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The map label for this gene is purF [H]

Identifier: 126697791

GI number: 126697791

Start: 282915

End: 284282

Strand: Direct

Name: purF [H]

Synonym: CD0220

Alternate gene names: 126697791

Gene position: 282915-284282 (Clockwise)

Preceding gene: 126697790

Following gene: 126697792

Centisome position: 6.59

GC content: 30.99

Gene sequence:

>1368_bases
ATGTGTGGAGTTTTAGGGATTTACTCTAATAAAGATGTAACTAAAGAACTATATTATTCTTTATATTCTATGCAACACAG
GGGGCAAGAAAGCTGTGGTTTAGCTCTTTTAGATGATGGAGAAATTAAGTACAAAAAAGATATGGGATTAGTTGGAGATG
TTTTTAAGGAAAATGAATTATCTAAGTTAAAAGGAAATATAGGGATTGGACATGTTAGATATTCAACAGCTGGAGGAAGT
CATGTTTCTAATTGTCAACCTTTAGTTGGAAGTTGTAGAAAAAGACAATTAGCCATAGCTCATAATGGAAATCTAGTTAA
TGCAAATTATTTAAAAGATATGCTAGAAGAAGATGGATATATGTTCCAAACTAATTCAGATACAGAAGTGATTTTATATA
TACTTGCTAGATATTACAAAGGGGATATAGTAGAAAGTTTAAAAGTGACAATGGATTATATAAAAGGTGCATATGCTCTT
GTAATAATGAGTCAAGAAGAATTGGTTGCAGTTAGAGACCCACATGGATTCAGACCTCTTGTACTTGGTAAAAAGGGTGA
TGAATATATATTTGCATCAGAAAATTGTGCAATAGATATACTTGGCGGAGAAGTTATAAGAGATGTAGAGCCAGGAGAGA
TAATAGTAGTCAAAGATGGAGAACTAAAATCATATTTTTATTCAGAAAATTATAAACCAGTTAAGAAAAGTTGTATATTT
GAACACATATACTTTGCAAGAAATGATGCAACTATAGACAATGTAAATGCATATGAATTTAGAATTAAATGTGGTGAGAG
ATTAGCTCAGAATGAAACTGTAAAGGCAGATATGGTAGTTCCAGTTCCAGATTCAGGTTGGCCAGGAGCTATTGGTTATG
CAAATGCTAGTGGATTAAAAATAAGTGAAGGCTTAGTTAAAAATAGATATGTAGGAAGAACATTTATAAAACCTACACAA
GAAGAAAGAGAAATAGCAGTAAAAATAAAATTAAATCCTCTTTCAACAATAATAAAAGGAAAATCTATAATTTTAGTAGA
CGACTCAATTGTAAGAGGAACTACATCTAAACAATTAGTAAAATCTTTAAGAGAAGCAGGAGCAAAAGAAATTCATCTTA
GAATAACTTCTCCTCCAGTAGCTTACTCTTGTTACTATGGAATAGATACTCCAAATCGCTCTAAATTAATTGCATCAAGT
AACAACGTAGAAGAAATGAGAGAATATATTGGATGTGATAGTTTAAAATTCCTAGATATAGAAGGAATGTTAGATGCAAC
AGAACATAAGTCTACTTTCTGTAAAGCTTGTTTTGATGGAGAATACCCAGTTAAGAAAATTGATAAGGAGGAACTACTTT
CATGTTAA

Upstream 100 bases:

>100_bases
AAAAGTTAGATAAGGACAGATTTAGAAAAGACCTTGGAGACCTTGTAGAAGGTTATACGGAAGTACTTTCAAGAATGAAT
AATAAATAGGAGGCAAATGT

Downstream 100 bases:

>100_bases
CATATAAAGAATCAGGTGTAGATATAGATGAAGGAAATAGAGCAGTAGACCTTATAAAAGGTAAAATAAAAGGAACTTAT
GATGGAAATGTAGTTGGAGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKYKKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGS
HVSNCQPLVGSCRKRQLAIAHNGNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVKDGELKSYFYSENYKPVKKSCIF
EHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKADMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQ
EEREIAVKIKLNPLSTIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPNRSKLIASS
NNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKEELLSC

Sequences:

>Translated_455_residues
MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKYKKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGS
HVSNCQPLVGSCRKRQLAIAHNGNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVKDGELKSYFYSENYKPVKKSCIF
EHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKADMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQ
EEREIAVKIKLNPLSTIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPNRSKLIASS
NNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKEELLSC
>Mature_455_residues
MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKYKKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGS
HVSNCQPLVGSCRKRQLAIAHNGNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVKDGELKSYFYSENYKPVKKSCIF
EHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKADMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQ
EEREIAVKIKLNPLSTIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPNRSKLIASS
NNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKEELLSC

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=503, Percent_Identity=37.7733598409543, Blast_Score=325, Evalue=4e-89,
Organism=Escherichia coli, GI1788651, Length=476, Percent_Identity=40.546218487395, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1790167, Length=201, Percent_Identity=30.3482587064677, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=39.1666666666667, Blast_Score=318, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI17539970, Length=222, Percent_Identity=26.1261261261261, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17532897, Length=218, Percent_Identity=26.605504587156, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17532899, Length=216, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=475, Percent_Identity=36.4210526315789, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6323730, Length=214, Percent_Identity=28.0373831775701, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322745, Length=213, Percent_Identity=27.6995305164319, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=39.2116182572614, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24659604, Length=457, Percent_Identity=40.0437636761488, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI28573187, Length=487, Percent_Identity=38.1930184804928, Blast_Score=334, Evalue=6e-92,
Organism=Drosophila melanogaster, GI21357745, Length=215, Percent_Identity=28.3720930232558, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50769; Mature: 50769

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKYKKDMGLVGDVFKENEL
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCHHHHHCCCCH
SKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHNGNLVNANYLKDMLEEDGY
HHHCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCEEEEEECCCEEEHHHHHHHHHCCCE
MFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYALVIMSQEELVAVRDPHGFRPL
EEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCEE
VLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVKDGELKSYFYSENYKPVKKSCIF
EECCCCCCEEEECCCCEEEEECCHHCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHH
EHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKADMVVPVPDSGWPGAIGYANASGLK
HHHEEECCCCCCCCCCEEEEEEEHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCE
ISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPLSTIIKGKSIILVDDSIVRGTTSKQLV
ECCCHHHCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHCCCEEEEECCCCCCCCCHHHHH
KSLREAGAKEIHLRITSPPVAYSCYYGIDTPNRSKLIASSNNVEEMREYIGCDSLKFLDI
HHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEE
EGMLDATEHKSTFCKACFDGEYPVKKIDKEELLSC
CCCCCCHHHHHHHHHHHCCCCCCHHHCCHHHHHCC
>Mature Secondary Structure
MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKYKKDMGLVGDVFKENEL
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCHHHHHCCCCH
SKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHNGNLVNANYLKDMLEEDGY
HHHCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCEEEEEECCCEEEHHHHHHHHHCCCE
MFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYALVIMSQEELVAVRDPHGFRPL
EEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCEE
VLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVKDGELKSYFYSENYKPVKKSCIF
EECCCCCCEEEECCCCEEEEECCHHCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHH
EHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKADMVVPVPDSGWPGAIGYANASGLK
HHHEEECCCCCCCCCCEEEEEEEHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCE
ISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPLSTIIKGKSIILVDDSIVRGTTSKQLV
ECCCHHHCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHCCCEEEEECCCCCCCCCHHHHH
KSLREAGAKEIHLRITSPPVAYSCYYGIDTPNRSKLIASSNNVEEMREYIGCDSLKFLDI
HHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEE
EGMLDATEHKSTFCKACFDGEYPVKKIDKEELLSC
CCCCCCHHHHHHHHHHHCCCCCCHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]