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The map label for this gene is cls [H]

Identifier: 126697765

GI number: 126697765

Start: 250260

End: 251750

Strand: Direct

Name: cls [H]

Synonym: CD0192

Alternate gene names: 126697765

Gene position: 250260-251750 (Clockwise)

Preceding gene: 126697764

Following gene: 126697766

Centisome position: 5.83

GC content: 28.17

Gene sequence:

>1491_bases
ATGGGAGTAATAGGAACAATATTTCTGTTCTATTTAATTATATCATATCTAGCTGGAGCCATAATTTCTGTGATTATATT
ACTTGAAAATAGAGACCCTGCAAAAACAATGTCTTGGCTATTGATGTTTATTATATTTCCAGGTGTAGGTTTAATGATAT
ATGCTATTTCAGGTAGAAATATTAGAAAAAGAAAATTATTTAAGACTCAAAAACTAGCTAATAATATAAAAGAAAAGAAA
CTATTTGATACATTAGAAAAGATAACGGAAATAGTTGAATTAGAAAAAGAATCTATAAAACAAAATAAGCTTTTAAGAGA
TGAAGAGGATGGTAGTTATAGAAAAAGGGTAATAAATATGTTGTTAAAGACTGGTATGTTTCCCTTTACTAAAAATAATA
AAGTGGATGTATTTGTAGATGGAAATGAAAAGTTTAAAAGACTTATAGAAGATATAAGAGAAGCTAAAGACCATATTCAT
TTAGAGTACTTTATCATCAAAGATAGTGAAATAGGTAGGGTGTTAAAAGAAGAACTTATAAAAAAGGCTAAAGAAGGCAT
TAAGATAAGAATTTTATATGATGATGTAGGATGCTGGAGATTTTGGTTTAATAGAAAATTTTTCCGAGAAATGAGGGAAG
TAGGTATAGAAATTGCAGCATTTTTACCTACAAAATTTCCTATAATAGGAGGGAAATTAAACTATAGAAATCACAGAAAA
ATTGTTGTTATTGATGGAATAATTGGATATACAGGTGGAATAAATATAGGAGATGAATATTTAGGCAAAAATGATAAGTT
TGGATATTGGAGAGATACTCATATTAGGATTAAGGGTATTTCTGTATACATGCTTCAAATGACATTTCTGATTGATTGGT
ATTATACGACAAAAGAAGTATTAGTGACTAAAAATTATTTCCCAAGTGTAGGGAATGTTGGTGAAAGTATGATACAAGTT
GTTGCGAGTGGTCCAGACAGTGACTGGGAAGATATTCATTATGCTTATTTTTCTGCAATATGTCAGGCTAGAAAAAATGT
TTATATAGAAACTCCATATTTTATTCCAGATGAAAGTCTACTAAAAGCTATAAAGAGTGCTGCTCTTAGTGGAGTTGATG
TGAGAATAATATTTCCTAAAATTGCAGACCACAAGATAGTAAATATAGCATCTTATTCATATTTTGAAGAAATATTAAGA
GCAGGTGGTAAAGTTTATTTATATAATAAAGGATTTATACATTCTAAAGTCGTAATAATAGATGATAAAATTGCTTCTGC
AGGTACAGCTAATATGGATTTAAGAAGTTTTATGCTTAACTTTGAGGTAAATGCATTTATCTATGATGAAGAAGTTATTA
GGGTAATGACAGATGATTTCTTTGAGGATTTAAGTTACTGTGAAGAACTTAATTTGGAGGTATTTAAGAATAGAAATATC
ATACAAAAAATCAAAGAATCTGTAGCAAGATTATTTTCACCAATATTATAA

Upstream 100 bases:

>100_bases
GAATCGATAAATTTAAAGAAATGTCTGGATTTGCTCAACAGTACTTATTTTACTATGCAAGAGAACTCGGTATAGGAAAA
TAAATTAAGGGGGAGAAATT

Downstream 100 bases:

>100_bases
AAAATTATTAAAAAAGGTATTGAAAATTATGTGTGATTGGTATATTATAATAATTGTTAGCACTCTATTAGATAGAGTGA
TAATAAATAAAAAGTATATA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFIIFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKK
LFDTLEKITEIVELEKESIKQNKLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEIAAFLPTKFPIIGGKLNYRNHRK
IVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIRIKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQV
VASGPDSDWEDIHYAYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASYSYFEEILR
AGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDEEVIRVMTDDFFEDLSYCEELNLEVFKNRNI
IQKIKESVARLFSPIL

Sequences:

>Translated_496_residues
MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFIIFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKK
LFDTLEKITEIVELEKESIKQNKLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEIAAFLPTKFPIIGGKLNYRNHRK
IVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIRIKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQV
VASGPDSDWEDIHYAYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASYSYFEEILR
AGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDEEVIRVMTDDFFEDLSYCEELNLEVFKNRNI
IQKIKESVARLFSPIL
>Mature_495_residues
GVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFIIFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKL
FDTLEKITEIVELEKESIKQNKLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIHL
EYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEIAAFLPTKFPIIGGKLNYRNHRKI
VVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIRIKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVV
ASGPDSDWEDIHYAYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASYSYFEEILRA
GGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDEEVIRVMTDDFFEDLSYCEELNLEVFKNRNII
QKIKESVARLFSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=507, Percent_Identity=28.7968441814596, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1787007, Length=369, Percent_Identity=26.0162601626016, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 57593; Mature: 57462

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFIIFPGVGLMIYAISGRN
CCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCC
IRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQNKLLRDEEDGSYRKRVINM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIHLEYFIIKDSEIGRVLKEELI
HHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEEEEEEEEECCHHHHHHHHHHH
KKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEIAAFLPTKFPIIGGKLNYRNHRK
HHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCEECCCCCCE
IVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIRIKGISVYMLQMTFLIDWYYTTKEV
EEEEECEECCCCCCCCCHHHCCCCCCCCCEECCEEEEEEHHHHHHHHHHHHHHHEECEEE
LVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHYAYFSAICQARKNVYIETPYFIPDESL
EEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHH
LKAIKSAALSGVDVRIIFPKIADHKIVNIASYSYFEEILRAGGKVYLYNKGFIHSKVVII
HHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCCEEEEEEEE
DDKIASAGTANMDLRSFMLNFEVNAFIYDEEVIRVMTDDFFEDLSYCEELNLEVFKNRNI
ECCCCCCCCCCCHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCH
IQKIKESVARLFSPIL
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFIIFPGVGLMIYAISGRN
CHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCC
IRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQNKLLRDEEDGSYRKRVINM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIHLEYFIIKDSEIGRVLKEELI
HHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEEEEEEEEECCHHHHHHHHHHH
KKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEIAAFLPTKFPIIGGKLNYRNHRK
HHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCEECCCCCCE
IVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIRIKGISVYMLQMTFLIDWYYTTKEV
EEEEECEECCCCCCCCCHHHCCCCCCCCCEECCEEEEEEHHHHHHHHHHHHHHHEECEEE
LVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHYAYFSAICQARKNVYIETPYFIPDESL
EEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHH
LKAIKSAALSGVDVRIIFPKIADHKIVNIASYSYFEEILRAGGKVYLYNKGFIHSKVVII
HHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCCEEEEEEEE
DDKIASAGTANMDLRSFMLNFEVNAFIYDEEVIRVMTDDFFEDLSYCEELNLEVFKNRNI
ECCCCCCCCCCCHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCH
IQKIKESVARLFSPIL
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]