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The map label for this gene is sphR [H]

Identifier: 126699068

GI number: 126699068

Start: 1692377

End: 1693081

Strand: Direct

Name: sphR [H]

Synonym: CD1464

Alternate gene names: 126699068

Gene position: 1692377-1693081 (Clockwise)

Preceding gene: 126699067

Following gene: 126699069

Centisome position: 39.45

GC content: 27.38

Gene sequence:

>705_bases
ATGTTAAAGTTGTTGATAGTTGAAGATGATAGTACTATATCTTTTGGAATAAAGTATGCTTTAGAACAAGAAGGATTTAG
TATAGATATATCAAAGGATTTATCTAGTGGAAAAGAAATGATTTCTTCTAATGAATATAGCATGATACTGTTGGATGTTA
CATTGCCAGATGGAACTGGTTATGAGCTTTGTCAATATATAAGAGGATTCTCTCAGGTTCCTATAATATTCTTGACTGCT
TGTGATGAAGAAGTAAATATTGTAATGGGTCTCGATATAGGTGGAGATGATTATATAACAAAACCTTTTAGAATTAGAGA
ATTAATATCCAGAATAAAAGCTGTTTTAAGAAGAAAAGGTAATACTTCAGAAGAAAATAAGAAAATACTTAAATTTGGAG
ATTTAAGTATATATACATTAGAAGCTAGAGTATATAAAAATGATAAAGAAATATTTCTGACATCAGTTGAATATAAACTT
CTTTTAATTCTTATACAGAATAAAAATACTGTTTTAAGTAGAACTCAAATATTGGAAAAACTTTGGGATGTAACTTATGA
TTTTGTAAACGACAATACTCTAACTGTTTATATAAAAAGGCTTAGAGAAAAAATAGGTGATGATTTATGTGAACCAATCT
ACATAGAAACTGTAAGGGGGATTGGTTATAAATGGATAGGAAGTGAAAATAGTGTTTCTATATAA

Upstream 100 bases:

>100_bases
TTAGTATCCAGGGTAATTGTGTATAATAAAACTTGTATAGATAGATAGTATGAAATTAAGCAGATAGTTTAAAATTAAAA
TGAAAAGAAAGGAAATAGTT

Downstream 100 bases:

>100_bases
TCCAGAAGTGAAGAAGTTTTTAAGTAAGTATGTTACTTTGATGTTTATTGTTATCATAATATCTATTGGATTTAGCATAA
TCAACGTGTCCTTAACTAAA

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTA
CDEEVNIVMGLDIGGDDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKL
LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETVRGIGYKWIGSENSVSI

Sequences:

>Translated_234_residues
MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTA
CDEEVNIVMGLDIGGDDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKL
LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETVRGIGYKWIGSENSVSI
>Mature_234_residues
MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTA
CDEEVNIVMGLDIGGDDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKL
LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETVRGIGYKWIGSENSVSI

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786911, Length=227, Percent_Identity=35.2422907488987, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=34.9775784753363, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=31.8777292576419, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=34.9344978165939, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI87082012, Length=226, Percent_Identity=32.3008849557522, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790552, Length=234, Percent_Identity=34.6153846153846, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=34.5132743362832, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=36.283185840708, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=34.070796460177, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=30.1310043668122, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=30.7359307359307, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=30.1310043668122, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=30.3571428571429, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=29.3103448275862, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26813; Mature: 26813

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSNEYSMILLDVTLPDGTG
CEEEEEEECCCEEEEEEEEEEECCCCEEEEHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
YELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKPFRIRELISRIKAVLRRKG
HHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
NTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKLLLILIQNKNTVLSRTQILEK
CCCCCCCEEEEECCEEEEEEEEEEEECCCEEEEEEECEEEEEEEEECCCCHHHHHHHHHH
LWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETVRGIGYKWIGSENSVSI
HHHCEEECCCCCCHHHHHHHHHHHHCHHHCCCCEEEHHHCCCEEEECCCCCCCC
>Mature Secondary Structure
MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSNEYSMILLDVTLPDGTG
CEEEEEEECCCEEEEEEEEEEECCCCEEEEHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
YELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKPFRIRELISRIKAVLRRKG
HHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
NTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKLLLILIQNKNTVLSRTQILEK
CCCCCCCEEEEECCEEEEEEEEEEEECCCEEEEEEECEEEEEEEEECCCCHHHHHHHHHH
LWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETVRGIGYKWIGSENSVSI
HHHCEEECCCCCCHHHHHHHHHHHHCHHHCCCCEEEHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]