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The map label for this gene is speE

Identifier: 126698467

GI number: 126698467

Start: 1072076

End: 1072927

Strand: Direct

Name: speE

Synonym: CD0890

Alternate gene names: 126698467

Gene position: 1072076-1072927 (Clockwise)

Preceding gene: 126698466

Following gene: 126698468

Centisome position: 24.99

GC content: 29.34

Gene sequence:

>852_bases
ATGGAACTTTGGTATACAGAAGAATGGACAGAAAATGTAAGATTTTCTATAAAAGTAAATAAACATCTATTTGAAGGAAA
GTCTCAATTTCAAAGGATAGATGTGTTTGATAGTGATGAATTTGGAAAATTTCTAACAATAGATGGATTAATGATGGTAA
CATATAAAGATGAATTTATATACCATGAGATGATAACTCATGTTCCTATGGCTACAAATTTAAACATAAAGAAAGTATTA
GTCATAGGTGGAGGTGATGGAGGTACAGTAAGAGAATTAAGCCGTTATCCTCAAATAGAAAAGATTGACATGGTTGAAAT
AGATAAAATGGTTGTAGATGTATCTAAAGAGTATATGGATATATGCTCATGTAAATTAGATGATAAAAGAGTGAGTTTAT
ATTTTGAAGATGGTGTTAATTTTGTTAAGTGTGCACATGATAAATCATATGACCTGATAATAGTAGATTCAACTGACCCA
ATAGGACCAGGTGAAGGGCTTTTCTCAACTGATTTTTATAAGGATTGTTATAGAATACTGACTGATGATGGAATTTTAGT
AAATCAAAGTGAAAGTCCGTATTTTGACTTTAATGCTAAAGAGATGAAAAGAGCTAATAAAAAATTAAAACAGATATTTC
CAATATCAGAAGTTTATCAAGCACATATACCAACATATCCATCTGGTCACTGGTTGTTTGGATTTGCTTCAAAAAAACTT
AATCCAGTAAAAAATCAAGATAGAAATGGATGGGAGAAATTAAGTTTAAAAACTAAATATTATAATAGTGATATACATTT
AGGTTCGTTTATGTTACCTCAATATGTGAAGGAGATGCTAGATGAAGAATAA

Upstream 100 bases:

>100_bases
TAACTACAAAAATTAAAAAATATTCAAAAAAAGATTTAGGTAAGATTACTCATAAACCAGAAGCAGTATAAAACATACAA
GAGAAAGTGAGGATTATTAT

Downstream 100 bases:

>100_bases
TTTCTATCATATGAACACTTTTATGAGTATGGACAAAAATTATGAAGAATCTAATCTTATAGTATTTGGTGTTGGATTTG
ATGGAACTACTTCTAACAGA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKFLTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVL
VIGGGDGGTVRELSRYPQIEKIDMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEVYQAHIPTYPSGHWLFGFASKKL
NPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYVKEMLDEE

Sequences:

>Translated_283_residues
MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKFLTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVL
VIGGGDGGTVRELSRYPQIEKIDMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEVYQAHIPTYPSGHWLFGFASKKL
NPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYVKEMLDEE
>Mature_283_residues
MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKFLTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVL
VIGGGDGGTVRELSRYPQIEKIDMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEVYQAHIPTYPSGHWLFGFASKKL
NPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYVKEMLDEE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=289, Percent_Identity=35.6401384083045, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1786312, Length=272, Percent_Identity=39.3382352941176, Blast_Score=196, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI25154106, Length=273, Percent_Identity=38.4615384615385, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI212645616, Length=324, Percent_Identity=33.0246913580247, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323175, Length=274, Percent_Identity=36.8613138686131, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6325326, Length=269, Percent_Identity=35.6877323420074, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24645443, Length=283, Percent_Identity=35.6890459363958, Blast_Score=188, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21355941, Length=224, Percent_Identity=36.1607142857143, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_CLOD6 (Q18A85)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087364.1
- ProteinModelPortal:   Q18A85
- SMR:   Q18A85
- STRING:   Q18A85
- GeneID:   4914142
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0890
- NMPDR:   fig|1496.1.peg.1754
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   FVAQTES
- ProtClustDB:   PRK00811
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 32992; Mature: 32992

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 31-31 BINDING 86-86 BINDING 106-106 BINDING 156-156 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKFLTIDGLMMVTYKDEFI
CCCEECCCCCCCEEEEEEECCHHHCCHHHHEEECCCCCCCCCCEEEECCEEEEEEECHHH
YHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKIDMVEIDKMVVDVSKEYMD
HHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDPIGPGEGLFSTDFYKDCYRIL
HHCCCCCCCEEEEEEECCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
TDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEVYQAHIPTYPSGHWLFGFASKKL
CCCCEEEECCCCCEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCC
NPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYVKEMLDEE
CCCCCCCCCCHHEEEEEEEEECCCEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKFLTIDGLMMVTYKDEFI
CCCEECCCCCCCEEEEEEECCHHHCCHHHHEEECCCCCCCCCCEEEECCEEEEEEECHHH
YHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKIDMVEIDKMVVDVSKEYMD
HHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDPIGPGEGLFSTDFYKDCYRIL
HHCCCCCCCEEEEEEECCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
TDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEVYQAHIPTYPSGHWLFGFASKKL
CCCCEEEECCCCCEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCC
NPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYVKEMLDEE
CCCCCCCCCCHHEEEEEEEEECCCEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA