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The map label for this gene is nth [H]

Identifier: 126698144

GI number: 126698144

Start: 674755

End: 675360

Strand: Direct

Name: nth [H]

Synonym: CD0565

Alternate gene names: 126698144

Gene position: 674755-675360 (Clockwise)

Preceding gene: 126698143

Following gene: 126698145

Centisome position: 15.73

GC content: 32.34

Gene sequence:

>606_bases
TTGGAAAAATTGTATCCAGATGCTAAATGTGAGTTAAATTATGGGACAGCCTTTGAACTTTTAATAGCAACTATTTTATC
AGCACAATGTACTGATGTAAGAGTGAATAAAGTTACTTCAGAGTTGTTTAAAAAGTACAACACAGCTAGAGACTTTGCTA
ATTTGAGCATTGAAGAAATTAGTAAAGAGATAAAGAGTTGTGGTCTTTACAAAAGCAAGTCTCAAAAAATAAAGGATACA
TCTGAACAGCTATGTGAATTATATGATGGTGAAGTACCTGATAGCTTAGAAAAGCTTATAAAATTACCTGGTGTTGGAAG
AAAAACAGCAGGGGTTGTACTTAGTAACGCTTTTAATCATCCAGCAATAGCTGTAGATACTCATGTATTTAGAGTTAGCA
ATAGAATAGGAATAGTGGACGAGCCAAATCCTCAAAAAACAGAATTTGCACTTATGGAAGCTATACCAAAAGAAAGATGG
TCACACTCACATCATGTATTAATTTTTCATGGAAGAAGAATGTGTAAAGCTAGAAATCCAGAATGTGCAAGTTGCCCAAT
AAAAGAAGATTGTAATTATTATAAAGAACTTAATGAAACGAAATAA

Upstream 100 bases:

>100_bases
TAGTAAGGTTACACAAAAACAAAGCAAAGAAGTTAAAAGTAAGTCTAAAATTAAAAATGAGTCAGCAAATGAAAAAGACG
TAAATAAGATTTTAGACGAG

Downstream 100 bases:

>100_bases
CTTTTAGAAAGTAAGTTGTACAAGAGGGATTTTCTGATATAATAATACTTGTGAAAATGTTAGATAAAAAACTATTATAT
ACATGAAATAAAAATTATTA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MEKLYPDAKCELNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDT
SEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQKTEFALMEAIPKERW
SHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK

Sequences:

>Translated_201_residues
MEKLYPDAKCELNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDT
SEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQKTEFALMEAIPKERW
SHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK
>Mature_201_residues
MEKLYPDAKCELNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDT
SEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQKTEFALMEAIPKERW
SHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=171, Percent_Identity=31.5789473684211, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI190358497, Length=189, Percent_Identity=24.8677248677249, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI6912520, Length=189, Percent_Identity=24.8677248677249, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI115298654, Length=189, Percent_Identity=24.8677248677249, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI115298652, Length=189, Percent_Identity=24.8677248677249, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI115298650, Length=189, Percent_Identity=24.8677248677249, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI115298648, Length=189, Percent_Identity=24.8677248677249, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787920, Length=193, Percent_Identity=46.6321243523316, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1789331, Length=184, Percent_Identity=25, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554540, Length=176, Percent_Identity=33.5227272727273, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=192, Percent_Identity=27.6041666666667, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=32.2033898305085, Blast_Score=106, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 22784; Mature: 22784

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLYPDAKCELNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEI
CCCCCCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKEIKSCGLYKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNH
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCC
PAIAVDTHVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNP
CEEEEHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCC
ECASCPIKEDCNYYKELNETK
CCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKLYPDAKCELNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEI
CCCCCCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKEIKSCGLYKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNH
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCC
PAIAVDTHVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNP
CEEEEHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCC
ECASCPIKEDCNYYKELNETK
CCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]