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The map label for this gene is levE [H]

Identifier: 126698663

GI number: 126698663

Start: 1269131

End: 1269601

Strand: Direct

Name: levE [H]

Synonym: CD1076

Alternate gene names: 126698663

Gene position: 1269131-1269601 (Clockwise)

Preceding gene: 126698662

Following gene: 126698664

Centisome position: 29.58

GC content: 29.51

Gene sequence:

>471_bases
ATGATTAAATTAGTAAGAGTAGACCACAGACTTATACATGGACAAGTAGCATTTACATGGACAAAGTTTTTAAGTACAGA
CTGTATATTGATAGCAAGTGATGACTTATTAAAAGATGAATTGAGAATGGCAGGACTTAAAATGGCTAAACCATCTAATG
TTAAGTTAGTAATGAAAAGCATAGCAGATTCTATAAAAGCACTTAACTCAGGTGTTACTGATAAATACAACTTATTGATA
CTTTGTGAATCTGTAGAAGATGTTTATAGACTTGCTAAAGAGGTAAAAGCTATTAAATCTATAAACCTTGGTGGAACAAA
ATCAGATGATAATCGTGAAAATATATCTAAAGCAGTGCATGTATCAAAAGATGATATAAAGATGATTAAAGAGTTAGATT
CAGAAGGCGTAAATGTATTTGTACAATTAGTTCCTGATGATGATGCTACAAATGTAATGAAATTAATATAA

Upstream 100 bases:

>100_bases
GAAGTTACTTTAAAGATAATTACAAAGATTTTGGGGATGTTAGTGTAGAACACGTAAAAACATTCATGGATGAAATCAAA
AAAATTAGGGAGGAATTATA

Downstream 100 bases:

>100_bases
TTTTAGGGGGATGAAAATGGAATTTACACAAGTTATTTTGATAACTCTAATTGCATTCTTTGCATATATGCATAGTTTTG
TAGGTTCTACAATGCATAAT

Product: PTS system transporter subunit IIB

Products: NA

Alternate protein names: EIIB-Fru; PTS system fructose-specific EIIB component; p18 [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMAGLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLI
LCESVEDVYRLAKEVKAIKSINLGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI

Sequences:

>Translated_156_residues
MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMAGLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLI
LCESVEDVYRLAKEVKAIKSINLGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI
>Mature_156_residues
MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMAGLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLI
LCESVEDVYRLAKEVKAIKSINLGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator [H]

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI87082217, Length=155, Percent_Identity=29.0322580645161, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1788120, Length=156, Percent_Identity=32.0512820512821, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1789527, Length=152, Percent_Identity=25, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17373; Mature: 17373

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMAGLKMAKPSNVKLVMKS
CEEEEEECHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHH
IADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSINLGGTKSDDNRENISKAVH
HHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
VSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI
CCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCC
>Mature Secondary Structure
MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMAGLKMAKPSNVKLVMKS
CEEEEEECHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHH
IADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSINLGGTKSDDNRENISKAVH
HHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
VSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI
CCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377; 9551099 [H]