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The map label for this gene is oppA [H]

Identifier: 126698435

GI number: 126698435

Start: 1030462

End: 1032030

Strand: Direct

Name: oppA [H]

Synonym: CD0855

Alternate gene names: 126698435

Gene position: 1030462-1032030 (Clockwise)

Preceding gene: 126698434

Following gene: 126698436

Centisome position: 24.02

GC content: 30.72

Gene sequence:

>1569_bases
ATGAAATTAAAGAAGTTAAAAGTTTTAAGTTTAGTAATGATACTTAGTCTAATGGCAGGTTGTTCTAGTGGAGGAGACAA
AGATAAGAAGGCAGATACGCCTAAAGATGGAAAGGTACTTGTTTATGGAAGTAATGACTATACAAGTATAAATCCTGCAC
TATACGAACATGGAGAAATAAATTCGTTAATATTTAATGGATTAACAGCTCATGATGAAAATAATAAGGTGGTTCCTTGT
CTAGCAAAGGACTGGAAATTTGATGAGGCAACAAATACATATACTTTTAATTTAAGAGATGATGTTAAGTGGCATGATGG
AGAAAAATTTACAGCAAATGATGTTAAGTTTACAATAGAAACTATAATGAATCCAGATAATGCTTCTGAGATAGCATCAA
ATTATGAAGATATAACAAAAATTGATGTGGTTAATGATAATACTATAAAAATTACTTTAAAAGCTCCAAATACAGCAATG
CTTGATTATTTAACAGTAGGAGTATTACCAAAACATGCTTTGGAAGGTAAGGATATAGCTACTGATGAATTTAATCAAAA
ACCAATAGGTACTGGACCATTTAAACTTGAAAAATGGGACAAAGGTCAAAGTATAACACTTGTTAAGAATAGTGATTATT
TTGTAAAAGAACCAGGATTAGATAAAGTAGTCTTTAAGATTGTTCCAGATGATAAGGCTAAGGCAATGCAATTAAAATCA
GGAGAATTAGACCTAGCTCAAATAACTCCTAAAGATATGTCTAACTTTGAAAAAGACGAAAAGAATTTTAAAGTAAATAT
AATGAAAACAGCCGACTATAGAGGTATATTATACAATTTTAATTCTAAATTCTTTAAAGATAAAAAAGCTAAAGGATTAC
CAAATGCTTTAAGCTATGCAATAGATAGAAAAGCAATAGTTGACAGTGTATTATTAGGACATGGTGTACCAGCATATTCA
CCTTTACAAATGGGACCATATAATAATCCTGATATAGAAAAGTTTGAATATAATCCAGAAAAAGCTAAACAAGAAATTGA
AAAGCTAGGTTGGAAATTAGGTTCTGATGGAATCTATGAAAAAGAAGGTACTAAGTTAGCTTTCGAGATAACAGCAGGTG
AGAGTGACCAAGTTAGAGTTGATATGGCTAAAATATGTGCACAACAGTTAAAAGAAATTGGTGTTGATGCTAAAGCTGTT
GTTGTAACTGAAACAGATTGGGCTAATCAAGATGCTCATTTAATAGGTTGGGGAAGTCCATTTGACCCAGATGACCATAC
ATATAAAGTATTTGGAACAGATAAAGGTGCAAATTACAGTGCTTACTCTAATCCTACTATAGATAAAATACTTCAAAAAG
CAAGAGAAACAGAAGATAAAGATGAAAAATTAAAGTTATATAAACAATTCCAAGTAGAAATGACTAAGGATATGCCTTAT
ACATTTATTGCTTATATAGATGCAATATATGTTGGAAAACCAAACATAAAAGGATTAACACCTGATACAGTTTTAGGTCA
TCATGGTGTAGGTATATTCTGGAACATAGCTGACTGGACAATTGAATAA

Upstream 100 bases:

>100_bases
GTATTAAATAAGCAAATACTGAGGTATTTGAAATATCTATTTAAATAAATGGGGTCAAAGCTTTGAAAAATATTTTATAA
AAAGCAAAAGGGGGAGCATC

Downstream 100 bases:

>100_bases
TACTGAAATTAATTAGAAACCTTAGGGATAAAAATATATCCCTAAGGTTTGAATAGCATAGGGGTTGATGGTTTTTGGAA
CATTTATTGGAGGTTAATAA

Product: oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGSNDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPC
LAKDWKFDEATNTYTFNLRDDVKWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEPGLDKVVFKIVPDDKAKAMQLKS
GELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGILYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYS
PLQMGPYNNPDIEKFEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKEIGVDAKAV
VVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKILQKARETEDKDEKLKLYKQFQVEMTKDMPY
TFIAYIDAIYVGKPNIKGLTPDTVLGHHGVGIFWNIADWTIE

Sequences:

>Translated_522_residues
MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGSNDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPC
LAKDWKFDEATNTYTFNLRDDVKWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEPGLDKVVFKIVPDDKAKAMQLKS
GELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGILYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYS
PLQMGPYNNPDIEKFEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKEIGVDAKAV
VVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKILQKARETEDKDEKLKLYKQFQVEMTKDMPY
TFIAYIDAIYVGKPNIKGLTPDTVLGHHGVGIFWNIADWTIE
>Mature_522_residues
MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGSNDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPC
LAKDWKFDEATNTYTFNLRDDVKWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEPGLDKVVFKIVPDDKAKAMQLKS
GELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGILYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYS
PLQMGPYNNPDIEKFEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKEIGVDAKAV
VVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKILQKARETEDKDEKLKLYKQFQVEMTKDMPY
TFIAYIDAIYVGKPNIKGLTPDTVLGHHGVGIFWNIADWTIE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=560, Percent_Identity=28.2142857142857, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1789887, Length=478, Percent_Identity=26.7782426778243, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1787052, Length=530, Percent_Identity=25.2830188679245, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787762, Length=422, Percent_Identity=27.9620853080569, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1787551, Length=533, Percent_Identity=20.4502814258912, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789397, Length=531, Percent_Identity=22.2222222222222, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787495, Length=542, Percent_Identity=22.5092250922509, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081878, Length=423, Percent_Identity=23.1678486997636, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87082063, Length=252, Percent_Identity=23.015873015873, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58515; Mature: 58515

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGSNDYTSINPALYEHGEI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCC
NSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDVKWHDGEKFTANDVKFTIE
CEEEECCEEEECCCCCEEEEEECCCCCCCCCCEEEEEECCCCEECCCCEEECCCEEEEEE
TIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAMLDYLTVGVLPKHALEGKDIA
ECCCCCCHHHHHHCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHHCCCCHHHCCCCCCC
TDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEPGLDKVVFKIVPDDKAKAMQLKS
CCCCCCCCCCCCCEEEEECCCCCEEEEEECCCEEEECCCCCEEEEEECCCCCCCEEEECC
GELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGILYNFNSKFFKDKKAKGLPNALSYA
CCEEEEEECCHHHHHCCCCCCCEEEEEEEECCCCEEEECCCCHHHHCCCCCCCCHHHHHH
IDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEKFEYNPEKAKQEIEKLGWKLGSDGIYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCEE
KEGTKLAFEITAGESDQVRVDMAKICAQQLKEIGVDAKAVVVTETDWANQDAHLIGWGSP
CCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCC
FDPDDHTYKVFGTDKGANYSAYSNPTIDKILQKARETEDKDEKLKLYKQFQVEMTKDMPY
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCE
TFIAYIDAIYVGKPNIKGLTPDTVLGHHGVGIFWNIADWTIE
EEEEEHHEEEECCCCCCCCCCCCEECCCCCEEEEEECCEEEC
>Mature Secondary Structure
MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGSNDYTSINPALYEHGEI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCC
NSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDVKWHDGEKFTANDVKFTIE
CEEEECCEEEECCCCCEEEEEECCCCCCCCCCEEEEEECCCCEECCCCEEECCCEEEEEE
TIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAMLDYLTVGVLPKHALEGKDIA
ECCCCCCHHHHHHCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHHCCCCHHHCCCCCCC
TDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEPGLDKVVFKIVPDDKAKAMQLKS
CCCCCCCCCCCCCEEEEECCCCCEEEEEECCCEEEECCCCCEEEEEECCCCCCCEEEECC
GELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGILYNFNSKFFKDKKAKGLPNALSYA
CCEEEEEECCHHHHHCCCCCCCEEEEEEEECCCCEEEECCCCHHHHCCCCCCCCHHHHHH
IDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEKFEYNPEKAKQEIEKLGWKLGSDGIYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCEE
KEGTKLAFEITAGESDQVRVDMAKICAQQLKEIGVDAKAVVVTETDWANQDAHLIGWGSP
CCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCC
FDPDDHTYKVFGTDKGANYSAYSNPTIDKILQKARETEDKDEKLKLYKQFQVEMTKDMPY
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCE
TFIAYIDAIYVGKPNIKGLTPDTVLGHHGVGIFWNIADWTIE
EEEEEHHEEEECCCCCCCCCCCCEECCCCCEEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]