Click here to switch to the map view.

The map label for this gene is pyrB

Identifier: 126697757

GI number: 126697757

Start: 242556

End: 243476

Strand: Direct

Name: pyrB

Synonym: CD0184

Alternate gene names: 126697757

Gene position: 242556-243476 (Clockwise)

Preceding gene: 126697756

Following gene: 126697758

Centisome position: 5.65

GC content: 30.62

Gene sequence:

>921_bases
ATGTTAAAATCAAGAAATTTAATCCAACCAGAAGACTTTTCAATAGAAGAAATAGATGAAATATTAGAATTAGCTCAAAA
AATAATTGACAATCCGTCAAAATATTCTCGTATATGTGAAGGAAAACTATTAGCTACATTATTTTATGAACCATCAACAA
GAACAAGATTAAGCTTTGAATCTGCTATGAACAGATTAGGAGGACGTGTAGTAGGCTTTTCAGAGCCAAATTCATCCTCT
GCATCAAAAGGTGAAACTCTAGGAGATACAATGAGGATAGTATCTGGTTATGTTGATATAATAGCTATGAGACATCCTCA
ATCAGGTGCAGCTTCAGAAGCAGCTAGATATACGGAAGTGCCATTTATAAATGCAGGAGACGGCAAAAACCAGCATCCAA
CTCAAACACTTACAGACTTACTTACTATAAAATCATTAAAAGGAACTTTAGAAAGCCATACAATAGGATTATGTGGCGAT
TTAAAATATGGAAGAACTGTGCATTCTTTGGTTAAGGCTATGGCAAGATATAAAAATACAAAGTTTGTATTTATAGCGCC
TGAAGAGTTAAAAATGCCAGATTATATAAAAGAAGCTATAAAGGGACATGCATATTATGAAACTAATAATTTGGATGATG
TTATAGGAAGCTTGGATGTTTTATATATGACTAGAGTTCAACAAGAAAGATTTGAAGATAAATCAGAGTATGAAAGACTT
AAAAACTATTATATTTTAAATAAAGCTAAATTAGAAAAGGCATCTAAAGATATGTTAGTAATGCATCCATTACCAAGAGT
AAATGAAATAGACATAGATGTAGATAGTGATGATAGAGCTGTATATTTTAAACAAGCTAAATATGGAATGTATGTTAGAA
TGGCTTTAATAATAAAACTTTTAGGTATAAATGAAGACTAA

Upstream 100 bases:

>100_bases
GCGCAATCCCTTGACAGGATAGGGCAAAGGTGGTAAGCTACTAACAGTACAAAAATACTTTTAATTTTAGTTCAGAGAGG
CTAAAAAAGGAGGACGAAAG

Downstream 100 bases:

>100_bases
TAGTTATCATATAATAGTAAAACAAAGGAGATTTTGGGATGTATAAAATTCTGGAAAATATATATATAGGCGAAGATATG
TATAGAATGAAAGTAAAAGG

Product: aspartate carbamoyltransferase catalytic chain

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLLATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSS
ASKGETLGDTMRIVSGYVDIIAMRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSLDVLYMTRVQQERFEDKSEYERL
KNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDSDDRAVYFKQAKYGMYVRMALIIKLLGINED

Sequences:

>Translated_306_residues
MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLLATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSS
ASKGETLGDTMRIVSGYVDIIAMRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSLDVLYMTRVQQERFEDKSEYERL
KNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDSDDRAVYFKQAKYGMYVRMALIIKLLGINED
>Mature_306_residues
MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLLATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSS
ASKGETLGDTMRIVSGYVDIIAMRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSLDVLYMTRVQQERFEDKSEYERL
KNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDSDDRAVYFKQAKYGMYVRMALIIKLLGINED

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=42.9508196721311, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI38788445, Length=317, Percent_Identity=27.7602523659306, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=47.3684210526316, Blast_Score=287, Evalue=8e-79,
Organism=Escherichia coli, GI1790703, Length=142, Percent_Identity=35.2112676056338, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=322, Percent_Identity=41.304347826087, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=270, Percent_Identity=45.1851851851852, Blast_Score=249, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24642586, Length=306, Percent_Identity=42.483660130719, Blast_Score=260, Evalue=8e-70,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_CLOD6 (Q18CS8)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001086654.1
- ProteinModelPortal:   Q18CS8
- SMR:   Q18CS8
- STRING:   Q18CS8
- GeneID:   4915020
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0184
- NMPDR:   fig|1496.1.peg.1479
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- ProtClustDB:   CLSK2534415
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34700; Mature: 34700

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLLATLFYEPSTRTRLSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCEEEHH
SAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIAMRHPQSGAASEAARYTEV
HHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCC
PFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGDLKYGRTVHSLVKAMARYKNT
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCC
KFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSLDVLYMTRVQQERFEDKSEYERL
CEEEECHHHCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDSDDRAVYFKQAKYGMYVRMALIIKL
HHHEEECHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
LGINED
HCCCCC
>Mature Secondary Structure
MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLLATLFYEPSTRTRLSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCEEEHH
SAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIAMRHPQSGAASEAARYTEV
HHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCC
PFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGDLKYGRTVHSLVKAMARYKNT
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCC
KFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSLDVLYMTRVQQERFEDKSEYERL
CEEEECHHHCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDSDDRAVYFKQAKYGMYVRMALIIKL
HHHEEECHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
LGINED
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA