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The map label for this gene is nfo

Identifier: 126698139

GI number: 126698139

Start: 667037

End: 667873

Strand: Direct

Name: nfo

Synonym: CD0560

Alternate gene names: 126698139

Gene position: 667037-667873 (Clockwise)

Preceding gene: 126698138

Following gene: 126698140

Centisome position: 15.55

GC content: 31.78

Gene sequence:

>837_bases
ATGATTAAATTAGGAACACATCTTTCAATAGCAAAAGGATTTGCTAATGCGGCTGAGACAGCTGTATATATTGGTGCAAA
TACATTTCAATTTTTTAGTAGAAATCCAAGAGGAGGAAATGCTAAAGAATTTGATGAAAAAGATATAATTAAATTTCAAG
AAATTAGAAAAGAAAATAATTTTGCACCACTGCTTGCACATGCACCATATACTATGAATCTGGGTGGTACAAAGGATGAT
GTATACGAATTTGCAACAAGGGTGATAGATGAGGATATAAGAAGGATGGACTCATTAGGAATTGAATACATGTGTTTTCA
TCCTGGAAGTCATGTAGGTGGTGGTGTAGATTTTGGCATAGATAGAATAGTAAAAGGATTGAACAATGCCATAAAGGGTG
ATGAAAATATAACTATACTTCTTGAAACTATGTCGGGAAAGGGAACTGAAATAGGTTTTAAATTTGAGCAATTAAAAAGT
ATAATAGACAAAATTGAGCATAAAGAAAAAATAGGTGTGTGTCTTGATACATGTCATATTTTTTCAGCTGGTTATGATAT
AGTGAATGATTTAGATGGGGTTTTGGAAGAATTTGATAAGGTTATAGGCATAGAAAGATTGAAAACTGTCCATCTAAATG
ATAGTATGATGCCATTTGGAGACAAAAAAGACAGACATGCTCCTATAGGGGAAGGAAAGATAGGATTAAAAGCATTAATT
GATTTTATGGAACATCCTAGTCTAAAACATTTACCATTTTTCTTAGAAACTCCTTTTGATGATGAAGGTCACAAGAGAGA
GCTTAAAATGATAAAAGAAATACTATCTAGTAAATAA

Upstream 100 bases:

>100_bases
AGGAATCTCCATCTTCATATGTTGGCTAAATGTAGTTAAGGTGGAGTAGTTCAAAATTAAAATTGAGTACAAGTAGTAGT
ACAAATAAAAGGAGATTAAT

Downstream 100 bases:

>100_bases
ACCTTTATTAAAAATATGTGAAATACTCCGTCCTCAACATAATTAGGGTGGAGATATAATCATGAAATGTGATAATTTAA
ATTAATGGATAGGAATAACA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEFDEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDD
VYEFATRVIDEDIRRMDSLGIEYMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMPFGDKKDRHAPIGEGKIGLKALI
DFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILSSK

Sequences:

>Translated_278_residues
MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEFDEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDD
VYEFATRVIDEDIRRMDSLGIEYMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMPFGDKKDRHAPIGEGKIGLKALI
DFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILSSK
>Mature_278_residues
MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEFDEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDD
VYEFATRVIDEDIRRMDSLGIEYMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMPFGDKKDRHAPIGEGKIGLKALI
DFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILSSK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=262, Percent_Identity=34.7328244274809, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17531193, Length=260, Percent_Identity=38.4615384615385, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322735, Length=282, Percent_Identity=36.1702127659575, Blast_Score=181, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_CLOD6 (Q189A4)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087036.1
- ProteinModelPortal:   Q189A4
- SMR:   Q189A4
- STRING:   Q189A4
- GeneID:   4916198
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0560
- NMPDR:   fig|1496.1.peg.1587
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   CLSK2786227
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31179; Mature: 31179

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEFDEKDIIKFQEIRKENN
CEECCCCHHHHHHHHHHHHCEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
FAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIEYMCFHPGSHVGGGVDFGI
CCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHH
DRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKSIIDKIEHKEKIGVCLDTCHI
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMPFGDKKDRHAPIGEGKIGLKALI
HHHCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
DFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILSSK
HHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEFDEKDIIKFQEIRKENN
CEECCCCHHHHHHHHHHHHCEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
FAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIEYMCFHPGSHVGGGVDFGI
CCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHH
DRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKSIIDKIEHKEKIGVCLDTCHI
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMPFGDKKDRHAPIGEGKIGLKALI
HHHCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
DFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILSSK
HHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA