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The map label for this gene is garL [H]

Identifier: 126696740

GI number: 126696740

Start: 1169916

End: 1170707

Strand: Direct

Name: garL [H]

Synonym: P9301_14021

Alternate gene names: 126696740

Gene position: 1169916-1170707 (Clockwise)

Preceding gene: 126696739

Following gene: 126696741

Centisome position: 71.25

GC content: 31.69

Gene sequence:

>792_bases
ATGATCTCTTCCAAAATTATTAATAAAGAGCTTAATCAAATAAGAAAAAAATTAAATATTGGGATACCAAGTATTGGAAG
TTGGTTGCAATTAAATAATACGTCAGTTGGAGCTATCATTGCCAAAAATAATTTTGACTGGCTTACTCTTGATTTAGAAC
ATGGAGATATTGACGAAGCGGATATACCTCTAATGGTAAATTCGATTCAGTATTATTGTCCATTAGTTTTTGCACGAATA
AGATCACCACAAACAACATATTGTTCGAGAGCATTAGATCTAGGAGTTAACGGAATAATAATTCCAAAAATTGAAAGCTT
TGAGCAAATAAACAATTTAAGAGAAAGTATTTATTATCCCCCTTATGGTTGTAGAGGAGTTGGCTATGCAAATACAAACT
TATTTGGAGAAAATCTGCTGGACCACTTAAACGAACTAAATGAGCCATTCATAGTTCCCATGATAGAGACAAAAAAAGGA
GTTGAGGAACTTGAAAGCATATTACGATGTAAAGGCATAGATGCTATTCTAATTGGACCATACGATCTAAGCGCTTCTTA
TGGAGTACCTGGCGAAATTCAATCAGAAATAGTTAAAGAAAAAGTTGATTATATTTTAAAAACTTGCAAAAAATTTGCTG
TTCCTGCTGGCTTACACATAGTTGAACCTGATGAGAAAAGTTTGAACGAAGCAATAAAAAAAGGATTCACATTTTTACCA
TATAGTATCGATACAGTATTCTTAAGGAAAGCTTCTAAAAAGCCTAATAATATCCATTATCAAAAAAAATGA

Upstream 100 bases:

>100_bases
AGCAGCTTATAATTGTAATATAGATTTTATTTTAAAAAGTTCTTCACTGAATAAATCTCTTCAATTAATACCTAGAATTA
AAATTATTTAATTAATAAAT

Downstream 100 bases:

>100_bases
AAACTATAGGATTAATCCCTGCAAGAATGGGAAGTTCGAGATTACAAGGGAAACCTTTAAAAAAGATAAAAGGTAAAGAG
ATGCTCTTAAGAGTATATGA

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MISSKIINKELNQIRKKLNIGIPSIGSWLQLNNTSVGAIIAKNNFDWLTLDLEHGDIDEADIPLMVNSIQYYCPLVFARI
RSPQTTYCSRALDLGVNGIIIPKIESFEQINNLRESIYYPPYGCRGVGYANTNLFGENLLDHLNELNEPFIVPMIETKKG
VEELESILRCKGIDAILIGPYDLSASYGVPGEIQSEIVKEKVDYILKTCKKFAVPAGLHIVEPDEKSLNEAIKKGFTFLP
YSIDTVFLRKASKKPNNIHYQKK

Sequences:

>Translated_263_residues
MISSKIINKELNQIRKKLNIGIPSIGSWLQLNNTSVGAIIAKNNFDWLTLDLEHGDIDEADIPLMVNSIQYYCPLVFARI
RSPQTTYCSRALDLGVNGIIIPKIESFEQINNLRESIYYPPYGCRGVGYANTNLFGENLLDHLNELNEPFIVPMIETKKG
VEELESILRCKGIDAILIGPYDLSASYGVPGEIQSEIVKEKVDYILKTCKKFAVPAGLHIVEPDEKSLNEAIKKGFTFLP
YSIDTVFLRKASKKPNNIHYQKK
>Mature_263_residues
MISSKIINKELNQIRKKLNIGIPSIGSWLQLNNTSVGAIIAKNNFDWLTLDLEHGDIDEADIPLMVNSIQYYCPLVFARI
RSPQTTYCSRALDLGVNGIIIPKIESFEQINNLRESIYYPPYGCRGVGYANTNLFGENLLDHLNELNEPFIVPMIETKKG
VEELESILRCKGIDAILIGPYDLSASYGVPGEIQSEIVKEKVDYILKTCKKFAVPAGLHIVEPDEKSLNEAIKKGFTFLP
YSIDTVFLRKASKKPNNIHYQKK

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=247, Percent_Identity=29.5546558704453, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1788578, Length=243, Percent_Identity=27.1604938271605, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 29622; Mature: 29622

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSKIINKELNQIRKKLNIGIPSIGSWLQLNNTSVGAIIAKNNFDWLTLDLEHGDIDEA
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECCCCEEEEEECCCCCCCC
DIPLMVNSIQYYCPLVFARIRSPQTTYCSRALDLGVNGIIIPKIESFEQINNLRESIYYP
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCC
PYGCRGVGYANTNLFGENLLDHLNELNEPFIVPMIETKKGVEELESILRCKGIDAILIGP
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHCCCCEEEECC
YDLSASYGVPGEIQSEIVKEKVDYILKTCKKFAVPAGLHIVEPDEKSLNEAIKKGFTFLP
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCEECC
YSIDTVFLRKASKKPNNIHYQKK
CHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MISSKIINKELNQIRKKLNIGIPSIGSWLQLNNTSVGAIIAKNNFDWLTLDLEHGDIDEA
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECCCCEEEEEECCCCCCCC
DIPLMVNSIQYYCPLVFARIRSPQTTYCSRALDLGVNGIIIPKIESFEQINNLRESIYYP
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCC
PYGCRGVGYANTNLFGENLLDHLNELNEPFIVPMIETKKGVEELESILRCKGIDAILIGP
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHCCCCEEEECC
YDLSASYGVPGEIQSEIVKEKVDYILKTCKKFAVPAGLHIVEPDEKSLNEAIKKGFTFLP
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCEECC
YSIDTVFLRKASKKPNNIHYQKK
CHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA