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The map label for this gene is dnaG [H]

Identifier: 126699058

GI number: 126699058

Start: 1682862

End: 1684688

Strand: Direct

Name: dnaG [H]

Synonym: CD1454

Alternate gene names: 126699058

Gene position: 1682862-1684688 (Clockwise)

Preceding gene: 126699057

Following gene: 126699059

Centisome position: 39.23

GC content: 26.44

Gene sequence:

>1827_bases
ATGTTAACACAAAAAATTACACCAGAAGGTGATAATATGAATGATATAAAAGACATTATTGAAGAAATAAAAGCTAGATG
CGATATAGCAAGTATAATATCTGAATATATGAATATAAAACAATCTGGAGCTAATTACAAAGGATTATGTCCATTTCATG
GCGAAAAGACACCTTCTTTTTATATAAATACGTCAAAACAGATTTATAAGTGTTTTGGTTGTGGTGAAGGTGGAGACGTA
ATAAATTTTGTCATGAAAATGGAAAATTTGGATTTTATGGATGCTGTAAAAATTTTAGCAAATAAATGCGGTATAGAAAT
TAACACTAACATGAATGAAGAAACTAGGATTAAAATAGAAAAATCTAAGAAATTTCAAGATATTCATACGGAAGCAGCTA
GATTTTATCTTTCAAACTTATTGGGAAGCAAAAATCTTGGGTATGAATATTTAAGAATAAGAGGACTAGATGATAAAATT
ATTAAGAAATTTGGTTTAGGATTTTCTTTGGATTCATGGAATTCACTTATGAATACTCTAATCAGTAAAGGATATAAAAA
ACAAGACTTATTGGAGTGCGGTCTAATTGCAAAAAATAGAGATGGAACTAATTGCTATGATAAATTTAGAAATAGAGTTA
TGTTTCCTATATTCGATTATAGAGGAAATATAATAGGATTTGGGGGAAGAGTATTAGACGATTCTTTGCCAAAATACTTA
AATTCTCCAGACACATTAATTTTTAATAAAAAACAGAACTTATACGGATTAAATTTTGCTAGAAAAAATTTAGAAAGTAA
AACTATAGTCTTAGTTGAAGGATATATGGATTTAATTTCACTTTATCAGTATGGTATAAAAAATGTAGTAGCAACCCTTG
GAACAGCCTTGACTGAACAACAAGGCATTTTAATAAAGAGATATGCGGACACTGCTATTATATCTTATGATTCTGATGAA
GCAGGAATAAAAGCGACTTTAAGAGCTATAGATATACTTACTAAATTAGGTATCAATGTAAAAGTGTTAGACTTAAAAGA
TGCTAAAGACCCAGATGAATTTGTAAGAAAATACGGACTTAGTGATTATAAAAAAGCTATGGATGTTTCTACTCACTACA
TAAAATATAAAATAGATCATCTTAAAAAAGAATTTAATATTCAAAAAGATGAAGAACGAGTGAAGTTCGCAAAAGAAGCT
TCTAAAATTATAAAACAATTGACAAGTCCTGTTGAAATTGATTTTTATACAAAGTATTTAAGCAATCAAATAGATATTAA
TGTCGAGTCTATAAAGAGAGAAGTTTATGGTAAAAATTACAATAAGCCATATAATAACAAAAATCAAAAGAAAATAGAAG
AAAAAGTAATTGAAAAAGTCGAAGTCAGACAAGACGGAAAACAATTAGTTGAAGAAACTCTAATAAAAATAATGTTAGAA
GACAAAAAAATTAGAGAAATAGCATTACTTAAAGTAGAAGAAAGTGATTTTTTATTGAAAGAAAGTAAAGAAATTTTAAA
TTACATGATTAAAAATCAAGAATTGGACAAAATAACTATTGACAAATTGAAAAGTTTAAACATATCCGAAGAATACTTAA
AAGAATTAAATTCAATTTCTTTGAATAGTATAAACTTAGAGAACACAAAAGAAATAGAAGGAATAATAACAAATATCAGA
AAAAACTCATTAGAAGAGCAAATTAATAGCTTGTTGAGAGAACAACAAGAATTAGAAAATAATAATGATATGAAAGAGGT
AGATGGTAGAGTTATGGAAATTGCTTTAAAAATAGTTGAGATAAATAAAATTTTAAAGAGCTTGTAG

Upstream 100 bases:

>100_bases
AAAATAAAAATTTTTTGATTAATAAGAAGGATATCAATAAAAAAAGTCGAATTGTGTATATTGAGTGATTGACAAATTTA
GAATATAGTGTAGAATAGAA

Downstream 100 bases:

>100_bases
AGAAAGGAGGTTTGAGTGTGGAAAATAAATCGAATAAAAAAGAGTTAAAAAAGGTTACTGCTAAGACATTAATAGAAAAA
GGAAAAAAGCAAGGTTCTCT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MLTQKITPEGDNMNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEKTPSFYINTSKQIYKCFGCGEGGDV
INFVMKMENLDFMDAVKILANKCGIEINTNMNEETRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKI
IKKFGLGFSLDSWNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGRVLDDSLPKYL
NSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKNVVATLGTALTEQQGILIKRYADTAIISYDSDE
AGIKATLRAIDILTKLGINVKVLDLKDAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEA
SKIIKQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEVRQDGKQLVEETLIKIMLE
DKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITIDKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIR
KNSLEEQINSLLREQQELENNNDMKEVDGRVMEIALKIVEINKILKSL

Sequences:

>Translated_608_residues
MLTQKITPEGDNMNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEKTPSFYINTSKQIYKCFGCGEGGDV
INFVMKMENLDFMDAVKILANKCGIEINTNMNEETRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKI
IKKFGLGFSLDSWNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGRVLDDSLPKYL
NSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKNVVATLGTALTEQQGILIKRYADTAIISYDSDE
AGIKATLRAIDILTKLGINVKVLDLKDAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEA
SKIIKQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEVRQDGKQLVEETLIKIMLE
DKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITIDKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIR
KNSLEEQINSLLREQQELENNNDMKEVDGRVMEIALKIVEINKILKSL
>Mature_608_residues
MLTQKITPEGDNMNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEKTPSFYINTSKQIYKCFGCGEGGDV
INFVMKMENLDFMDAVKILANKCGIEINTNMNEETRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKI
IKKFGLGFSLDSWNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGRVLDDSLPKYL
NSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKNVVATLGTALTEQQGILIKRYADTAIISYDSDE
AGIKATLRAIDILTKLGINVKVLDLKDAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEA
SKIIKQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEVRQDGKQLVEETLIKIMLE
DKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITIDKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIR
KNSLEEQINSLLREQQELENNNDMKEVDGRVMEIALKIVEINKILKSL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=414, Percent_Identity=34.5410628019324, Blast_Score=269, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70104; Mature: 70104

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQKITPEGDNMNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEKTPSF
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
YINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEETRIKIE
EEECHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCEEEEH
KSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDSWNSLMNTL
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHH
ISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGRVLDDSLPKYL
HHCCCCHHHHHHHCCEECCCCCCHHHHHHHCCEEEEEECCCCCEEECCCCHHHHHHHHHH
NSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKNVVATLGTALTEQ
CCCCCEEEECCCCCEECHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLKDAKDPDEFVRKYGL
CCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCC
SDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKIIKQLTSPVEIDFYTKYL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
SNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEVRQDGKQLVEETLIKIMLE
CCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITIDKLKSLNISEEYLKELNSIS
CCHHCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCC
LNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQELENNNDMKEVDGRVMEIALKIVE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
INKILKSL
HHHHHHCC
>Mature Secondary Structure
MLTQKITPEGDNMNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEKTPSF
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
YINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEETRIKIE
EEECHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCEEEEH
KSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDSWNSLMNTL
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHH
ISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGRVLDDSLPKYL
HHCCCCHHHHHHHCCEECCCCCCHHHHHHHCCEEEEEECCCCCEEECCCCHHHHHHHHHH
NSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKNVVATLGTALTEQ
CCCCCEEEECCCCCEECHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLKDAKDPDEFVRKYGL
CCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCC
SDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKIIKQLTSPVEIDFYTKYL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
SNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEVRQDGKQLVEETLIKIMLE
CCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITIDKLKSLNISEEYLKELNSIS
CCHHCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCC
LNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQELENNNDMKEVDGRVMEIALKIVE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
INKILKSL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]