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The map label for this gene is purB [H]

Identifier: 126696993

GI number: 126696993

Start: 1383597

End: 1384892

Strand: Direct

Name: purB [H]

Synonym: P9301_16551

Alternate gene names: 126696993

Gene position: 1383597-1384892 (Clockwise)

Preceding gene: 126696991

Following gene: 126696994

Centisome position: 84.27

GC content: 35.11

Gene sequence:

>1296_bases
GTGATCGAGCGTTACACATTACCCGAGATGGGAAAAATCTGGACTGATAGCGCAAAATTCCAGAGTTGGCTTAAGGTTGA
AATAGCTGCATGTGAAGCAAATTTTTCCCTAGGAAAAATTCCTGAGAATGCCATGAAAGAGATACGTTCAAATGCAAAGT
TTGATGAATCTAGAATTGCAGAAATTGAGAAAGAAGTTAAACATGATGTCATAGCATTTCTTACAAGCGTTAATGAATTT
GTAGGAGATTCTGGAAGATATATCCATGTTGGTATGACCAGTAGTGATGTACTTGATACTGGCTTATCTCTTCAGTTAAA
AGATTCTTGCGAATTGTTATCAGAAGAAATTGAGAACCTAGAAAATGAAGTCAGATTATTAGCAAGGAAGCACAAAAATA
CATTAATGATTGGTAGATCTCATGCAATTCATGGGGAGCCAATCTCCTTCGGTTTTAAACTTGCTGGATGGTTAGCAGAA
ATAATAAGGAACAAAAAAAGATTGTTAACACTGAAAGAATCTGTAGCAATTGGACAAATAAGTGGTGCAATGGGAACTTA
CGCTAATACGAATCCCAAAGTAGAACAAATAACTTGTGATTTACTCGGCTTAAAACCTGATACAGCAAGTACTCAGGTCA
TATCGAGAGACAGGCATGCAGAATATGTTCAAACTATTGCGCTAGTTGGCGCTTCTCTAGATAGATTCGCAACTGAAATA
AGAAATTTACAAAGAACTGATGTTTTAGAAGTTGAGGAGGGCTTTACAAAAGGGCAAAAAGGAAGTTCTGCCATGCCTCA
TAAAAGAAATCCTATTAGAAGTGAAAGAGTAAGCGGTTTAGCAAGAATTTTGAGGAGTTACGTCTTAACAGCACTGGACA
ATGTTCCACTTTGGCACGAAAGAGATATAAGCCATAGTTCAAATGAACGTATCATGTTACCTGACGTATCAATCTGTTTG
CATTTTATGCTCAGGGAAATGAAAGATATAGTAAGCAATTTGGAAGTTTATCCAAAAAATATGCTTAAAAATTTAAATAT
ATATGGTGGTGTAATCTTTAGTCAGAAAGTTTTACTTTTGCTTGTAGAAAAGGGGTTGTCTAGAGAAAAAGCTTATAGCT
TAGTCCAAAAAAATGCGCATCAGGCGTGGAATACTGAAAATGGGAATTTCAAACAAAATATAGAGAGAGATAATGAAATA
ATGGATTTTATTGATCAAAGTGACTTGGAAGAATGTTTTAATCCTTCAATTCATCTCAATAATTTAAGTGTAATATGGGA
GAAGTTAGGTATCTAG

Upstream 100 bases:

>100_bases
AATTGTCAGGCAAATATGTTCAATGGCAAGTACATCTTTAAGGTATAGAGCAATAATTCGATTTTGGTTATAAAGTTTAA
ATTGATAATTAATAAAAATT

Downstream 100 bases:

>100_bases
GATTACTGAAAACCTTATCTAAGTTAAACCGGTGTTTTCAGAGGAATAATGACAAAAAACTTTAGGATTGAAAAAGATAG
TATGGGAACAATAAAGGTTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGKIWTDSAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRIAEIEKEVKHDVIAFLTSVNEF
VGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLSEEIENLENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
IIRNKKRLLTLKESVAIGQISGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALDNVPLWHERDISHSSNERIMLPDVSICL
HFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNTENGNFKQNIERDNEI
MDFIDQSDLEECFNPSIHLNNLSVIWEKLGI

Sequences:

>Translated_431_residues
MIERYTLPEMGKIWTDSAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRIAEIEKEVKHDVIAFLTSVNEF
VGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLSEEIENLENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
IIRNKKRLLTLKESVAIGQISGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALDNVPLWHERDISHSSNERIMLPDVSICL
HFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNTENGNFKQNIERDNEI
MDFIDQSDLEECFNPSIHLNNLSVIWEKLGI
>Mature_431_residues
MIERYTLPEMGKIWTDSAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRIAEIEKEVKHDVIAFLTSVNEF
VGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLSEEIENLENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
IIRNKKRLLTLKESVAIGQISGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALDNVPLWHERDISHSSNERIMLPDVSICL
HFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNTENGNFKQNIERDNEI
MDFIDQSDLEECFNPSIHLNNLSVIWEKLGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=432, Percent_Identity=27.0833333333333, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=28.4595300261097, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1787376, Length=323, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=384, Percent_Identity=27.6041666666667, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI32564234, Length=330, Percent_Identity=27.8787878787879, Blast_Score=118, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6323391, Length=443, Percent_Identity=26.410835214447, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24647570, Length=382, Percent_Identity=27.2251308900524, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48797; Mature: 48797

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGKIWTDSAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRIA
CCCCCCCCCCCHHHCCCHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHH
EIEKEVKHDVIAFLTSVNEFVGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLSEEIENL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHH
ENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAEIIRNKKRLLTLKESVAIGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
CCCCCCCCCCCCCHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALDNVPLWHE
HHCHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RDISHSSNERIMLPDVSICLHFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHH
LVEKGLSREKAYSLVQKNAHQAWNTENGNFKQNIERDNEIMDFIDQSDLEECFNPSIHLN
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEC
NLSVIWEKLGI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGKIWTDSAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRIA
CCCCCCCCCCCHHHCCCHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHH
EIEKEVKHDVIAFLTSVNEFVGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLSEEIENL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHH
ENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAEIIRNKKRLLTLKESVAIGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
CCCCCCCCCCCCCHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALDNVPLWHE
HHCHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RDISHSSNERIMLPDVSICLHFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHH
LVEKGLSREKAYSLVQKNAHQAWNTENGNFKQNIERDNEIMDFIDQSDLEECFNPSIHLN
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEC
NLSVIWEKLGI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]