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The map label for this gene is hadI [H]

Identifier: 126697965

GI number: 126697965

Start: 468262

End: 469071

Strand: Direct

Name: hadI [H]

Synonym: CD0396

Alternate gene names: 126697965

Gene position: 468262-469071 (Clockwise)

Preceding gene: 126697964

Following gene: 126697966

Centisome position: 10.91

GC content: 33.58

Gene sequence:

>810_bases
ATGCTAAAGATGTACACAATGGGATTAGATATAGGTTCAACTGCATCAAAGGGAGTAATCTTAAAGAATGGGGAAGATAT
TGTAGCTTCTGAAACAATATCCTCTGGTACTGGGACTACTGGACCATCAAGAGTTTTAGAAAAATTATATGGCAAGACAG
GTCTTGCAAGAGAAGATATTAAAAAAGTTGTAGTTACAGGATATGGAAGAATGAACTATTCAGATGCTGATAAGCAAATA
AGTGAATTAAGCTGTCATGCTAGAGGGGTAAATTTCATAATTCCAGAGACAAGAACCATTATTGACATAGGCGGTCAAGA
TGCAAAGGTATTAAAATTAGATAATAATGGAAGACTATTAAACTTTCTTATGAATGACAAATGTGCTGCAGGTACAGGAA
GATTTTTAGATGTAATGGCAAAAATAATAGAGGTTGATGTATCTGAACTCGGAAGTATATCTATGAATTCTCAAAATGAA
GTATCAATAAGCAGTACATGTACAGTATTTGCAGAGTCTGAGGTTATATCACATTTATCTGAAAATGCAAAAATTGAAGA
TATAGTGGCAGGTATTCATACTTCAGTAGCAAAGAGAGTTTCTAGCCTAGTAAAAAGAATAGGAGTACAAAGAAATGTAG
TTATGGTTGGTGGAGTTGCTAGAAATAGTGGTATTGTAAGAGCTATGGCAAGAGAAATCAACACAGAAATTATTGTACCT
GATATACCTCAATTAACTGGTGCTTTAGGAGCAGCGTTATATGCTTTTGATGAAGCAAAAGAATCACAAAAAGAAGTGAA
AAATATATAA

Upstream 100 bases:

>100_bases
TATTAAAATCTTTAGGCTACGATGAAGAGAAAATAAATAACTTTAAAGATAGTAAAGTTGTAAGATATTAATTAGTAATT
CTAGATTTTTTAAAAGGAGA

Downstream 100 bases:

>100_bases
AAAGAGGGATGAAAATGTCTGAAAAAAAAGAAGCTAGAGTAGTAATTAATGATTTATTAGCTGAACAATATGCAAATGCA
TTTAAAGCTAAAGAAGAAGG

Product: activator of 2-hydroxyisocaproyl-CoA dehydratase

Products: NA

Alternate protein names: 2-hydroxyglutaryl-CoA dehydratase component A [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MLKMYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLEKLYGKTGLAREDIKKVVVTGYGRMNYSDADKQI
SELSCHARGVNFIIPETRTIIDIGGQDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNE
VSISSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVARNSGIVRAMAREINTEIIVP
DIPQLTGALGAALYAFDEAKESQKEVKNI

Sequences:

>Translated_269_residues
MLKMYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLEKLYGKTGLAREDIKKVVVTGYGRMNYSDADKQI
SELSCHARGVNFIIPETRTIIDIGGQDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNE
VSISSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVARNSGIVRAMAREINTEIIVP
DIPQLTGALGAALYAFDEAKESQKEVKNI
>Mature_269_residues
MLKMYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLEKLYGKTGLAREDIKKVVVTGYGRMNYSDADKQI
SELSCHARGVNFIIPETRTIIDIGGQDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNE
VSISSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVARNSGIVRAMAREINTEIIVP
DIPQLTGALGAALYAFDEAKESQKEVKNI

Specific function: Required for the activation of (R)-2-hydroxyglutaryl-CoA dehydratase. This protein is extremely sensitive towards oxygen [H]

COG id: COG1924

COG function: function code I; Activator of 2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli yjiL and M.jannaschii MJ0004 and MJ0800 [H]

Homologues:

Organism=Escherichia coli, GI87082426, Length=252, Percent_Identity=39.2857142857143, Blast_Score=183, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002731
- InterPro:   IPR008275 [H]

Pfam domain/function: PF01869 BcrAD_BadFG [H]

EC number: NA

Molecular weight: Translated: 28795; Mature: 28795

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKMYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLEKLYGKTGLAREDI
CCEEEEECCCCCCCCCCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
KKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGGQDAKVLKLDNNGRLL
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCEEEEEECCCCCEE
NFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSISSTCTVFAESEVISHLS
EEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEEHHHHHHHHH
ENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVARNSGIVRAMAREINTEIIVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEECCCHHHHHHHHHCCCEEECC
DIPQLTGALGAALYAFDEAKESQKEVKNI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKMYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLEKLYGKTGLAREDI
CCEEEEECCCCCCCCCCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
KKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGGQDAKVLKLDNNGRLL
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCEEEEEECCCCCEE
NFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSISSTCTVFAESEVISHLS
EEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEEHHHHHHHHH
ENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVARNSGIVRAMAREINTEIIVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEECCCHHHHHHHHHCCCEEECC
DIPQLTGALGAALYAFDEAKESQKEVKNI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8365476; 2659350; 7607244; 11106419; 11243821 [H]