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The map label for this gene is trmD

Identifier: 126698853

GI number: 126698853

Start: 1458695

End: 1459390

Strand: Direct

Name: trmD

Synonym: CD1256

Alternate gene names: 126698853

Gene position: 1458695-1459390 (Clockwise)

Preceding gene: 126698852

Following gene: 126698854

Centisome position: 34.0

GC content: 29.45

Gene sequence:

>696_bases
ATGAGATTTCATATAATGACACTTTTTCCAGAAATATTTAATTCTTACATGGATGAAAGTATAATGAAGAGAGCAGTTGA
AAAAGGTATAATTGAGGTTCATATATATAATATAAGGGATTTTTCTAATAATAAACATAAAAAAGTAGATGATTATCCAT
TTGGAGGAGGAGCGGGGATGGTAATGACTCCTCAACCTATATATGACACATATAAGCATATAATAACAACTCATAATATA
AATAACCCAAGTGTAATTTATCTTACTCCAAAAGGTAAGGTATACAATCAATCTATGGCAAAGCAGATGTCTTTAAAAGA
AGATATAATACTACTATGTGGTCATTATGAAGGAATAGATGAGAGAATCATTGATTTAATAGTTACTGATGAAATTTCAA
TTGGAGATTATGTATTAACTGGGGGAGAACTCCCAGCACTTATAATGATAGATTCTATATCGAGATTAATACCAGGTGTA
TTAAACCAAGAAGAATCATTTGAAGAAGAATCATTTAAAGACAACTTATTGGAATATCCTCATTACACTAGACCTAGAGA
TTTTGAAGGATTAAAAGTACCAGAAGTGCTTTTATCAGGGAATCATAAGAAGATAGATGAGTGGAGAAGAGAAGAATCTA
TAAGGATAACCAAAGAAAGACGATTTGATTTATATAAAAAGTCTAATGAAAAATAA

Upstream 100 bases:

>100_bases
TTATGATACCAGCTATAAAAAAATTTGTACCTACAATAGATATTGATGAAAGAAAAATGATTATAGACCCTATTAAGGGA
ATGATAGATTAGGTGGTAAT

Downstream 100 bases:

>100_bases
AAATATAATGAAAAATTAATATATATGTATACAATAAAGTACCTATAAATGGAATTAAAAACATAAAAGTGCTTGTAATA
ACTATTGAAGTATAGTATAA

Product: tRNA (guanine-n(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNI
NNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERRFDLYKKSNEK

Sequences:

>Translated_231_residues
MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNI
NNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERRFDLYKKSNEK
>Mature_231_residues
MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNI
NNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERRFDLYKKSNEK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=46.4285714285714, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CLOD6 (Q18BC2)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087750.1
- ProteinModelPortal:   Q18BC2
- SMR:   Q18BC2
- STRING:   Q18BC2
- GeneID:   4913735
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD1256
- NMPDR:   fig|1496.1.peg.34
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   CLSK2534772
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26949; Mature: 26949

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGM
CEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCE
VMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGID
EECCCHHHHHHHHHEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHH
ERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGVLNQEESFEEESFKDNLLEYP
HHHEEEHEECCCCCCCEEEECCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
HYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERRFDLYKKSNEK
CCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGM
CEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCE
VMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGID
EECCCHHHHHHHHHEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHH
ERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGVLNQEESFEEESFKDNLLEYP
HHHEEEHEECCCCCCCEEEECCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
HYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERRFDLYKKSNEK
CCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA