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The map label for this gene is rpoD2 [H]

Identifier: 126699102

GI number: 126699102

Start: 1734991

End: 1735881

Strand: Direct

Name: rpoD2 [H]

Synonym: CD1498

Alternate gene names: 126699102

Gene position: 1734991-1735881 (Clockwise)

Preceding gene: 126699101

Following gene: 126699103

Centisome position: 40.44

GC content: 29.41

Gene sequence:

>891_bases
TTGATTATGGATACTAAAAAAGAAAATAATTATGAAAATTATATTTGTGTGGGTTCATCAAATGTATCAAATGCGATGAA
AATGTATTTAAAGGAAATAGAGGAATACAAAATGTTGTCAGCAGGAGAAGAGGTAGAGCTGGCTAAGGAAATAATCAACT
CATCTTCAGTAGCAAAAGAAAAATTTATAAATTCAAATTATAGGTTGGTAGTGAGCATAGCAAAGAGATATAAAAGAGAT
AGTATTGATATGTTGGATTTAATTCAAGCAGGAAATATAGGACTTATAAAAGCTGTAGAAAAGTACGATTATAAAAAAGG
ATACAAATTTAGCACTTATGCAACTTGGTGGATAAAACAAAGTATAACAAGATATATCGACGATTGCGAAAATACTATTA
GGATACCTATACATCTTCATCAGAGAATAAATTTTGTAAAGAAAAAGAAACAAGAACTTTTAAATGTATTATTAAGAGAG
CCAACTATAGATGAGATTGCAGATGCATGTGGTTTAGAAGTTGACAAAGTGTTGGAATTATTAAGAAGAGATAAAAATGT
TGTATCTTTGGATACTCCATTAAAAGAAGATGAGGATAGTAGTTTAGTTGAATTTATACCATCAGATGCTGATTTTAAAG
ATGTTGTAATACACGAAGTTGAACAGCATAATTTAAAAGAAAAGATAGAAGAACTACTTACAGGTCTTGGAGAGCAAGAA
CAGCAAGTTTTAAGAATGAGATTTGGAATTGATGATGATGACCCTAAAACACTAGAACAGATTGGAAAGGTATTTGGAGT
TACCAGAGAACGAATAAGACAGATAGAAGCTAAAGCAATAAGAAAATTAAGACATCCAAGTAAGTTAAAACAATTAAAGC
ATTTTTATTAA

Upstream 100 bases:

>100_bases
CAAAACAATTCATTTAAAGAATGGGATATTATGTAATTGTAGATTATGTAGGTTTAAATTATTTATTTTTGTTTTAGCAA
ATTCTATGAATAGAAGGGGT

Downstream 100 bases:

>100_bases
TATTTTTAGACACATTATATATGAGAGTATGAGAAAGCATGTTAATCTCTACTTAGGAATTATTTTTTTATAAAATCTGT
TTATGAACAGCACACTTGTA

Product: RNA polymerase sigma factor rpoD

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MIMDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVELAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRD
SIDMLDLIQAGNIGLIKAVEKYDYKKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLRE
PTIDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEVEQHNLKEKIEELLTGLGEQE
QQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAKAIRKLRHPSKLKQLKHFY

Sequences:

>Translated_296_residues
MIMDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVELAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRD
SIDMLDLIQAGNIGLIKAVEKYDYKKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLRE
PTIDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEVEQHNLKEKIEELLTGLGEQE
QQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAKAIRKLRHPSKLKQLKHFY
>Mature_296_residues
MIMDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVELAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRD
SIDMLDLIQAGNIGLIKAVEKYDYKKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLRE
PTIDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEVEQHNLKEKIEELLTGLGEQE
QQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAKAIRKLRHPSKLKQLKHFY

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=273, Percent_Identity=45.7875457875458, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1789098, Length=275, Percent_Identity=39.2727272727273, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789871, Length=265, Percent_Identity=29.0566037735849, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1788231, Length=214, Percent_Identity=28.5046728971963, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001909
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34548; Mature: 34548

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVELAKEIINSSSVAKE
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
KFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDYKKGYKFSTYATWWIKQ
HHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
SITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPTIDEIADACGLEVDKVLEL
HHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
LRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEVEQHNLKEKIEELLTGLGEQE
HHCCCCEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAKAIRKLRHPSKLKQLKHFY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MIMDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVELAKEIINSSSVAKE
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
KFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDYKKGYKFSTYATWWIKQ
HHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
SITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPTIDEIADACGLEVDKVLEL
HHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
LRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEVEQHNLKEKIEELLTGLGEQE
HHCCCCEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAKAIRKLRHPSKLKQLKHFY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA