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The map label for this gene is folD

Identifier: 126698300

GI number: 126698300

Start: 882050

End: 882922

Strand: Direct

Name: folD

Synonym: CD0720

Alternate gene names: 126698300

Gene position: 882050-882922 (Clockwise)

Preceding gene: 126698299

Following gene: 126698301

Centisome position: 20.56

GC content: 33.33

Gene sequence:

>873_bases
ATGGAAGGAATGTCAACTAAAGGACAAATTATAAAAGGAAAACCTGTAGCTGATAAGATTAGTGAAGAATTAATAAAAGA
AGTCGATTTACTAGTTAAAGAAGGTATAAATCCTAAACTAACAATAGTTAGAGTTGGGGCAAGAAGTGATGATTTATCCT
ATGAAAGAGGAGCACTAAAGAGATGTCAAAACATAGGTATAACAACAGAAGTGTTAGAGTTAGCTGAGGATATAACACAA
GAGGAATATATTGATGTATTAAAGAGAGTTAATGATGACAAAAATGTTAATGGTATATTATGTTTTAGACCTCTTCCAAA
ACACTTAAATGAAGAAGTTATAAAATATGTTATTGCACCGGAAAAAGACGTAGATTGTTTTAGTCCAATAAACTCTGCTA
AGGTAATGGAAGGGGATAAAAGTGGATTTCCACCATGTACACCTACAGCAGTAGTTGAGATATTAAAACACTATAATGTT
GATTTAAAAGGAAGTAAGGTAACAGTTCTCGGAAGATCAATGGTAGTTGGAAAGCCAGTATCAATGCTTTTATTAAGTGA
ACACGCAACTGTTACAATATGTCATTCAAAAACTAAAAATCTATCAGGTGTAGCAGCAGAGGCAGACGTGCTAATAGCAG
CTATTGGTAGAGCTAAGATGGTTGATGAAAGTTTTGTTAAAGATGGAGCGGTAGTTATAGACGTTGGTATAAATGTTGAT
GAAGAAGGAAATTTATGTGGAGATGTTGATACAAATGCAGTTTTAGACAAAGTATCAATGATAACTCCAGTTCCAGCGGG
GGTTGGTTCAGTTACAACATCTATACTTGCAAAACATGTAGTTAAGGCTTGTAAATTACAAAACAATAAATAG

Upstream 100 bases:

>100_bases
GGAAAATAATTTTTAAATGACGAAAGTAGATACTATGTTATTAATATGTGGTATATAGAATAAATTAATTTAAGTTTGCT
TGTAGGGGAGTTGTGAATGT

Downstream 100 bases:

>100_bases
ATATATATATGTGGAAGGTTTAGGGGGGAAGTAGATATGATAATTTCTGAAAATAAACCATTAGAAGAAGTGCTAGGGTA
CTTAAAAGATTTTGACAAGC

Product: FolD bifunctional protein

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVRVGARSDDLSYERGALKRCQNIGITTEVLELAEDITQ
EEYIDVLKRVNDDKNVNGILCFRPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRAKMVDESFVKDGAVVIDVGINVD
EEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSILAKHVVKACKLQNNK

Sequences:

>Translated_290_residues
MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVRVGARSDDLSYERGALKRCQNIGITTEVLELAEDITQ
EEYIDVLKRVNDDKNVNGILCFRPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRAKMVDESFVKDGAVVIDVGINVD
EEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSILAKHVVKACKLQNNK
>Mature_290_residues
MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVRVGARSDDLSYERGALKRCQNIGITTEVLELAEDITQ
EEYIDVLKRVNDDKNVNGILCFRPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRAKMVDESFVKDGAVVIDVGINVD
EEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSILAKHVVKACKLQNNK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=37.5, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI222418558, Length=299, Percent_Identity=39.4648829431438, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=36.3013698630137, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=35.3790613718412, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17568735, Length=255, Percent_Identity=38.8235294117647, Blast_Score=163, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6319558, Length=292, Percent_Identity=38.6986301369863, Blast_Score=197, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=36.7892976588629, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6322933, Length=328, Percent_Identity=23.780487804878, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=40.2097902097902, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=40.2097902097902, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=40.2097902097902, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=40.2097902097902, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17136816, Length=303, Percent_Identity=35.3135313531353, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=35.472972972973, Blast_Score=158, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CLOD6 (Q189R8)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087197.1
- ProteinModelPortal:   Q189R8
- SMR:   Q189R8
- STRING:   Q189R8
- GeneID:   4914184
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0720
- NMPDR:   fig|1496.1.peg.1930
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   GFPPCTP
- ProtClustDB:   CLSK2534597
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31231; Mature: 31231

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVRVGARSDDLSYERGALK
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHH
RCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCFRPLPKHLNEEVIKYVIAP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC
EKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNVDLKGSKVTVLGRSMVVGKPV
CCCCHHHCCCCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCEECCCCH
SMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRAKMVDESFVKDGAVVIDVGINVD
HEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCC
EEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSILAKHVVKACKLQNNK
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVRVGARSDDLSYERGALK
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHH
RCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCFRPLPKHLNEEVIKYVIAP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC
EKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNVDLKGSKVTVLGRSMVVGKPV
CCCCHHHCCCCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCEECCCCH
SMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRAKMVDESFVKDGAVVIDVGINVD
HEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCC
EEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSILAKHVVKACKLQNNK
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA