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The map label for this gene is asnB [H]

Identifier: 126697960

GI number: 126697960

Start: 462184

End: 463767

Strand: Direct

Name: asnB [H]

Synonym: CD0391

Alternate gene names: 126697960

Gene position: 462184-463767 (Clockwise)

Preceding gene: 126697959

Following gene: 126697964

Centisome position: 10.77

GC content: 33.33

Gene sequence:

>1584_bases
ATGATAAATTTAGAAGAAAAGTATTTAACACGAAAAGACACATATATGATAAAAATTCTAGAATATAATGGACATATTCG
AAACTGGAAAGTTCTCTGTGAAGAACTTGGAATCGATTCTTCATTATGTAGAGATGAGCGTGAACGAGCAATTTTAGTGG
AAGCATATAAGACATGGGGATATGATATGGCAAATCACATGCATGGAATGTTTGCTTTTGCATTATGGGATACTGTGGAA
AAGAAACTCTTCTGCCTAAGAGACCAGTTTGGAGTAAAACCTTTCTATTATTATGAGACTGAAAATGGGAAGCTTCTCTA
TGGTTCATCTATTCGTAAGATTATGGAGCAACCAGGATTTGTGAAAGAGTTAAATGAAGACATGTTACAGATTTATATGT
CTTTCACATATGTAGCAGGTGAAAATACATTTTTCCGTGGAGTAAAGAAGCTTATGCCAGGACATTACCTTGTCTATCAG
AATAAAACAGTATGTATTGGTCGTTATTGGAAACCTGAATTTCATCCTGACAACAGCAAGTCTATTGAGGAATGGACGGA
TGAAATTCATACAACACTTCAGAAAATTATGCCAGAGGTAAAATCAGAGAATGAAACTGCAGAGTTATTCCTATCAGGAG
GTGTTGATTCATCTTATATATTGGCAATGTCAGATATAGAAAAAACAGGTTCATGTGGTTATGAGGAAGAGCGTTTTGAT
GAATCAAAATTAGCACAACAGACAGCTAATATTCTTGGGTGTAAAAATTCGAGATATATAATTACACCTGAAGAGTATTT
TGCGTCAGTACCTTATGTAATGTACCATATGGAGCAACCAACTGGAGATGCATCTGCTATCGTTTTTGCTATTGGATGCA
AGGCAGCAGCTGAAAACACAAATATCTGTTACTCTGGAGAAGGAGCAGATGAGTTTTTTGGTGGATATAATATATACCAT
AAGGCAGAGTGTTATGGAGAGAATTTGAAGACATTTTATATTGGAAATACAAACATTATGAATGAGGGTGAGAAAAGAAA
AATTCTTAAGAAATACAATCCTGATGTGCTTCCTATTGAAGTGGTAAAAGGAATTTATGAAGAAACAGAAGAACTAGATA
CACTTACTAACATGTCAAATGTAGATATTCAAATATGGTTGGAAGGAGATATTTATTTTAATATAGATAAAATGAGTACT
GCAGTTGGATTGGAAGTCAGAATGCCTCTTACAGATACAAGAATATTTGATATTGCATCCAGAATACCATCAGAATATAA
AGTGAAAGGAAAAGAGAATAAGATTGCCTTTCGTACAGCTGCATCAAAAACATTACCAGAAGAGATTGCTTTTAGAAATA
AATTAGGATTTATTGTGCCAATTCGTATATGGATGGCTGATGACCAATATAATAAGGACATCCGAGATAAATTCAATAGT
GAGATAGCAGATAAATTCTTTAACATAGATGAGATTAATGAGATTTTTAATGACTACATTAGTGGAAACTCTGATAATTG
GAGAAAGATTTGGATGATTTACACATTCCTAGTGTGGTATGAAATCTATTTTGTAAAATGTTAA

Upstream 100 bases:

>100_bases
TTTGAAAAAAGTGGATTTTTCAGTGATTATAAGTGAATAAATATATATAAACATTTAATAAATTTAGAAGTTAAATGTTT
TAATACGAAAGGATACACAT

Downstream 100 bases:

>100_bases
TACAAGAGGAAGAATCACCTGAAAGACCATTCAGGTGATTCTTAATGTTTTATAATTAATGTTTTATAATTTTACTTGCT
AATTCTTATGTCTAACCCTT

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCRDERERAILVEAYKTWGYDMANHMHGMFAFALWDTVE
KKLFCLRDQFGVKPFYYYETENGKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
NKTVCIGRYWKPEFHPDNSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSSYILAMSDIEKTGSCGYEEERFD
ESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYHMEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYH
KAECYGENLKTFYIGNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIYFNIDKMST
AVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIAFRNKLGFIVPIRIWMADDQYNKDIRDKFNS
EIADKFFNIDEINEIFNDYISGNSDNWRKIWMIYTFLVWYEIYFVKC

Sequences:

>Translated_527_residues
MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCRDERERAILVEAYKTWGYDMANHMHGMFAFALWDTVE
KKLFCLRDQFGVKPFYYYETENGKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
NKTVCIGRYWKPEFHPDNSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSSYILAMSDIEKTGSCGYEEERFD
ESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYHMEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYH
KAECYGENLKTFYIGNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIYFNIDKMST
AVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIAFRNKLGFIVPIRIWMADDQYNKDIRDKFNS
EIADKFFNIDEINEIFNDYISGNSDNWRKIWMIYTFLVWYEIYFVKC
>Mature_527_residues
MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCRDERERAILVEAYKTWGYDMANHMHGMFAFALWDTVE
KKLFCLRDQFGVKPFYYYETENGKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
NKTVCIGRYWKPEFHPDNSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSSYILAMSDIEKTGSCGYEEERFD
ESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYHMEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYH
KAECYGENLKTFYIGNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIYFNIDKMST
AVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIAFRNKLGFIVPIRIWMADDQYNKDIRDKFNS
EIADKFFNIDEINEIFNDYISGNSDNWRKIWMIYTFLVWYEIYFVKC

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI71993933, Length=423, Percent_Identity=22.4586288416076, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147557, Length=423, Percent_Identity=22.4586288416076, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 61505; Mature: 61505

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCRDERERAILVEAYKTWG
CCCCCHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHCEEEEEEHHHCC
YDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETENGKLLYGSSIRKIMEQPGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHCCCH
VKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQNKTVCIGRYWKPEFHPDNSK
HHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCC
SIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSSYILAMSDIEKTGSCGYEEERFD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHCC
ESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYHMEQPTGDASAIVFAIGCKAAAENT
HHHHHHHHHHHHCCCCCEEEECCHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
NICYSGEGADEFFGGYNIYHKAECYGENLKTFYIGNTNIMNEGEKRKILKKYNPDVLPIE
CEEECCCCCHHHCCCCCEEEEHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEHHH
VVKGIYEETEELDTLTNMSNVDIQIWLEGDIYFNIDKMSTAVGLEVRMPLTDTRIFDIAS
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEHHCCEECEEEECCCCCHHHHHHHH
RIPSEYKVKGKENKIAFRTAASKTLPEEIAFRNKLGFIVPIRIWMADDQYNKDIRDKFNS
HCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHH
EIADKFFNIDEINEIFNDYISGNSDNWRKIWMIYTFLVWYEIYFVKC
HHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCRDERERAILVEAYKTWG
CCCCCHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHCEEEEEEHHHCC
YDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETENGKLLYGSSIRKIMEQPGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHCCCH
VKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQNKTVCIGRYWKPEFHPDNSK
HHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCC
SIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSSYILAMSDIEKTGSCGYEEERFD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHCC
ESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYHMEQPTGDASAIVFAIGCKAAAENT
HHHHHHHHHHHHCCCCCEEEECCHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
NICYSGEGADEFFGGYNIYHKAECYGENLKTFYIGNTNIMNEGEKRKILKKYNPDVLPIE
CEEECCCCCHHHCCCCCEEEEHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEHHH
VVKGIYEETEELDTLTNMSNVDIQIWLEGDIYFNIDKMSTAVGLEVRMPLTDTRIFDIAS
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEHHCCEECEEEECCCCCHHHHHHHH
RIPSEYKVKGKENKIAFRTAASKTLPEEIAFRNKLGFIVPIRIWMADDQYNKDIRDKFNS
HCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHH
EIADKFFNIDEINEIFNDYISGNSDNWRKIWMIYTFLVWYEIYFVKC
HHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]