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The map label for this gene is murD [H]

Identifier: 126696889

GI number: 126696889

Start: 1314655

End: 1316085

Strand: Direct

Name: murD [H]

Synonym: P9301_15511

Alternate gene names: 126696889

Gene position: 1314655-1316085 (Clockwise)

Preceding gene: 126696887

Following gene: 126696890

Centisome position: 80.07

GC content: 28.93

Gene sequence:

>1431_bases
ATGATAGATAATCATTCAAATAAAAGAAATATTAATCTCGTAATTGGATTAGGTAAATCTGGATTTTGGGCTGCCAAGTA
TTTGAGAAGCATCAATAAGAGAGTAATTGTTTGGGAAAGTAAAAATGGGATAGAATTCTTAGAAAGAAAAACAGCATTAG
AAGAGCTTAATATAATAGTTTCTCTAAATAAAGAATTTGTATTTGAAGAGATTCAACCTTTTGTGAAAGAGATTGAATCT
GTTGTTGTAAGTCCATCAATACCTTACGACCATAAAACTATTATTGAATTAAAAAAAAAGGGAATTAAAGTAATTGGAGA
AATTAATGTTGCATGGGAAATTTTAAAAGACACAAATTGGATAGGTATTACTGGCACTAATGGCAAGACTACTGTTACTC
ATCTACTAAGCCATATGCTCTGCGATACTGGATTATATGCTCCTTTTGCTGGAAATATTGGTACACCTTTATGTAAATAT
GCTCACTCCAAAAAACATGAAAAAATTGATTGGGTTGTAGCTGAATTAAGCAGTTATCAAATAGAAATATCTCCAGAAGT
AAAACCTAATATTGGAATATGGACAACCTTCACAGAAGATCATCTTGAAAGACATAAAACACTTGAAAACTATTTCAACA
TAAAAAAAAGCTTGTTAGAAAAATCTGATTTTAGAATTTATAATTATGACGATAAAAACCTAAGAAATCACTACACTTCG
CTATCGAGAGGGGTTTGGATAACAACTAGTTTCGATAAATCAAATTTAATTCATTGCGATTATTGGATAGATGATCAAGC
ATATATCGTTGAGAGAGGGAAGAAATTATTCAAACTTGAACATTTTTCTTTAAAAGGAATGCATAATCTTCAAAATCTTT
TATTGGTAATTGCAGCAGCAAGAAAAGTTGGATTATCTGGCAAGAAGATTAAAGATTCTCTATCTAATTACAAACAATTA
CCCCATAGGATGGAAACGATTTATAAAAACAATGATCTGGAAATCATTAATGATAGTAAAGCTACAAATTTTGACTCATC
TATTGCGGGAATAAGTTCAATTGAAGGTCAAATAATAATCATTGCTGGGGGTAGATTAAAAGGGAATGAATATAGTGAGT
GGATAAAAGTTTTAAAGAAAAAAGTTAAATGTGTTTTCCTTTTTGGAGAAAGCTCAAAAGTCCTAAAAATGGCGCTTATT
AATGAAGGATTTAAAGAAAATATTTTTGAATTTTCTGAACTAAAAGAGCTTTTAAATTTTGTTTTTCATTATTTACAAAA
TAATAGGGTTGGAACATTATTATTCTCACCTTCATGCTCTAGTTTTGATCAATTTAAAAATTATGAAGAGCGTGGAGATT
ATTTCAAGAAACTAATAAGTGAAAAATTAAAGGTTAATAAATCCATTCTTTGTTCAAGTATCATTTCTTAA

Upstream 100 bases:

>100_bases
TAATTAACTTTTCTTAGGCGAATGCTATAAATACTGAATAAAAATCTCATGATTGAATTACCCCTTAAACACAATTAATA
TAAATGCAATTATAAATTTA

Downstream 100 bases:

>100_bases
TTTCAGGTCGGTCATCACAACCCCTTACCCTCTAGCAAATATTCACATGATTAGTTAGATTTTGACTATGAATTAACTAC
TAGCAATGAGTGAATCTAAC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MIDNHSNKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESKNGIEFLERKTALEELNIIVSLNKEFVFEEIQPFVKEIES
VVVSPSIPYDHKTIIELKKKGIKVIGEINVAWEILKDTNWIGITGTNGKTTVTHLLSHMLCDTGLYAPFAGNIGTPLCKY
AHSKKHEKIDWVVAELSSYQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHYTS
LSRGVWITTSFDKSNLIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAARKVGLSGKKIKDSLSNYKQL
PHRMETIYKNNDLEIINDSKATNFDSSIAGISSIEGQIIIIAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALI
NEGFKENIFEFSELKELLNFVFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNKSILCSSIIS

Sequences:

>Translated_476_residues
MIDNHSNKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESKNGIEFLERKTALEELNIIVSLNKEFVFEEIQPFVKEIES
VVVSPSIPYDHKTIIELKKKGIKVIGEINVAWEILKDTNWIGITGTNGKTTVTHLLSHMLCDTGLYAPFAGNIGTPLCKY
AHSKKHEKIDWVVAELSSYQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHYTS
LSRGVWITTSFDKSNLIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAARKVGLSGKKIKDSLSNYKQL
PHRMETIYKNNDLEIINDSKATNFDSSIAGISSIEGQIIIIAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALI
NEGFKENIFEFSELKELLNFVFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNKSILCSSIIS
>Mature_476_residues
MIDNHSNKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESKNGIEFLERKTALEELNIIVSLNKEFVFEEIQPFVKEIES
VVVSPSIPYDHKTIIELKKKGIKVIGEINVAWEILKDTNWIGITGTNGKTTVTHLLSHMLCDTGLYAPFAGNIGTPLCKY
AHSKKHEKIDWVVAELSSYQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHYTS
LSRGVWITTSFDKSNLIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAARKVGLSGKKIKDSLSNYKQL
PHRMETIYKNNDLEIINDSKATNFDSSIAGISSIEGQIIIIAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALI
NEGFKENIFEFSELKELLNFVFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNKSILCSSIIS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=460, Percent_Identity=26.9565217391304, Blast_Score=151, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 54742; Mature: 54742

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNHSNKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESKNGIEFLERKTALEELNIIV
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCEEEE
SLNKEFVFEEIQPFVKEIESVVVSPSIPYDHKTIIELKKKGIKVIGEINVAWEILKDTNW
ECCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHCCCEEEEECHHEEEEECCCCE
IGITGTNGKTTVTHLLSHMLCDTGLYAPFAGNIGTPLCKYAHSKKHEKIDWVVAELSSYQ
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHEECCCEE
IEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHYTS
EEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
LSRGVWITTSFDKSNLIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAA
HHCCEEEEEECCCCCEEEEEEEECCCHHHHHCCHHHEEEHHHCCCCHHHHHHHHHHHHHH
RKVGLSGKKIKDSLSNYKQLPHRMETIYKNNDLEIINDSKATNFDSSIAGISSIEGQIII
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCHHCCCCEEEE
IAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALINEGFKENIFEFSELKELLNF
EECCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNKSILCSSIIS
HHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDNHSNKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESKNGIEFLERKTALEELNIIV
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCEEEE
SLNKEFVFEEIQPFVKEIESVVVSPSIPYDHKTIIELKKKGIKVIGEINVAWEILKDTNW
ECCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHCCCEEEEECHHEEEEECCCCE
IGITGTNGKTTVTHLLSHMLCDTGLYAPFAGNIGTPLCKYAHSKKHEKIDWVVAELSSYQ
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHEECCCEE
IEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNHYTS
EEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
LSRGVWITTSFDKSNLIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAA
HHCCEEEEEECCCCCEEEEEEEECCCHHHHHCCHHHEEEHHHCCCCHHHHHHHHHHHHHH
RKVGLSGKKIKDSLSNYKQLPHRMETIYKNNDLEIINDSKATNFDSSIAGISSIEGQIII
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCHHCCCCEEEE
IAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALINEGFKENIFEFSELKELLNF
EECCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNKSILCSSIIS
HHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA