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The map label for this gene is manZ [H]

Identifier: 126698071

GI number: 126698071

Start: 583393

End: 584232

Strand: Direct

Name: manZ [H]

Synonym: CD0494

Alternate gene names: 126698071

Gene position: 583393-584232 (Clockwise)

Preceding gene: 126698070

Following gene: 126698072

Centisome position: 13.6

GC content: 32.26

Gene sequence:

>840_bases
ATGTCAGATATAAAAAATGAAGTATCTAAGAAAACGTTAAGCAAGAAAGATGTAATAAAATCATGGTTAAGATGGTTTTT
CTTTGCACAATCTAATTACAATTATGAAAGATTGCAATCAACAGCTTTTTCACATTCAATGTTGCCAGTACTTAAAAAAT
TATATCCAGATAAGGAAGAGTTAAAACAAGAAGTGGAGACTCATCTAGCATTTTTTAACACAGAACCTATATGTGGATGT
GTTATACATGGAATAACAATAGCTATGGAAGAAGAAAAAGCTAATGGAGCAGATGAAATAAGTGGAGATGGTATGAATGC
TATCAAAACAGGCTTGATGGGTCCACTAGCGGGAATTGGCGATACTTTAACTCAAGGAGTTATAACTCCTATAGTGTTGG
CTGTTTGTATTGGTTTAACTGAGGGAGGAGCATCAATTGCAGGACCTATATTATTTGTAATAGCTCAATATATAATAATG
ACTTCAATTTCTTTTGGAATGTGGACAAATGGATATAAGTATGGGAAAAAAGCTGTTGAAAGTATACTTCAAGGTGGAAT
AGTAAATAAGGTTATAGAAGGGGCTTCTATACTAGGTACCTTAGTCATGGGGGGACTTGTAGGTAGGTTTATTAATTTAT
CTACACCAATAAGTTATACTAGTGGTGATTTTAAATTTAGTCTTCAAACGGATTTACTTGATAAGATATTTCCTGGGTTA
ATTCCTTTGGTACTTACTTTATTAGTACTCTTTGCTTTAAAAAAAGGTCTTTCTCCAATAAAAATAATGTGTATACTGAT
AGTAGTGGGAGCTATTACAGGTATTTTAGGACTATTTTAA

Upstream 100 bases:

>100_bases
TAATATCTGTTATTGGAGTAATACTTGCTCTTACAATTACTTCAATTAAGTACGGAAAAGATAGTGCTACATCTTAAATA
CATGTGAGGGGGAAAATATT

Downstream 100 bases:

>100_bases
ATAAATATATATTGGTGTAAGTTAGGATTCTATATCTATTGATTATATTTGTAGGATGTTGGAAAATTTATATTAAAAAG
TTTGGCAATTAGGGTAATTT

Product: PTS system transporter subunit IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFSHSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGC
VIHGITIAMEEEKANGADEISGDGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPISYTSGDFKFSLQTDLLDKIFPGL
IPLVLTLLVLFALKKGLSPIKIMCILIVVGAITGILGLF

Sequences:

>Translated_279_residues
MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFSHSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGC
VIHGITIAMEEEKANGADEISGDGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPISYTSGDFKFSLQTDLLDKIFPGL
IPLVLTLLVLFALKKGLSPIKIMCILIVVGAITGILGLF
>Mature_278_residues
SDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFSHSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCV
IHGITIAMEEEKANGADEISGDGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIMT
SISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPISYTSGDFKFSLQTDLLDKIFPGLI
PLVLTLLVLFALKKGLSPIKIMCILIVVGAITGILGLF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=287, Percent_Identity=37.2822299651568, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789529, Length=277, Percent_Identity=31.7689530685921, Blast_Score=140, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 30146; Mature: 30015

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFSHSMLPVLKKLYPDKEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
LKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISGDGMNAIKTGLMGPLAGIG
HHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIMTSISFGMWTNGYKYGKKAVE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPISYTSGDFKFSLQTDLLDKIFPGL
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHH
IPLVLTLLVLFALKKGLSPIKIMCILIVVGAITGILGLF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]