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The map label for this gene is thiG

Identifier: 126697192

GI number: 126697192

Start: 1579568

End: 1580362

Strand: Direct

Name: thiG

Synonym: P9301_18541

Alternate gene names: 126697192

Gene position: 1579568-1580362 (Clockwise)

Preceding gene: 126697191

Following gene: 126697193

Centisome position: 96.2

GC content: 35.35

Gene sequence:

>795_bases
ATGAAAAATTATTCGTCTTTAATAATTGGAGGAAAAAAATTTTCCAGTAGATTAATGGTAGGTACTGGCAAATATAAATC
TTCTCAAGATATGGTAGAAAGTCTGTCAAATTCTGAAACAGAAATTATAACCGTTGCTGTTAGAAGAATTAAAAATGAGC
AGTCAGGAGAAAATTTACTCGAAAAGATCAACTGGGAAAAATACTGGATGCTTCCTAATACAGCTGGTTGTGTTAATTCC
GATGAGGCAGTCAGAATAGCAATTTTAGGTAGAGAACTTGCAAAATTATCTGGTCAAGAGGAAAATAATTTTGTGAAGTT
AGAAGTTATTCCTGACAAAAAGTATTTGCTACCAGATCCAATAGAAACTCTTAAAGCAGCCGAAATTCTAATAAAAAAGG
GTTTCGCTGTACTACCTTATATCAATGCAGATCCTATTCTTGCAAAAAGACTAGAAGAAATAGGTTGTGCAACTGTAATG
CCATTGGGCTCTCCTATAGGCTCAGGGCAAGGTTTGTTAAATTTATCAAATATAAGGATTATTATTGAGAATGCAAAAGT
GCCAGTCATAATTGACGCAGGAATTGGGGTACCTAGTGAAGCTTCTCAAGCTATGGAAATTGGAGCTGATGGTGTCTTAA
TCAATAGTGCAATAGCACAAGCTGCAAATCCCCCTCTAATGGCTCAAGCAATAAATTATAGTGTGAAAGCTGGTAGGCAA
GCTTTTCTGGCAGGAAGAATTAAAAAACAAGACTTTGCAGTAGCAAGTTCGCCGGAAAAAAATATATCTATCTAA

Upstream 100 bases:

>100_bases
TAGATCCAAAAAACAAAACAGCAATTAAAAAAAGTAAGGAGTTAGAAAAAAGAAATAATTACAAACCTTACAAAGGTATT
AAAGATAAGGGATTTTAAAT

Downstream 100 bases:

>100_bases
TTCTCTAAATTGAGAAAAGTTTAAAAAGAAAGTAAAAATTTAATATTTGCTTTTATTTATCCTATTAAGAAAGAAGATTT
GAAACTATTTACAATGTTTT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKNYSSLIIGGKKFSSRLMVGTGKYKSSQDMVESLSNSETEIITVAVRRIKNEQSGENLLEKINWEKYWMLPNTAGCVNS
DEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDPIETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVM
PLGSPIGSGQGLLNLSNIRIIIENAKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
AFLAGRIKKQDFAVASSPEKNISI

Sequences:

>Translated_264_residues
MKNYSSLIIGGKKFSSRLMVGTGKYKSSQDMVESLSNSETEIITVAVRRIKNEQSGENLLEKINWEKYWMLPNTAGCVNS
DEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDPIETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVM
PLGSPIGSGQGLLNLSNIRIIIENAKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
AFLAGRIKKQDFAVASSPEKNISI
>Mature_264_residues
MKNYSSLIIGGKKFSSRLMVGTGKYKSSQDMVESLSNSETEIITVAVRRIKNEQSGENLLEKINWEKYWMLPNTAGCVNS
DEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDPIETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVM
PLGSPIGSGQGLLNLSNIRIIIENAKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
AFLAGRIKKQDFAVASSPEKNISI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=50.3968253968254, Blast_Score=249, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_PROM0 (A3PFF2)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092078.1
- ProteinModelPortal:   A3PFF2
- SMR:   A3PFF2
- STRING:   A3PFF2
- GeneID:   4910945
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18541
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   PMAR167546:P9301ORF_1895-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28465; Mature: 28465

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: ACT_SITE 106-106 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYSSLIIGGKKFSSRLMVGTGKYKSSQDMVESLSNSETEIITVAVRRIKNEQSGENLL
CCCCCEEEEECCHHCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
EKINWEKYWMLPNTAGCVNSDEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDP
HHCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHHHCCCCCCCEEEEEEECCCCCCCCCH
IETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVMPLGSPIGSGQGLLNLSNIRI
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCEEE
IIENAKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
EEECCCCCEEEECCCCCCCHHHHHHHCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHCCHH
AFLAGRIKKQDFAVASSPEKNISI
HHHHHCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKNYSSLIIGGKKFSSRLMVGTGKYKSSQDMVESLSNSETEIITVAVRRIKNEQSGENLL
CCCCCEEEEECCHHCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
EKINWEKYWMLPNTAGCVNSDEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDP
HHCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHHHCCCCCCCEEEEEEECCCCCCCCCH
IETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVMPLGSPIGSGQGLLNLSNIRI
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCEEE
IIENAKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
EEECCCCCEEEECCCCCCCHHHHHHHCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHCCHH
AFLAGRIKKQDFAVASSPEKNISI
HHHHHCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA