| Definition | Prochlorococcus marinus str. MIT 9301, complete genome. |
|---|---|
| Accession | NC_009091 |
| Length | 1,641,879 |
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The map label for this gene is sqdB [C]
Identifier: 126697193
GI number: 126697193
Start: 1580533
End: 1581726
Strand: Direct
Name: sqdB [C]
Synonym: P9301_18551
Alternate gene names: 126697193
Gene position: 1580533-1581726 (Clockwise)
Preceding gene: 126697192
Following gene: 126697194
Centisome position: 96.26
GC content: 35.18
Gene sequence:
>1194_bases GTGAAAGTTATTGTTCTAGGTGGAGATGGTTTTTGCGGTTGGCCTTGTGCGGTGAATTTAGCAGAGCAAAATCATGATGT AATTATTGTCGACAATTTAAGTCGTAGAAAAATTGATATTGATCTAGAGGTAGAATCTTTAACTCCAATTTCTTCTATAA CAGAACGACTTTCTGCATGGGAAGAAACTGGAGGTAAGCCTATGAGATTTCTTAACATGGATATCTCTAAGCAATATCAA AAATTACTCAATTTGCTCATTGATGAAAAACCAGATTCCGTGATCCATTTTGCAGAACAAAGAGCAGCACCATACTCGAT GAAATCGAGTTTTACCAAAAGATATACAGTAGATAATAATGTTAATGGCACCCACAACCTACTTGCTGCGATAGTAGAGA GTAATTTAGATATTCATGTTGTTCATTTAGGAACAATGGGAGTCTACGGATATGGATCACATAGAGGTGCAACAATTCCA GAAGGTTATCTAAAAGTTGAAGTTCCACAACCTGATGGAAGCCGCTTTGAAGAAGAAATATTACACCCTGCAAGCCCAGG TAGTGTCTACCATATGACTAAAACTCTAGATCAATTATTATTTCTTTACTACAACAAAAATGATCTTGTAAGGATCACTG ATCTACATCAAGGCATTGTTTGGGGAACAAATACAGAAGCAACTTTAAAAGATCCTAGATTGACAAACCGATTTGACTAT GACGGAGATTATGGAACTGTTTTAAACAGATTTCTAATGCAAGCTGCAATTGGATATCCATTAAGTGTTCATGGGACAGG AGGGCAAACAAGAGCATTTATACATATAAAAGACTCTGTAAAATGCGTACAACTTGCTCTTGAAAATCCTCCAAAATCTG GAGAAAGAGTCAAAATCTTTAATCAAATGACTGAGAGTCATCAAGTTGGAGAACTAGCTAAAAAAGTTGCTTCTCTAACT GGAGCTGATATCAATTATTTACCAAATCCAAGGAATGAAGCAGTAGAAAATGATCTAATTGTTGATAATAAATGCTTTAT AGAATTAGGTTTAAACCCAACTACTCTTGATAATGGCCTATTAGAAGAAGTTGTTGAAGTTGCTAAAAAATACTCCAATA GATGTGATCTTAATCGCATACCTTGTGTTTCATCCTGGACAAAAAAACAAGCTGAGGCTATAAAGACTAATTAA
Upstream 100 bases:
>100_bases AAGAAGATTTGAAACTATTTACAATGTTTTTTCGCAAAGTGGAAGCACGCTGTAGTAATTTAATAAATATATTATGAATC TTTTAATTCTGGAGTTCTGA
Downstream 100 bases:
>100_bases AATTTTTGAAATAGTTACCTTAAGAAAGTGAAAATTGCATTGTTTACTGAAACTTTTTTACCTAAAGTTGACGGCATAGT CACAAGACTGACTAAAACAA
Product: sulfolipid (UDP-sulfoquinovose) biosynthesis protein
Products: UDPglucoseal [C]
Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Epimerase; UDP Sulfoquinovose Synthase; Nucleoside-Diphosphate-Sugar Epimerase; DTDP-Glucose 4-6-Dehydratase; NAD Dependent Epimerase/Dehydratase Family Protein
Number of amino acids: Translated: 397; Mature: 397
Protein sequence:
>397_residues MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAWEETGGKPMRFLNMDISKQYQ KLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNNVNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIP EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIFNQMTESHQVGELAKKVASLT GADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGLLEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN
Sequences:
>Translated_397_residues MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAWEETGGKPMRFLNMDISKQYQ KLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNNVNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIP EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIFNQMTESHQVGELAKKVASLT GADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGLLEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN >Mature_397_residues MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAWEETGGKPMRFLNMDISKQYQ KLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNNVNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIP EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIFNQMTESHQVGELAKKVASLT GADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGLLEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN
Specific function: Galactose metabolism; third step. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: NA
Homologues:
Organism=Escherichia coli, GI1786974, Length=150, Percent_Identity=34.6666666666667, Blast_Score=65, Evalue=7e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
NA
Pfam domain/function: NA
EC number: 5.1.3.2 [C]
Molecular weight: Translated: 44345; Mature: 44345
Theoretical pI: Translated: 6.09; Mature: 6.09
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAW CEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH EETGGKPMRFLNMDISKQYQKLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNN HHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHCCCEEEEECCC VNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI CCCHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH LHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY CCCCCCCCEEHHHHHHHHHEEEEECCCCEEEEEECCCCEEECCCCCCEECCCCCCCCCCC DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIF CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEHH NQMTESHQVGELAKKVASLTGADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGL HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEECCEEEEEECCCCCCCCHHH LEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCC >Mature Secondary Structure MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAW CEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH EETGGKPMRFLNMDISKQYQKLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNN HHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHCCCEEEEECCC VNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI CCCHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH LHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY CCCCCCCCEEHHHHHHHHHEEEEECCCCEEEEEECCCCEEECCCCCCEECCCCCCCCCCC DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIF CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEHH NQMTESHQVGELAKKVASLTGADINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGL HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEECCEEEEEECCCCCCCCHHH LEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NAD+ [C]
Metal ions: NA
Kcat value (1/min): 57600 [C]
Specific activity: 233.3
Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]
Substrates: UDPglucose [C]
Specific reaction: UDPglucose <==> UDPglucoseal [C]
General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA