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The map label for this gene is pcrA [H]

Identifier: 126697900

GI number: 126697900

Start: 394178

End: 396442

Strand: Direct

Name: pcrA [H]

Synonym: CD0328

Alternate gene names: 126697900

Gene position: 394178-396442 (Clockwise)

Preceding gene: 126697899

Following gene: 126697901

Centisome position: 9.19

GC content: 30.2

Gene sequence:

>2265_bases
ATGAATTTAGATACATTAAATCCTGCTCAAAGAGAAGCAGTAGAAAAGACTGAAGGTCCAGTACTTATACTGGCTGGTGC
TGGTTCAGGTAAAACTAAGGTATTAACAACAAGAATAGCATATCTTATAGAAGATAAAGGTGTACAAGCACCAAATATAT
TGGCTATAACATTTACAAACAAAGCTGCAAATGAAATGAGAGAGAGAGTTGAACAAAATATAGGTCCTGAAACTAAGGAT
ATGTGGATAAGTACATTCCATTCTTGCTGTGTTCGTATACTAAGAAAAGATATAAATAAGATTGGTTACAATAGAAGTTT
TGTAATTTATGATTCAGCAGACCAAGTTACATTGGTTAAAGACTGTCTAAAAGAATTAAATTTAAGTGATAAAGTGTTTG
AACCAAAAGCAGTTATAAGTGCAATTTCAGGAGCAAAAGACAAACTTTATACTCCAAAGCAGTTTAAAGATATCAATATG
GCAGATAATAGAATGGTTAAGATTGCAGATATTTATGCGCTATATCAAGACCGTTTAAAAAGAAATAGTGCACTTGACTT
TGATGATTTAATACTTAAAACTGTAGAACTTTTCAAGGCTAATGATGAAGTGTTAGCATATTACAGAAGCAGATTTAGAT
ACATAATGGTAGATGAGTACCAAGATACAAGTAAAGCTCAATATGAATTGATAAAATTATTAGCAAGAGAACATCAAAAT
ATTTGTGTTGTTGGAGATGATGACCAAAGTATTTATGGATGGAGAGGTGCAGATATAAGAAACATACTTGAATTTGAGAA
AGATTATGACAATGTACATGTTGTAAAGTTAGAACAAAATTATAGGTCTACACAAGTGATATTAGATGCAGCTAATAAAG
TCATATCTAATAATATAGAAAGAAAAAGAAAAAAGCTTTGGAGTGAAAAAAAAGAAGGAGAACTTATAAAGATACAATTA
ACTGGTAGCGAAATAGAAGAGGCTGATTTCATTGCTGATTCAATTGCACAAATAGCTAGAAAAGAAAATAGACCTTATAA
GGATTTTGCAGTACTTTATAGAGCAAATGCACAGGCAAGACCTGTAGAGGACGCTTTAAATAGAAGTCAAATTCCATACA
ATATATATGGTGGTACAAAATTCTATGAGAGAAAAGAAATAAAGGATTTGTTGGCGTATTTAAGAGTTATTCAAAATCCA
CAAGATGATATATCTATAAAAAGGATTATAAATGTTCCTAGAAGAGGTATAGGGCTTAGAACTATAGAAAAAATTGAAGA
TAGAGCTAACTTAAAACAGGAAAGTATATACTCAGTATTAATTGATATAGAAACAAATTCAGATATATCTACTAAGGCAA
GGGCAAGTATAAGTGGATTTGTTGATATTATAGGGACTCTTAGAACTATAAAAGAAGTTTATCCTGTTAGCAAACTTATA
GAAAAAGTCTTGGATACTACAGGATATATGGACGAGCTTGTAGAGATAAGAAATAAAAATGAAAAAGATTTAACAGGCAA
AGGTGAAGAAGCTCAAGACAGAATTGATAATTTGAGAGAATTTATTTCTATAGCTTTGGAGTTTGAAAGTAGTAATGATG
ATACTTATGAAAATAAAGACTTAGAGACATTCCTAACAAGTATAGCACTTACATCTGAATCAAATGATGAAGAAGACAAT
GATAGGGTTTCGCTTATGACAATTCATACATCCAAAGGTTTAGAGTTCCCTGTTGTGTTTTTAACAGGTATGGAAGAAGG
GTTATTTCCTATATCAAGAGCTATAAAATCTATGAGTGATTCACAAATAGAAGAAGAGAGAAGACTTTGTTATGTTGGTA
TAACTAGAGCTAAAGAAGAGCTATATATGTCATTGACAGAGAAGAGAACATTATATGGTAAAACAAATGTTGCAATAGCT
TCAAGATTTATGGAAGAACTGCCAGAAGAATGTATAGAAAGATTGTACAAAGTAAAAAAAGAACTAAGCTATTCTAAAGC
AAGTTATAATATGTTAGATAAATATACTAAAAAGTATATGAGTACTATAAGCAAATCTAAAGTTGCAGATAAAGTTAATG
CTACAATAAAAGATTCTAATAAAGAAACTAATCCAGATGATATAAAACTTGGTTCTAAGGTACATCATCCTAAATTTGGG
GTTGGGACAGTGGTTTCTATAATTGGTACAGATGTTACTATAGCTTTTGACCAACAGGGAATTAAAAAAATAAATAAAGA
ATATACAACACTAAATATTTTATAA

Upstream 100 bases:

>100_bases
TATATTGGAAATAATCTATTAATATAATACATAATTATAATTTGAAGTAATTCATATTTTAGATTAAAATATATTTATGG
CTGTTAACAGGAGGAGAAAT

Downstream 100 bases:

>100_bases
TGGAGGTAAATGAGGAATGGATAGTATAAACTTTGCTAAAAACTTAATAGACTTCATATATGATAGTCCGTCGTCTTTTC
ATGCAGTCGAGAGTACTAAG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 754; Mature: 754

Protein sequence:

>754_residues
MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIEDKGVQAPNILAITFTNKAANEMRERVEQNIGPETKD
MWISTFHSCCVRILRKDINKIGYNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVDEYQDTSKAQYELIKLLAREHQN
ICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVKLEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQL
TGSEIEEADFIADSIAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLAYLRVIQNP
QDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDISTKARASISGFVDIIGTLRTIKEVYPVSKLI
EKVLDTTGYMDELVEIRNKNEKDLTGKGEEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDN
DRVSLMTIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMSLTEKRTLYGKTNVAIA
SRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMSTISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFG
VGTVVSIIGTDVTIAFDQQGIKKINKEYTTLNIL

Sequences:

>Translated_754_residues
MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIEDKGVQAPNILAITFTNKAANEMRERVEQNIGPETKD
MWISTFHSCCVRILRKDINKIGYNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVDEYQDTSKAQYELIKLLAREHQN
ICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVKLEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQL
TGSEIEEADFIADSIAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLAYLRVIQNP
QDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDISTKARASISGFVDIIGTLRTIKEVYPVSKLI
EKVLDTTGYMDELVEIRNKNEKDLTGKGEEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDN
DRVSLMTIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMSLTEKRTLYGKTNVAIA
SRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMSTISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFG
VGTVVSIIGTDVTIAFDQQGIKKINKEYTTLNIL
>Mature_754_residues
MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIEDKGVQAPNILAITFTNKAANEMRERVEQNIGPETKD
MWISTFHSCCVRILRKDINKIGYNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVDEYQDTSKAQYELIKLLAREHQN
ICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVKLEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQL
TGSEIEEADFIADSIAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLAYLRVIQNP
QDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDISTKARASISGFVDIIGTLRTIKEVYPVSKLI
EKVLDTTGYMDELVEIRNKNEKDLTGKGEEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDN
DRVSLMTIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMSLTEKRTLYGKTNVAIA
SRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMSTISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFG
VGTVVSIIGTDVTIAFDQQGIKKINKEYTTLNIL

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=755, Percent_Identity=37.6158940397351, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI48994965, Length=648, Percent_Identity=39.9691358024691, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=25.2941176470588, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=751, Percent_Identity=29.0279627163782, Blast_Score=245, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324477, Length=741, Percent_Identity=23.2118758434548, Blast_Score=124, Evalue=6e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 86241; Mature: 86241

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIEDKGVQAPNILAITFTN
CCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
KAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIGYNRSFVIYDSADQVTLVK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
DCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINMADNRMVKIADIYALYQDRLK
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEEHHHHHHHHHHHHHH
RNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVDEYQDTSKAQYELIKLLAREHQN
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
ICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVKLEQNYRSTQVILDAANKVISNNIE
EEEECCCCCCEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
RKRKKLWSEKKEGELIKIQLTGSEIEEADFIADSIAQIARKENRPYKDFAVLYRANAQAR
HHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHEEECCCCCC
PVEDALNRSQIPYNIYGGTKFYERKEIKDLLAYLRVIQNPQDDISIKRIINVPRRGIGLR
CHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
TIEKIEDRANLKQESIYSVLIDIETNSDISTKARASISGFVDIIGTLRTIKEVYPVSKLI
HHHHHHHHHCCCHHCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKVLDTTGYMDELVEIRNKNEKDLTGKGEEAQDRIDNLREFISIALEFESSNDDTYENKD
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCH
LETFLTSIALTSESNDEEDNDRVSLMTIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSD
HHHHHHHHHEECCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHCCH
SQIEEERRLCYVGITRAKEELYMSLTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKK
HHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELSYSKASYNMLDKYTKKYMSTISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCEECCCCCCCCCCC
VGTVVSIIGTDVTIAFDQQGIKKINKEYTTLNIL
HHHHHHHHCCCEEEEECHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIEDKGVQAPNILAITFTN
CCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
KAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIGYNRSFVIYDSADQVTLVK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
DCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINMADNRMVKIADIYALYQDRLK
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEEHHHHHHHHHHHHHH
RNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVDEYQDTSKAQYELIKLLAREHQN
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
ICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVKLEQNYRSTQVILDAANKVISNNIE
EEEECCCCCCEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
RKRKKLWSEKKEGELIKIQLTGSEIEEADFIADSIAQIARKENRPYKDFAVLYRANAQAR
HHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHEEECCCCCC
PVEDALNRSQIPYNIYGGTKFYERKEIKDLLAYLRVIQNPQDDISIKRIINVPRRGIGLR
CHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
TIEKIEDRANLKQESIYSVLIDIETNSDISTKARASISGFVDIIGTLRTIKEVYPVSKLI
HHHHHHHHHCCCHHCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKVLDTTGYMDELVEIRNKNEKDLTGKGEEAQDRIDNLREFISIALEFESSNDDTYENKD
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCH
LETFLTSIALTSESNDEEDNDRVSLMTIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSD
HHHHHHHHHEECCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHCCH
SQIEEERRLCYVGITRAKEELYMSLTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKK
HHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELSYSKASYNMLDKYTKKYMSTISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCEECCCCCCCCCCC
VGTVVSIIGTDVTIAFDQQGIKKINKEYTTLNIL
HHHHHHHHCCCEEEEECHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]