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The map label for this gene is cspR [H]

Identifier: 126698145

GI number: 126698145

Start: 675554

End: 676024

Strand: Direct

Name: cspR [H]

Synonym: CD0566

Alternate gene names: 126698145

Gene position: 675554-676024 (Clockwise)

Preceding gene: 126698144

Following gene: 126698146

Centisome position: 15.75

GC content: 31.42

Gene sequence:

>471_bases
ATGTCATTAAATATAGTATTAGTAGAACCAGAAATACCTCAAAATACAGGAAATATAATAAGAACCTGTGCAGCAACAGG
TTCAACTTTACATTTAGTAAAACCACTGGGTTTTTCATTGGATGATAAACACCTAAAAAGATGTGGGCTTGACTATTGGG
ATATAGCTGATATACAATATTACGATAGCTTTGAAGAATTACAAGAAAAGTATCCAAATAGTAAATACTTTTTTTCTACA
ACAAAGGCTAAACAGTCACATTCAGATGTGAAATATGAAGAAAATTGTTTTATAGTATTTGGGAAAGAAACAAAGGGATT
ACCAGAACCTTTGTTGAAAGCAAATATGGATACTTGTATTAGAGTGCCTATGTTGGATGTTGAAAAAGCTAGGTCATTAA
ATTTATCAAATTCAGTTGCTATTGTAGTGTACGAGGCTTTAAGACAAATAGGATACCCAAATATGAGGTAG

Upstream 100 bases:

>100_bases
ATTATTATTAACATTATTTTATATAATAGTATAATATTATTGTTATTCTGTTTATAAAATAAAATTTGATAAATAAAAGC
GATACTTAGGGAGGATTAAA

Downstream 100 bases:

>100_bases
TTTCAACAAGCCTAAATGAGAAAGGAGCATACTTAATGAAAAATCTAAAGATAATCTGTGACAGTTTATCGGACGTGCCA
AAAGATTTGTTAGATAAATA

Product: RNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKHLKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFST
TKAKQSHSDVKYEENCFIVFGKETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR

Sequences:

>Translated_156_residues
MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKHLKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFST
TKAKQSHSDVKYEENCFIVFGKETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR
>Mature_155_residues
SLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKHLKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTT
KAKQSHSDVKYEENCFIVFGKETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=49.0196078431373, Blast_Score=154, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17786; Mature: 17654

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKHLKRCGLDYWDIADIQY
CCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCHH
YDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGKETKGLPEPLLKANMDTCI
HCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEE
RVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR
ECCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKHLKRCGLDYWDIADIQY
CEEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCHH
YDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGKETKGLPEPLLKANMDTCI
HCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEE
RVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR
ECCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]