Click here to switch to the map view.

The map label for this gene is metE

Identifier: 126697702

GI number: 126697702

Start: 174387

End: 175580

Strand: Direct

Name: metE

Synonym: CD0130

Alternate gene names: 126697702

Gene position: 174387-175580 (Clockwise)

Preceding gene: 126697700

Following gene: 126697703

Centisome position: 4.06

GC content: 33.84

Gene sequence:

>1194_bases
ATGGCAAGACATTTATTTACGTCAGAATCAGTTACAGAAGGACATCCTGATAAGATATGTGACCAAATATCAGATTCAAT
ATTAGATGCATTATTAGAAAAAGACCCACAATCTAGAGTAGCTTGTGAAACTACAGTTACTACTGGTTTAGTTTTAGTAG
CAGGAGAAATAAGTACATCTGCTTATGTAGACATTCCTAAATTAGTAAGAGAAACAGTAAGAGAAATTGGATATACAAGA
GCAAAATATGGATTTGATTGTGATACTTGCGCAGTTATAACTTCTATTGATGAACAATCTGGAGATATAGCTATGGGAGT
TGATGAAGGTCTAGAAAGTAAGACTGGAGAAGAGATAGAAGAAGAAATAGAAAAAGTTGGAGCTGGAGACCAAGGTATAA
TGTTTGGATTTGCATGTAATGAGACTCCAGAATTAATGCCACTTCCGATATCTTTGGCACATAAATTATCAAGAAGACTT
ACAGAAGTTAGAAAAACAGGTTTAGTTGATTATTTAAGACCAGATGGAAAAACTCAAGTTACTGTTGAATATGAAGGAAG
TAAAGCTGTAAGAGTACATACAGTTCTTATATCAGCTCAACATTGTGAAACAGTATCAAATGATAAAATAAGAGAAGATT
TAATTAATCATGTTATTAAGGAAGTTATACCAGCAGAATTACTTGATGAAGAGACTAAAATTTATATAAATCCAACAGGA
AGATTCGTTATAGGAGGACCACAAGGAGATACAGGTCTTACAGGAAGAAAAATAATAATAGATACTTATGGTGGATATTC
TAGACATGGTGGAGGAGCTTTCTCAGGAAAAGACCCTACAAAAGTTGATAGATCTGCGGCTTATGCAGCTAGATATGTTG
CAAAAAATATTGTTGCGGCTGGTCTTGCAGACAAATGTGAGATAGAACTTGCATATGCTATAGGTATAGCTAGACCATTA
TCTATATTTATAGATACTTTTGGGACAGGCAAGGTTTCAGAAGAAAAACTTGTTGAATTAGTAAATAAACACTTTGATTT
AAGACCGGGAGCGATAATAAGAGACTTAGATTTAAGAAAGCCTCTTTATAAAAAGGTTGCTGCTTATGGTCACTTTGGTA
GAACTGATATAGATTTACCTTGGGAAAGAACTGACAAGGTTGAGCAGTTAAGAAAAGATGCTTTAGGTGAATAA

Upstream 100 bases:

>100_bases
TCTTATAAAGGAGAGGTTTTTTTATTTTGAGTAAATTTCTAAAATTAAAAATAAGGTAAAACCTATAATAAAAATTAAGA
ATAAATAAGGGGGAATAAAA

Downstream 100 bases:

>100_bases
TTTTTAACTAAAAAGCTGTTAGAATGTAATTTTACGATTACTTTCTAACAGCTTTTTTATTAGGAGTTAAATATTTTTAA
TAAATGTTAATTTATCATCA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACETTVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTR
AKYGFDCDTCAVITSIDEQSGDIAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHVIKEVIPAELLDEETKIYINPTG
RFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPL
SIFIDTFGTGKVSEEKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRKDALGE

Sequences:

>Translated_397_residues
MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACETTVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTR
AKYGFDCDTCAVITSIDEQSGDIAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHVIKEVIPAELLDEETKIYINPTG
RFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPL
SIFIDTFGTGKVSEEKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRKDALGE
>Mature_396_residues
ARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACETTVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRA
KYGFDCDTCAVITSIDEQSGDIAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRLT
EVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHVIKEVIPAELLDEETKIYINPTGR
FVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLS
IFIDTFGTGKVSEEKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRKDALGE

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=388, Percent_Identity=56.701030927835, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=55.7591623036649, Blast_Score=426, Evalue=1e-119,
Organism=Escherichia coli, GI1789311, Length=396, Percent_Identity=60.6060606060606, Blast_Score=496, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=55.012853470437, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=54.7557840616967, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=390, Percent_Identity=53.5897435897436, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=52.8497409326425, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=50.3856041131105, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=53.2544378698225, Blast_Score=357, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=51.6034985422741, Blast_Score=350, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=54.0192926045016, Blast_Score=330, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=54.0229885057471, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=54.8302872062663, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320710, Length=388, Percent_Identity=54.3814432989691, Blast_Score=423, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45552159, Length=391, Percent_Identity=53.1969309462916, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580529, Length=391, Percent_Identity=53.1969309462916, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580537, Length=391, Percent_Identity=53.1969309462916, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580533, Length=391, Percent_Identity=53.1969309462916, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580535, Length=391, Percent_Identity=53.1969309462916, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580531, Length=391, Percent_Identity=53.1969309462916, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580527, Length=391, Percent_Identity=53.4526854219949, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580525, Length=391, Percent_Identity=53.4526854219949, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580541, Length=376, Percent_Identity=52.3936170212766, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580539, Length=130, Percent_Identity=53.8461538461538, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_CLOD6 (Q18CL7)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001086599.1
- ProteinModelPortal:   Q18CL7
- SMR:   Q18CL7
- STRING:   Q18CL7
- GeneID:   4913808
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0130
- NMPDR:   fig|1496.1.peg.1536
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   CLSK2534394
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43531; Mature: 43400

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACETTVTTGLVLVAGEISTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCEEEEEECCCCC
AYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGDIAMGVDEGLESKTGEEIE
CEECHHHHHHHHHHHHCCHHHCCCCCCCHHHHEEECCCCCCCEEECHHHCCCCCCCHHHH
EEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRLTEVRKTGLVDYLRPDGKTQV
HHHHHHCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEE
TVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHVIKEVIPAELLDEETKIYINPTG
EEEECCCCEEEEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
RFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GLADKCEIELAYAIGIARPLSIFIDTFGTGKVSEEKLVELVNKHFDLRPGAIIRDLDLRK
CCCCCCEEEEEEHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHCC
PLYKKVAAYGHFGRTDIDLPWERTDKVEQLRKDALGE
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACETTVTTGLVLVAGEISTS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCEEEEEECCCCC
AYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGDIAMGVDEGLESKTGEEIE
CEECHHHHHHHHHHHHCCHHHCCCCCCCHHHHEEECCCCCCCEEECHHHCCCCCCCHHHH
EEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRLTEVRKTGLVDYLRPDGKTQV
HHHHHHCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEE
TVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHVIKEVIPAELLDEETKIYINPTG
EEEECCCCEEEEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
RFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GLADKCEIELAYAIGIARPLSIFIDTFGTGKVSEEKLVELVNKHFDLRPGAIIRDLDLRK
CCCCCCEEEEEEHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHCC
PLYKKVAAYGHFGRTDIDLPWERTDKVEQLRKDALGE
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA