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The map label for this gene is gcvH

Identifier: 126698309

GI number: 126698309

Start: 892293

End: 892670

Strand: Direct

Name: gcvH

Synonym: CD0729

Alternate gene names: 126698309

Gene position: 892293-892670 (Clockwise)

Preceding gene: 126698308

Following gene: 126698310

Centisome position: 20.8

GC content: 31.48

Gene sequence:

>378_bases
ATGAAATTATTACCAGAATTAAAATACTCTAAAGATCATGAGTGGGTAAAAGTAATTGATGGAGATGTTGTATATATAGG
TATAACAGACTATGCTCAAGATCAATTAGGAGAAATATTGTTTGTTGAAACACCAGAAGTAGAAGATACTGTAACTAAGG
GAGTAGATTTTGGAGTAGTTGAGTCTTCTAAAGTAGCTTCTGATTTAATATCTCCTGTTAATGGTGAGGTATTGGAAGTT
AATGAAAAATTAGAAGATGAGCCAGAATGTATAAATGAAGACCCATATGAAAACTGGATATTAAAAGTAAAACTAGCTGA
TGTGGCTGAACTTGATACGTTATTAAGTGATAAAGAATACGAGGCTGGATTAGAATAA

Upstream 100 bases:

>100_bases
AAAGTGTAATGTAATTGATGGAAAATTTAAAATAATATGATATAATATTAATATGATTTAAGTAATTTAATCAATAATAT
AATATTGGGGGTCTATCGAA

Downstream 100 bases:

>100_bases
TTAAAGATTAAGATATTATTATAATTGGGGAAAGTATAGTAAAATTTGAGTGCACGCAAATTTAGCTATACTTTCCTTTT
ATCATATATAGAACAAATTG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVETPEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEV
NEKLEDEPECINEDPYENWILKVKLADVAELDTLLSDKEYEAGLE

Sequences:

>Translated_125_residues
MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVETPEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEV
NEKLEDEPECINEDPYENWILKVKLADVAELDTLLSDKEYEAGLE
>Mature_125_residues
MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVETPEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEV
NEKLEDEPECINEDPYENWILKVKLADVAELDTLLSDKEYEAGLE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=40.8333333333333, Blast_Score=100, Evalue=6e-22,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=40, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=48.0314960629921, Blast_Score=116, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=44.2477876106195, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=44.2477876106195, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=38.5245901639344, Blast_Score=96, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=39.0243902439024, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_CLOD6 (Q189S4)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087206.1
- ProteinModelPortal:   Q189S4
- SMR:   Q189S4
- STRING:   Q189S4
- GeneID:   4914193
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0729
- NMPDR:   fig|1496.1.peg.1921
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   THEWVRP
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14047; Mature: 14047

Theoretical pI: Translated: 3.76; Mature: 3.76

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVETPEVEDTVTKGVDFGVV
CCCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHCCCCEEEEECCCCHHHHHCCCCCCCC
ESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILKVKLADVAELDTLLSDKEY
CCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCC
EAGLE
CCCCC
>Mature Secondary Structure
MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVETPEVEDTVTKGVDFGVV
CCCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHCCCCEEEEECCCCHHHHHCCCCCCCC
ESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILKVKLADVAELDTLLSDKEY
CCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCC
EAGLE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA