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The map label for this gene is rimO

Identifier: 126698925

GI number: 126698925

Start: 1537759

End: 1539093

Strand: Direct

Name: rimO

Synonym: CD1326

Alternate gene names: 126698925

Gene position: 1537759-1539093 (Clockwise)

Preceding gene: 126698924

Following gene: 126698926

Centisome position: 35.84

GC content: 28.54

Gene sequence:

>1335_bases
ATGTTAAAAATAGCATTGGAATCATTAGGATGCTCAAAAAATTTAGTAGATGCAGAAATAATGATGGGAATTTTAAATAA
CAAAGGCTATAAATTAATTGGAGATTTTGAAGAAGCTGATGTAATTATAGTAAATACATGTGGGTTTATAGAGTCAGCTA
AACAAGAATCTATAGATACGATAATAAACTTTGCAGAGTTAAAAAAAACTGGCAATTTAAAGTTGTTGATAGTAACAGGG
TGTCTAGCCCAAAGATATTCTGAAGAATTAAAAACTGAAATTCCTGAAATAGATGCTATAGTAGGAACAGGAAGCTATCA
AAATATAGATAAAATACTTAAAGAATTAAGTGAAATACATCAAATTGTAAGCCTTAATGATATAGAGTTTGTATTTAATG
AAGATTTACCAAGATATATATCTACACCTAGCTATATGGCCTATTTAAAAATTGGAGAAGGATGTTCCAATAATTGTACT
TATTGTATAATACCAAAACTAAGAGGTAAATATAGAAGTAGAAAATTTGAAGATATAATTAAAGAAGCTAAGAAATTAGC
TGAATCTGGAGTAAAAGAACTTGTGGTTATAGCACAGGATACGACAAAGTATGGTTTTGATTTATATGGAAAAGAAAGAT
TATCAGAACTTTTAGAAGAACTTGCAAAAATAGATGGATTTAAGTGGATAAGGGTTATGTATTCATATCCAGAATCTATA
ACAGAGGAATTGATACAAGTTATAAAGAAATATGATAATATATGTAGTTACTTTGATATGCCAATACAGCATGCAAGTAA
CAATATTTTAAAATTAATGAATAGAAAAACTACTAAAGAAGATATATTAAATAAAATCAATCTGATAAGAAGTAATATAC
CAGATGCTATACTTAGAACTACAATAATAGTAGGGTTTCCTGGTGAAACAGAAGATGACTTCAAACAACTTGTGGACTTT
GTTGAAGAGGTTAAATTTGATAGACTTGGTGCATTTGCATATTCAAGAGAAGAAGATACACCAGCAGATAGATTGCCAAA
CCATATAGATGAAGAAGTAAAAATACAAAGAAGAGATACTTTAATGATGATTCAACAAAAAATATCTGAAGAACTTAATG
ACAAAAAGATAGGAAAAACTTATGAGGTGTTAATAGAAGAACAGATAGAAGATAATGTATATACAGGAAGAACACAAGGT
GATGCGGAAGAAATTGACAGTATAGTGTATGTAAAAAGTGTAGACAATCTTGAAGTAGGAGAATTTGTAAGTGTTCAAAT
AAATGATGCAATGGAATACGATTTAATGGGAGATGTTTTATATGAACTTGCCTAA

Upstream 100 bases:

>100_bases
AGAAATATAAGTATAGTAAGATTATAAATTTTAATGATACACAAAAAGCTTTAGAAGATGTTATAGACTTATATGAGAGT
ATATTGGAAGGAGAAAGTGA

Downstream 100 bases:

>100_bases
CAAGTTAACTTTGTTTAGAATATTTTTAATACCAGTATTTGTTTTAATTATGTTGCTCAATGTACCAAATAAATTTTTAA
TAGCTTGTATTATTTTTATA

Product: radical SAM superfamily protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIVNTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTG
CLAQRYSEELKTEIPEIDAIVGTGSYQNIDKILKELSEIHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELLEELAKIDGFKWIRVMYSYPESI
TEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRKTTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDF
VEEVKFDRLGAFAYSREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDNVYTGRTQG
DAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA

Sequences:

>Translated_444_residues
MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIVNTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTG
CLAQRYSEELKTEIPEIDAIVGTGSYQNIDKILKELSEIHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELLEELAKIDGFKWIRVMYSYPESI
TEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRKTTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDF
VEEVKFDRLGAFAYSREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDNVYTGRTQG
DAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA
>Mature_444_residues
MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIVNTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTG
CLAQRYSEELKTEIPEIDAIVGTGSYQNIDKILKELSEIHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELLEELAKIDGFKWIRVMYSYPESI
TEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRKTTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDF
VEEVKFDRLGAFAYSREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDNVYTGRTQG
DAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=30.1052631578947, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=30.1052631578947, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI93277076, Length=412, Percent_Identity=28.1553398058252, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=39.2857142857143, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=29.3986636971047, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17553146, Length=454, Percent_Identity=24.8898678414097, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71996771, Length=294, Percent_Identity=27.5510204081633, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=28.1837160751566, Blast_Score=201, Evalue=9e-52,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=28.4463894967177, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CLOD6 (Q18BJ2)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087822.1
- ProteinModelPortal:   Q18BJ2
- SMR:   Q18BJ2
- STRING:   Q18BJ2
- GeneID:   4914613
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD1326
- NMPDR:   fig|1496.1.peg.3406
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK852941
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50756; Mature: 50756

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIVNTCGFIESAKQESIDT
CCEEEEHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHH
IINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVGTGSYQNIDKILKELSEIH
HHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHHHH
QIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCTYCIIPKLRGKYRSRKFEDII
HHHCCCCCCEEECCCCHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCHHHCHHHHHHH
KEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELLEELAKIDGFKWIRVMYSYPESI
HHHHHHHHCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
TEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRKTTKEDILNKINLIRSNIPDAILRT
HHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHH
TIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAYSREEDTPADRLPNHIDEEVKIQRRDT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHH
LMMIQQKISEELNDKKIGKTYEVLIEEQIEDNVYTGRTQGDAEEIDSIVYVKSVDNLEVG
HHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEECCCCCCCC
EFVSVQINDAMEYDLMGDVLYELA
CEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIVNTCGFIESAKQESIDT
CCEEEEHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHH
IINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVGTGSYQNIDKILKELSEIH
HHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHHHH
QIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCTYCIIPKLRGKYRSRKFEDII
HHHCCCCCCEEECCCCHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCHHHCHHHHHHH
KEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELLEELAKIDGFKWIRVMYSYPESI
HHHHHHHHCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
TEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRKTTKEDILNKINLIRSNIPDAILRT
HHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHH
TIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAYSREEDTPADRLPNHIDEEVKIQRRDT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHH
LMMIQQKISEELNDKKIGKTYEVLIEEQIEDNVYTGRTQGDAEEIDSIVYVKSVDNLEVG
HHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEECCCCCCCC
EFVSVQINDAMEYDLMGDVLYELA
CEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA