Click here to switch to the map view.

The map label for this gene is pfs [H]

Identifier: 126696780

GI number: 126696780

Start: 1212076

End: 1212894

Strand: Direct

Name: pfs [H]

Synonym: P9301_14421

Alternate gene names: 126696780

Gene position: 1212076-1212894 (Clockwise)

Preceding gene: 126696776

Following gene: 126696782

Centisome position: 73.82

GC content: 28.69

Gene sequence:

>819_bases
TTGAAAAATCAATATGCTGAAATTTTGCATATAGGCATTCTTGGAGCAATGCAAGAAGAAATAGGAAATACAATTAATAA
TCTTTATAATATAGAAAAAAAAAGATATGGTGATTTAACGATAGTCTCAGGTGAAATAAAAATTGATAAAATAACAAAAA
AAAAGCTATTTTTGTCGATAGCATGGAGTGGATGGGGGAAGGTAAGTTCTGCTAGAGCATCTACAAGATTAATTGGTCAT
AAATATAAAAGAACAAATATTGATTTTCTATTATTTACTGGAGTTGCGGGCAGTGTAGATAGTCATGTAAGACAATGGGA
TATTATTATCGCTGATAGCTTAATTCAACATGACTTAGATGCAAGACCAATATTTGAAAGATTTCATATACCAGTTTTTA
ATAAAGATGAATTATCTCCCAAGAAAGAATGGTTAGAATGGATTTTCAAGACTTTAAATAAAAATTTTGAAAGTAAAAAA
TTAAAATTTTTTAAAAACTTATACAAAGGACAAATCGGCACAGGTGATCAATTTATATCTAATAAAAAAGAAATTTTAGA
ACTAAAAAAGAATTTACCCAATCTAAAAGCAGTGGAAATGGAAGGAGGTTCCGTAGCTCAAGTAGCGATGCAAGAAGGAA
TTCCTTGGGTAGTTATCAGAGTTATTTCTGATTCAGCAGATGAATCAGCATCTACTGATTTCAGCACTTTTTTGAAGATC
TATAACAAAGTATCTTCAAATCTAATAGAAGTAATCGCAGAAAACTATTTGAAATCTCCAAAGTTTTTCAATGAAAATAA
CTCAATTAGAATTAATTAA

Upstream 100 bases:

>100_bases
AATAAAATTCCCTTTTTAGGAAAATCTGGATAACTTTTAATAGTATTTTTAAGATTTTTTTCAAGCATTATGTAAGATTA
GTGAAGAGTTTTTTAAATAA

Downstream 100 bases:

>100_bases
GTGTAATTAATAGCTTCTTGACATAAAAAAGTGAAAGCTAAAAAAAATTAGATTTTTATGGAGCCAAGCGGACTCGAACC
GCTGACCCCCTGCATGCCAT

Product: nucleoside phosphorylase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKNQYAEILHIGILGAMQEEIGNTINNLYNIEKKRYGDLTIVSGEIKIDKITKKKLFLSIAWSGWGKVSSARASTRLIGH
KYKRTNIDFLLFTGVAGSVDSHVRQWDIIIADSLIQHDLDARPIFERFHIPVFNKDELSPKKEWLEWIFKTLNKNFESKK
LKFFKNLYKGQIGTGDQFISNKKEILELKKNLPNLKAVEMEGGSVAQVAMQEGIPWVVIRVISDSADESASTDFSTFLKI
YNKVSSNLIEVIAENYLKSPKFFNENNSIRIN

Sequences:

>Translated_272_residues
MKNQYAEILHIGILGAMQEEIGNTINNLYNIEKKRYGDLTIVSGEIKIDKITKKKLFLSIAWSGWGKVSSARASTRLIGH
KYKRTNIDFLLFTGVAGSVDSHVRQWDIIIADSLIQHDLDARPIFERFHIPVFNKDELSPKKEWLEWIFKTLNKNFESKK
LKFFKNLYKGQIGTGDQFISNKKEILELKKNLPNLKAVEMEGGSVAQVAMQEGIPWVVIRVISDSADESASTDFSTFLKI
YNKVSSNLIEVIAENYLKSPKFFNENNSIRIN
>Mature_272_residues
MKNQYAEILHIGILGAMQEEIGNTINNLYNIEKKRYGDLTIVSGEIKIDKITKKKLFLSIAWSGWGKVSSARASTRLIGH
KYKRTNIDFLLFTGVAGSVDSHVRQWDIIIADSLIQHDLDARPIFERFHIPVFNKDELSPKKEWLEWIFKTLNKNFESKK
LKFFKNLYKGQIGTGDQFISNKKEILELKKNLPNLKAVEMEGGSVAQVAMQEGIPWVVIRVISDSADESASTDFSTFLKI
YNKVSSNLIEVIAENYLKSPKFFNENNSIRIN

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively [H]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786354, Length=247, Percent_Identity=27.9352226720648, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =3.2.2.9 [H]

Molecular weight: Translated: 31119; Mature: 31119

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQYAEILHIGILGAMQEEIGNTINNLYNIEKKRYGDLTIVSGEIKIDKITKKKLFLSI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEHHEEEEEEE
AWSGWGKVSSARASTRLIGHKYKRTNIDFLLFTGVAGSVDSHVRQWDIIIADSLIQHDLD
EECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
ARPIFERFHIPVFNKDELSPKKEWLEWIFKTLNKNFESKKLKFFKNLYKGQIGTGDQFIS
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHH
NKKEILELKKNLPNLKAVEMEGGSVAQVAMQEGIPWVVIRVISDSADESASTDFSTFLKI
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
YNKVSSNLIEVIAENYLKSPKFFNENNSIRIN
HHHHHHHHHHHHHHHHHCCCCEECCCCCEEEC
>Mature Secondary Structure
MKNQYAEILHIGILGAMQEEIGNTINNLYNIEKKRYGDLTIVSGEIKIDKITKKKLFLSI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEHHEEEEEEE
AWSGWGKVSSARASTRLIGHKYKRTNIDFLLFTGVAGSVDSHVRQWDIIIADSLIQHDLD
EECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
ARPIFERFHIPVFNKDELSPKKEWLEWIFKTLNKNFESKKLKFFKNLYKGQIGTGDQFIS
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHH
NKKEILELKKNLPNLKAVEMEGGSVAQVAMQEGIPWVVIRVISDSADESASTDFSTFLKI
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
YNKVSSNLIEVIAENYLKSPKFFNENNSIRIN
HHHHHHHHHHHHHHHHHCCCCEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA