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The map label for this gene is purD [H]

Identifier: 126697795

GI number: 126697795

Start: 287475

End: 288725

Strand: Direct

Name: purD [H]

Synonym: CD0224

Alternate gene names: 126697795

Gene position: 287475-288725 (Clockwise)

Preceding gene: 126697794

Following gene: 126697796

Centisome position: 6.7

GC content: 31.73

Gene sequence:

>1251_bases
ATGAAGATATTGGTTGTAGGCGGTGGCGGAAGAGAGCATGCTATATGCTGGAAACTAAGCAAAGAAAAAAATGTAGAAAA
AATTTATTGTGCACCAGGTAATGCAGGAATTGCAAACGTAGCAGAATGTGTAAATATAGGTGATACTAATATAGAAGAAC
TTTTAAAATTTGCCAAAGAAAATGAAATAGGTCTGACAATAGTAGGACCAGAAGTACCACTAGTTATGGGAATAGTAGAT
GAGTTTGAAAAAGAAGGATTAAGAGTATTTGGACCTAATAAAAAATGTGCTCAACTTGAAGGAAGTAAAGCTTTTTCAAA
AGAATTTATGATAAAACATAACATACCTACTGCAAAATATAAAGAATATACAAACTTAGAAGAAGCTATATCAGAAATAG
ATAGTTTTGGATATCCAGTAGTAATAAAAGCGGACGGATTAGCTGCTGGAAAAGGTGTTGTAATACCTGAAAATAGAGAA
GATGCTATAGCAACTTTAAAAGAAATGATGTCAGATAAGAAGTTTGGAGCTGCTGGAGATAAAATAGTAATTGAAGAGTT
TTTAAAAGGAATAGAAACTTCAATTCTAGCATTTGTAGACAATGATACAATAGTTCCTATGGCAAGTGCAAAAGACCATA
AAAAAGTAAACAACTATGAGCAAGGTCCAAACACTGGAGGAATGGGAACTTTTTCACCTAGTGAAATATATACAGAAGAA
TTAGCAAATAAAGTAAAAGAGACAGTATTAGAAAAAACTTTAGAAGGTTTCAAAAAAGATGGACTTAATTTTAAAGGTAT
TTTATTTGTTGGTCTTATGATAACTGAAGATGGAGAAAAAGTACTTGAATATAATGTAAGATTTGGAGACCCTGAGACAC
AATCTGTTTTATTTAGATTAGAAACAGATTTACATGAGATAATGGAAGCTATACTAGATAATAAGTTAAAAGATATAGAA
ATAAACTATAGTGATGATGAAGCTGTGTGTGTTATGCTTACATCAGGAGGGTATCCAGACAGTTATGAAAAAGGGAAAAT
TATAACTGGTCTTGAAAACTTGGATGATGATATAGTAGTATTCCATAGTGGCACTAAGATGTTTGATGGTAATTTGGTTA
CCAATGGAGGAAGAGTAATTGGTATAACTGCAAAGTCAACTACAGTTAAGGATGCAGCAGAAAAAGTTTATGAAAACATA
AAGAAGATAAACTTTGAAGGAATGCACTATAGAACAGACATAGGAAGATAA

Upstream 100 bases:

>100_bases
GGCTCTATAAAAGATAAAGATTCAATAGAAGCTTGTGACAAGTATGATATGGTTATGGTATTTACAGGAATAAGACACTT
CAAACACTAAGGAGGATATT

Downstream 100 bases:

>100_bases
TTTTTCTAAAATGGAGGATAAGGTATGTTGAATACTGAAAATAAAGACTCAATGGTTAGAAGAGTATTGGTTGAAAAAAG
AGAAGGTTTTGACCTTGAGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECVNIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVD
EFEKEGLRVFGPNKKCAQLEGSKAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNNYEQGPNTGGMGTFSPSEIYTEE
LANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITEDGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIE
INYSDDEAVCVMLTSGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDAAEKVYENI
KKINFEGMHYRTDIGR

Sequences:

>Translated_416_residues
MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECVNIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVD
EFEKEGLRVFGPNKKCAQLEGSKAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNNYEQGPNTGGMGTFSPSEIYTEE
LANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITEDGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIE
INYSDDEAVCVMLTSGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDAAEKVYENI
KKINFEGMHYRTDIGR
>Mature_416_residues
MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECVNIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVD
EFEKEGLRVFGPNKKCAQLEGSKAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNNYEQGPNTGGMGTFSPSEIYTEE
LANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITEDGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIE
INYSDDEAVCVMLTSGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDAAEKVYENI
KKINFEGMHYRTDIGR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=419, Percent_Identity=44.1527446300716, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI4503915, Length=419, Percent_Identity=44.1527446300716, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI209869995, Length=419, Percent_Identity=44.1527446300716, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI209869993, Length=419, Percent_Identity=44.1527446300716, Blast_Score=371, Evalue=1e-103,
Organism=Escherichia coli, GI1790438, Length=422, Percent_Identity=44.7867298578199, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=38.7173396674584, Blast_Score=298, Evalue=5e-81,
Organism=Saccharomyces cerevisiae, GI6321203, Length=436, Percent_Identity=42.8899082568807, Blast_Score=343, Evalue=3e-95,
Organism=Drosophila melanogaster, GI62471619, Length=426, Percent_Identity=43.8967136150235, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24582400, Length=426, Percent_Identity=43.8967136150235, Blast_Score=348, Evalue=3e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45767; Mature: 45767

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECVNIGDTNIEELLKFAKE
CEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHCC
NEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGSKAFSKEFMIKHNIPTAKY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCHHHHCCCCHHHHHHEEEEECCCCHHH
KEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENREDAIATLKEMMSDKKFGAAGD
HHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCC
KIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNNYEQGPNTGGMGTFSPSEIYTEE
HHHHHHHHHHHHHEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITEDGEKVLEYNVRFGDPETQSVLFRL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEEEECCCCCHHHEEEEH
ETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLTSGGYPDSYEKGKIITGLENLDDDIVV
HHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCEEE
FHSGTKMFDGNLVTNGGRVIGITAKSTTVKDAAEKVYENIKKINFEGMHYRTDIGR
EECCCEEECCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure
MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECVNIGDTNIEELLKFAKE
CEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHCC
NEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGSKAFSKEFMIKHNIPTAKY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCHHHHCCCCHHHHHHEEEEECCCCHHH
KEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENREDAIATLKEMMSDKKFGAAGD
HHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCC
KIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNNYEQGPNTGGMGTFSPSEIYTEE
HHHHHHHHHHHHHEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITEDGEKVLEYNVRFGDPETQSVLFRL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEEEECCCCCHHHEEEEH
ETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLTSGGYPDSYEKGKIITGLENLDDDIVV
HHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCEEE
FHSGTKMFDGNLVTNGGRVIGITAKSTTVKDAAEKVYENIKKINFEGMHYRTDIGR
EECCCEEECCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]