIVYRRSEKELPARAEEVHHAKEEGIIFNTLTNPKEILVDENGYVKGMVCIRME
HCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCEEEECCCCCEEEEEEEEEE
LGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLGTSPNPLISSTTKSLDINKKRCLITDENG
CCCCCCCCCCCCEEECCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
QTSKEGVFAGGDAVTGAATVISAMGAGKTAAASIDEYLKAKVNA
CCCCCCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]